Purified anti-GFAP Antibody

Pricing & Availability
Clone
SMI 24 (See other available formats)
Regulatory Status
RUO
Other Names
Glial fibrillary acidic protein
Isotype
Mouse IgG2b, κ
1_SMI-24_PURE_GFAP_Antibody_IHC_1_010418
IHC staining of purified anti-GFAP antibody (clone SMI 24) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 10X objective. Scale bar: 100 µm
  • 1_SMI-24_PURE_GFAP_Antibody_IHC_1_010418
    IHC staining of purified anti-GFAP antibody (clone SMI 24) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 10X objective. Scale bar: 100 µm
  • 2_SMI-24_PURE_GFAP_Antibody_IHC_2_010418
    IHC staining of purified anti-GFAP antibody (clone SMI 24) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 10X objective. Scale bar: 100 µm
  • 3_SMI-24_PURE_GFAP_Antibody_IHC_3_010418
    IHC staining of purified anti-GFAP antibody (clone SMI 24) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 4_SMI-24_PURE_GFAP_Antibody_IHC_4_010418
    IHC staining of purified anti-GFAP antibody (clone SMI 24) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 5_SMI-24_PURE_GFAP_Antibody_WB_010418
    Western blot of purified anti-GFAP antibody (clone SMI 24). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain lysate; Lane 3: 20 µg of rat brain lysate. The blots were incubated with 1 µg/mL of clone SMI 24 or Mouse IgG2b overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 6_SMI_24_PURE_GFAP_Antibody_IHC-P_122916
    IHC staining of purified anti-GFAP antibody (clone SMI 24) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 100X (Cat. No. 927601), the tissue was incubated with 0.5 µg/mL of the primary antibody for one hour at room temperature. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
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Cat # Size Price Quantity Check Availability
837403 25 µg $112.00
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837404 100 µg $276.00
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Description

Glial fibrillary acidic protein (GFAP) is an intermediate filament (IF) protein that is expressed by numerous cell types of the central nervous system (CNS) including astrocytes and ependymal cells. GFAP has also been found to be expressed in glomeruli and peritubular fibroblasts, Leydig cells of the testis, keratinocytes, osteocytes and chondrocytes and stellate cells of the pancreas and liver. GFAP is a type III IF protein that is closely related to its non-epithelial family members, vimentin, desmin, and peripherin, which are all involved in the structure and function of the cell’s cytoskeleton. GFAP is thought to help to maintain astrocyte mechanical strength, as well as the shape of the cells.

Type III intermediate filaments are highly conserved and contain three domains: the head, rod and tail domains. This rod domain coils around that of another filament to form a dimer, with the N-terminal and C-terminal of each filament aligned. Type III filaments such as GFAP are capable of forming both homodimers and heterodimers; GFAP can polymerize with other type III proteins or with neurofilament light chain protein (NF-L). Interestingly, GFAP and other type III IF proteins cannot assemble with keratins, the type I and II intermediate filaments: in cells that express both proteins, two separate intermediate filament networks form.

To form networks, the initial GFAP dimers combine to make staggered tetramers, which are the basic subunits of an intermediate filament. The non-helical head and tail domains are necessary for filament formation. The head and tail regions have greater variability of sequence and structure. In spite of this increased variability, the head of GFAP contains two conserved arginines and an aromatic residue that are required for proper assembly.

Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemisty, a concentration range of 0.5 - 5.0 μg/mL is suggested. For western blotting, the suggested use of this reagent is 1.0 - 5.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Monoclonal antibody against glial fibrillary acidic protein (GFAP) derived from the Bigner-Eng clone MAb2E1.

SMI 24 provides for general visualization of GFAP in astrocytes and Bergman glia, it detects a more selected group of astrocytomas than mixtures of SMI 23, 24 and 25. The significance of this selectivity has not yet been explored. Possibilities are different characteristics of individual tumors or cooperativity of antibodies.

Multiple protein fragments ranging from 38 to 48 kD have been reported in human CNS lysates resulting from caspase- and calpain-mediated cleavage of GFAP.

RRID
AB_2650685 (BioLegend Cat. No. 837403)
AB_2650685 (BioLegend Cat. No. 837404)

Antigen Details

Structure
GFAP is a 432 amino acid protein with a molecular mass of approximately 50 kD.
Distribution

GFAP is expressed by numerous cell types of the central nervous system (CNS) including astrocytes, ependymal cells, and Bergmann glia cells (unipolar protoplasmic astrocyte). GFAP is expressed in cells lacking fibronectin.

Function
GFAP is a class-III intermediate filament and a structural constituent of the cytoskeleton. It is a cell-specific marker that is used to distinguish astrocytes from other glial cells during the development of the CNS.
Cell Sources
Cytoskeleton and cytosol
Cell Type
Astrocytes
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Antigen References

1. Khakh BS, et al. 2015. Nat. Neurosci. 18(7): 942. [PubMed]
2. Brenner M. 2014. Neurosci. Lett. 565, 7-13. [PubMed]
3. Hol EM, et al. 2015. Curr. Opin. Cell. Biol. 32:121. [PubMed]

Gene ID
2670 View all products for this Gene ID
UniProt
View information about GFAP on UniProt.org

Other Formats

View All GFAP Reagents Request Custom Conjugation
Description Clone Applications
Anti-GFAP SMI 24 IHC-P,WB
Purified anti-GFAP SMI 24 IHC-P,WB
Go To Top Version: 3    Revision Date: 01/02/2020

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