Purified anti-CD325 (N-Cadherin) Antibody

Pricing & Availability
Clone
13A9 (See other available formats)
Regulatory Status
RUO
Other Names
CDHN, CDw325, NCAD, N-cadherin 1, Cadherin 2, N-cadherin (neuronal), cadherin-2, calcium-dependent adhesion protein, neuronal, neural cadherin, Neural-cadherin
Isotype
Mouse IgG1, κ
13A9_PURE_CD325_Antibody_WB_1_072018
Western blot of purified anti-CD325 (N-Cadherin) antibody (clone 13A9). Lane 1: Molecular weight marker; Lane 2: 10 µg of human brain membrane lysate; Lane 3: 10 µg of mouse brain membrane lysate; Lane 4: 10 µg of rat brain membrane lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 13A9_PURE_CD325_Antibody_WB_1_072018
    Western blot of purified anti-CD325 (N-Cadherin) antibody (clone 13A9). Lane 1: Molecular weight marker; Lane 2: 10 µg of human brain membrane lysate; Lane 3: 10 µg of mouse brain membrane lysate; Lane 4: 10 µg of rat brain membrane lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Check Availability
844701 25 µg $54.00
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844702 100 µg $124.00
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Description

CD325 (N-cadherin) is important for neuronal migration during development, neurite outgrowth and target finding during differentiation, initial formation of synapses, and synaptic plasticity in mature neurons. CD325 (N-cadherin) is a 130 kD single pass transmembrane protein. Its extracellular region consists of five extracellular cadherin domains and has one cytoplasmic domain. N-cadherin is involved in organogenesis and in the maintenance of organ architecture by contributing to the sorting of heterogeneous cell types and in the cell adhesion needed to form tissues. N-cadherin is expressed by stem cells, myeloblasts, endothelial cells, fibroblasts, and also is expressed in neural and muscle tissues along with some types of carcinoma cells. CD325 associates with the cytoskeleton trough catenin proteins.

Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human N-cadherin cytoplasmic domain fused to recombinant MBP.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IHC-P, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunfluorescence microscopy1,2,3,6, immunohistochemical staining4, and immunoprecipitation2,5,7.

For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 844703) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Wahl JK, et al. 2003. J. Biol. Chem. 278:17269. (WB, IF)
  2. Knudsen KA, et al. 1995. J. Cell Biol. 130:67. (WB, IF, IP)
  3. Machell NH, et al. 2000. Biol. Reprod. 63:797. (WB, IF)
  4. Han AC, et al. 1997. Hum. Pathol. 28:641. (IHC)
  5. Van Aken EH, et al. 2002. Invest Ophthalmol. Vis. Sci. 43:595. (WB, IP)
  6. Tian G, et al. 2009. J. Biol. Chem. 284:18980. (IF)
  7. Nieman MT. 1999. J. Cell Biol. 147:631. (IP, WB)
RRID
AB_2565806 (BioLegend Cat. No. 844701)
AB_2565806 (BioLegend Cat. No. 844702)

Antigen Details

Structure
Member of cadherin family, single pass transmembrane protein, extracellular region consists of five extracellular cadherin domains, one cytoplasmic domain, 130 kD.
Distribution

Cell membrane, stem cells, myeloblasts, endothelial cells, fibroblasts, neural and muscle tissues, and some types of carcinoma cells.

Function
Embryonic development, maintain tissue architecture, cell sorting and cell adhesion in tissues, promote motility, cell junction organization, pre and post synaptic adhesion, and ERK signaling.
Interaction
Catenins.
Cell Type
Mesenchymal Stem Cells, Neural Stem Cells
Biology Area
Cell Biology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Hirano S, et al. 2012. Physiol. Rev. 92:597.

Gene ID
1000 View all products for this Gene ID
UniProt
View information about CD325 on UniProt.org
Go To Top Version: 2    Revision Date: 06/15/2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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