Purified anti-c-Fos Phospho (Ser32) Antibody

Pricing & Availability
Clone
A22009C (See other available formats)
Regulatory Status
RUO
Other Names
G0S7, p55, cFos, c-Fos, Proto-oncogene, Transcription Factor subunit, AP-1
Isotype
Mouse IgG2b, κ
A.A22009C_PURE_C-Fos_WB_021324
Whole cell extracts (15 µg total protein) from HeLa cells untreated or treated with the Cell Activation Cocktail (without Brefeldin A) (Cat No. 423301) were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane and then untreated (left blot) or LPP-treated (right blot). The blots were probed with Purified anti-c-Fos Phospho (Ser32) (clone A22009C) for 2 hours. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
  • A.A22009C_PURE_C-Fos_WB_021324
    Whole cell extracts (15 µg total protein) from HeLa cells untreated or treated with the Cell Activation Cocktail (without Brefeldin A) (Cat No. 423301) were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane and then untreated (left blot) or LPP-treated (right blot). The blots were probed with Purified anti-c-Fos Phospho (Ser32) (clone A22009C) for 2 hours. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
  • B. A22009C_PURE_C-Fos_ICC_012924
    HeLa cells untreated (top panels), or treated with Cell Activation Cocktail (without Brefeldin A) (Cat. No. 423301) for 4 hours (bottom panels) were fixed and permeabilized with 2% PFA and ice-cold methanol, and then intracellularly stained with Purified anti-c-Fos Phospho (Ser32) (clone A22009C) (right panels) or Purified mouse IgG2b, κ Isotype Control (Cat. No. 402201) (left panels) followed by Alexa Fluor® 647 Goat anti-Mouse IgG (Cat. No. 405322). Nuclei were counterstained with DAPI (Cat. No. 422801) (blue) and Alexa Fluor® 488 anti-Cytokeratin (clone C-11) (Cat. No. 628608) (green). The images were captured in a Revvity Operetta CLS™ High Content Analysis System with a 63X objective. Scale bar: 50 µm
  • C. A22009C_PURE_C-Fos_ICFC_012924
    HeLa cells untreated (negative control, open histogram), or treated with Cell Activation Cocktail (without Brefeldin A) (Cat. No. 423301) for 4 hours (positive control, filled histogram) were fixed and permeabilized using the True-Phos™ Perm Buffer Set (Cat. No. 425401) and intracellularly stained with Purified anti-c-Fos Phospho (Ser32) (clone A22009C) or Purified mouse IgG2b, κ Isotype Control (open histogram, dashed line) (representative histogram for either untreated or treated cells) (Cat. No. 402201) followed by Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322).
  • d. A22009C_PURE_C-Fos_Antibody_IHC-P_062724
    IHC staining of Purified anti-c-Fos Phospho (Ser32) on formalin-fixed paraffin-embedded human brain cortex tissue was performed following antigen retrieval with Citrate buffer (Cat. No. 420902). The tissue sections were incubated with Alexa Fluor® 647 Goat anti-mouse IgG only (Cat. No. 405322) (panel A) or with Purified anti-c-Fos Phospho (Ser32) (clone A22009C) (panel B) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG. Nuclei were counterstained with DAPI (Cat. No. 422801) Scale bar: 50 µm
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631251 25 µg $180.00
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631252 100 µg $455.00
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Description

The proto-oncogene c-Fos is the human homolog of the retroviral v-Fos oncogene, and is a member of the Fos family of transcription factors. c-Fos forms heterodimers with proteins of the Jun family of transcription factors to form the Activator Protein-1 complex (AP-1). This complex is involved in critical cellular processes such as inflammation, cell proliferation, differentiation, survival, hypoxia, bone development and angiogenesis which suggests c-Fos is an important therapeutic target for cancer, vascular remodeling, acute inflammation and autoimmune diseases.  In addition, increases in c-Fos expression and activation are associated with neuronal activity induced by psychoactive drugs. Expression of c-Fos is rapid and transitory, and phosphorylation at residues Ser32 and Thr232 by Erk5 increases protein stability and nuclear localization.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide from human c-Fos phosphorylated at Ser32.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, ICFC, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.012 - 0.1 µg/mL. For immunocytochemistry, a concentration range of 2.5 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For ICFC use we recommend cell fixation/permeabilization with True-Phos™ Perm buffer.

For use of this antibody in immunohistochemistry on formalin-fixed paraffinembedded tissues (IHC-P), we recommend antigen retrieval with Citrate buffer (Cat. No. 420902). However, the antibody also performs well with antigen retrieval with Tris-EDTA (Cat. No. 422703). 

RRID
AB_3097545 (BioLegend Cat. No. 631251)
AB_3097545 (BioLegend Cat. No. 631252)

Antigen Details

Structure
c-Fos is a 380 amino acid length protein. It has a leucine zipper domain that allows it to form heterodimers with members of the Jun family proteins.
Distribution

Non-phosphorylated forms can be found in the endoplasmic reticulum. However, after activation/phosphorylation the protein is translocated to the nucleus.

Function
c-Fos is a subunit of the AP-1 transcription factor. The half-life of the protein is short; however, phosphorylation induces protein stability and translocation to the nucleus.
Interaction
c-Fos interacts with members of the Jun family proteins to form the AP-1 transcription factor.
Ligand/Receptor
As a part of the transcription factor AP-1, it binds to specific DNA sequences to regulate gene transcription
Biology Area
Cell Biology, Cell Proliferation and Viability, Immuno-Oncology, Immunology, Innate Immunity, Neuroscience, Transcription Factors
Molecular Family
Innate Immune Signaling, Phospho-Proteins, Tumor Suppressors
Antigen References
  1. Sasaki T, et al. 2006. Mol Cell. 24:63-75.
  2. Huang K, et al. 2014. Cell Signal. 26:629-38.
  3. Qu X, et al. 2019. Cancer Sci. 110:3183-3196.
  4. Guo JC, et al. 2015. PLoS One. 10:e0120332.
  5. Rodriguez-Berdini L, et al. 2020. J Biol Chem. 295:8808-8818.
Gene ID
2353 View all products for this Gene ID
UniProt
View information about c-Fos on UniProt.org
Go To Top Version: 2    Revision Date: 06/27/2024

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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