Purified anti-human CD177 Antibody

Pricing & Availability
Clone
MEM-166 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
Neutrophil specific antigen 1, NB1, polycythemia rubra vera 1
Isotype
Mouse IgG1, κ
MEM-166_Pure_020708
Human peripheral blood granulocytes stained with purified MEM-166, followed by anti-mouse IgG FITC
  • MEM-166_Pure_020708
    Human peripheral blood granulocytes stained with purified MEM-166, followed by anti-mouse IgG FITC
Compare all formats
Cat # Size Price Quantity Check Availability
315802 100 µg $114.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD177 is also known as neutrophil specific antigen 1, NB1, and polycythemia rubra vera 1. It is a member of the uPAR family and is a GPI-linked cell surface glycoprotein with a molecular weight of 60 kD. CD177 is expressed on granulocytes and bone marrow progenitors (early erythroblasts, megakaryocytes). It is thought to be involved in allogeneic and autoimmune responses to neutrophils.

Technical data sheet

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human granulocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC, IP, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting5, and immunofluorescence4.

Application References

(PubMed link indicates BioLegend citation)
  1. Leucocyte Typing VII. Mason D, et al. Eds, 2002 Oxford University Press.
  2. von Vietinghoff S, et al. 2007. Blood 109:4487. PubMed
  3. Korkmaz B, et al. 2008. J. Biol. Chem. 283:35976. PubMed
  4. von Vietinghoff S, et al. 2007. Blood 109:4487. (IF)
  5. Jankowska AM, et al. 2011. Haematologica. 96:954. (WB)
Product Citations
  1. Korkmaz B, et al. 2008. J Biol Chem. 283:35976. PubMed
  2. Syrimi E, et al. 2021. iScience. 24:103215. PubMed
  3. Vietinghoff S, et al. 2007. Blood. 109:4487. PubMed
RRID
AB_2072605 (BioLegend Cat. No. 315802)

Antigen Details

Structure
uPAR family, GPI-linked cell surface glycoprotein, 60 kD
Distribution

Granulocytes, bone marrow progenitors (early erythroblasts, megakaryocytes)

Function
Antigen involved in neutrophil allo- and autoimmunity, function unknown
Modification
Glycosylated
Cell Type
Granulocytes, Hematopoietic stem and progenitors, Neutrophils
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Leukocyte Typing VII. Mason D, et al. (Eds.) Oxford University Press (2002)
2. Kissel K, et al. 2001. Eur. J. Immunol. 31:1301.
3. Lalezari P, et al. 1971. J. Clin. Invest. 50:1108.
4. Temerinac S, et al. 2000. Blood 95:2569.

Gene ID
57126 View all products for this Gene ID
UniProt
View information about CD177 on UniProt.org
Go To Top Version: 4    Revision Date: 01/19/2021

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login/Register
Forgot your password? Reset Password
Request an Account