TotalSeq™-C0382 anti-human CD177 Antibody

Product Details

Verified Reactivity

Human, Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human granulocytes

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA

Preparation

The antibody was purified by chromatography and conjugated with TotalSeq™-C oligomer under optimal conditions.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

PG - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-C antibodies are compatible with 10x Genomics Chromium Single Cell Immune Profiling Solution.

To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.

Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting⁵, and immunofluorescence⁴.

Additional Product Notes

TotalSeq™ reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.

The barcode flanking sequences are CGGAGATGTGTATAAGAGACAGNNNNNNNNNN (PCR handle), and NNNNNNNNNCCCATATAAGA*A*A (capture sequence). N represents either randomly selected A, C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.

View more applications data for this product in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)

1. Leucocyte Typing VII. Mason D, et al. Eds, 2002 Oxford University Press.
2. von Vietinghoff S, et al. 2007. Blood 109:4487. PubMed
3. Korkmaz B, et al. 2008. J. Biol. Chem. 283:35976. PubMed
4. von Vietinghoff S, et al. 2007. Blood 109:4487. (IF)
5. Jankowska AM, et al. 2011. Haematologica. 96:954. (WB)

RRID

AB_3097600 (BioLegend Cat. No. 315813)

Antigen Details

Structure

uPAR family, GPI-linked cell surface glycoprotein, 60 kD

Distribution

Granulocytes, bone marrow progenitors (early erythroblasts, megakaryocytes)

Function

Antigen involved in neutrophil allo- and autoimmunity, function unknown

Modification

Glycosylated

Cell Type

Granulocytes, Hematopoietic stem and progenitors, Neutrophils

Biology Area

Immunology

Molecular Family

CD Molecules

Antigen References

1. Leukocyte Typing VII. Mason D, et al. (Eds.) Oxford University Press (2002)
2. Kissel K, et al. 2001. Eur. J. Immunol. 31:1301.
3. Lalezari P, et al. 1971. J. Clin. Invest. 50:1108.
4. Temerinac S, et al. 2000. Blood 95:2569.

Gene ID

57126 View all products for this Gene ID

UniProt

View information about CD177 on UniProt.org

Related Protocols

• TotalSeq™-B or -C with 10x Feature Barcoding Technology

Other Formats