Spark YG™ 570 anti-ATG5 Antibody

Pricing & Availability
Clone
177.19 (See other available formats)
Regulatory Status
RUO
Other Names
Autophagy protein 5
Isotype
Mouse IgG1, κ
177.19_SPKYG_570_ATG5_Antibody_030822
HeLa cells were fixed with 1% paraformaldehyde (PFA) and blocked with 5% FBS for 30 minutes. Then the cells were incubated with 10 µg/mL of anti-ATG5 (clone 177.19) Spark YG™ 570 at room temperature 3 hours in dark. Flash Phalloidin™ Green 488 (Cat. No. 424201) was used to stain F-actin. Nuclei were counterstained with DAPI. The image was captured with a 60X objective.
  • 177.19_SPKYG_570_ATG5_Antibody_030822
    HeLa cells were fixed with 1% paraformaldehyde (PFA) and blocked with 5% FBS for 30 minutes. Then the cells were incubated with 10 µg/mL of anti-ATG5 (clone 177.19) Spark YG™ 570 at room temperature 3 hours in dark. Flash Phalloidin™ Green 488 (Cat. No. 424201) was used to stain F-actin. Nuclei were counterstained with DAPI. The image was captured with a 60X objective.
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847411 25 µg $159.00
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847412 100 µg $335.00
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Description

Autophagy protein 5 (ATG5) is involved in autophagic vesicle formation. ATG5 forms a complex with autophagy protein 12 (ATG12), which has E3 ubiquitin ligase activity, and is associated with autophagosome vesicle formation. ATG5 detaches from membranes immediately before or after autophagosome formation. ATG5 is also involved in mitochondrial quality control after oxidative damage, the innate anti-viral immune response, and apoptosis.

Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat, Drosophila
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
ATG5 peptide conjugated to KLH.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark YG™ 570 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 5 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark YG™ 570 has a maximum excitation of 555 nm and a maximum emission of 570 nm.

Excitation Laser
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Reactivity to Drosophila was only verified with the purified format.

RRID
AB_2924624 (BioLegend Cat. No. 847411)
AB_2924624 (BioLegend Cat. No. 847412)

Antigen Details

Structure
Human ATG5 is a 275 amino acid protein with a molecular mass of 32 kD.
Distribution

Tissue distribution: Ubiquitous.
Cellular distribution: Vacuole, lysosome, and to a lesser extent mitochondria.

Function
ATG5 is involved in many cellular processes including autophagic vesicle formation, mitochondrial quality control after oxidative damage, innate anti-viral immune response, and apoptosis.
Interaction
ATG12, LC3B, ATG16, TECPR1, Autophagy-linked FYVE protein.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance
Molecular Family
Autophagosome Markers
Antigen References

1. Uchiyama Y, et al. 2008. Histochem. Cell Biol. 129:407. PubMed
2. Wu H, et al. 2014. Int. J. Biol. Sci. 10:1072. PubMed
3. Subramani S, Malhotra V. 2013. EMBO Rep. 14(2):143. PubMed

Gene ID
9474 View all products for this Gene ID
UniProt
View information about ATG5 on UniProt.org
Go To Top Version: 1    Revision Date: 03/08/2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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