Purified anti-human CD235ab Antibody

Pricing & Availability
Clone
HIR2 (See other available formats)
Regulatory Status
RUO
Workshop
VII 70299
Other Names
Glycophorin A/B, GPA/GPB, GYPA, GYPB
Isotype
Mouse IgG2b, κ
1_HIR2
Human red blood cells stained with CD235a/b PE
  • 1_HIR2
    Human red blood cells stained with CD235a/b PE
  • 2_HIR2_PURE_CD235ab_Antibody_IHC-P_062221.png
    IHC staining of purified anti-human CD235ab antibody (clone HIR2) on formalin-fixed paraffin-embedded human spleen tissue. Following antigen retrieval using 0.1 M Tris-buffered saline with Tween 20 (Cat. No. 925501), the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 goat anti-mouse IgG (green) (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI (blue), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 10x objective. Scale bar: 100 µm
  • 3_HIR2_PURE_CD235ab_Antibody_SB_111623
    SeqIF™ (sequential immunofluorescence) staining on COMET™ of Purified anti-CD235ab (clone HIR2, yellow) on formalin-fixed paraffin-embedded human pancreatic carcinoma at 0.83 µg/mL. Alexa Fluor™ Plus 647 Goat anti-Mouse IgG antibody (Lunaphore, Cat. No. DR647MS) was used as a secondary antibody. Nuclei were counterstained with DAPI (blue). Tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing.
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306602 100 µg $102.00
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Description

The HIR2 antibody reacts with a common epitope of glycophorin A (CD235a) and glycophorin B (CD235b). Glycophorin A is the major sialoglycoprotein expressed on red blood cell membrane, and erythroid precursors. Glycophorin A shares strong homology with glycophorin B. The HIR2 antibody recognizes human RBCs and erythroid precursors and is useful in erythroid cell development studies. Mature, non-nucleated red blood cells are characteristically glycophorin A positive, but CD45 and CD71 negative.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF®, IHC-P - Verified
SB - Community verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For the use of this antibody in spatial biology applications, we have partnered with Lunaphore Technologies for demonstration of our antibodies on the COMET™. The COMET™ platform is an automated, end-to-end spatial biology solution developed for rapid and flexible multiplex tissue profiling. More information on the COMET™ and a complete list of our antibodies that have been demonstrated on the COMET™ can be found here.

Application References

(PubMed link indicates BioLegend citation)
  1. Mason D, et al. Eds. 2002. Leucocyte Typing VII. Oxford University Press. New York.
Product Citations
  1. Arnold DP, et al. 2023. Nat Commun. 14:2884. PubMed
  2. Valet C, et al. 2022. J Clin Invest. 132: . PubMed
  3. Feyaerts D, et al. 2022. Cell Rep Med. 3:100680. PubMed
  4. Azizi E et al. 2018. Cell. 174(5):1293-1308 e36. PubMed
  5. Vivanco Gonzalez N, et al. 2022. STAR Protoc. 3:101280. PubMed
  6. Wastyk HC, et al. 2021. Cell. 184(16):4137-4153.e14. PubMed
  7. Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
  8. Hartmann FJ, et al. 2019. Cell Rep. 28:819. PubMed
  9. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  10. Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed
RRID
AB_314620 (BioLegend Cat. No. 306602)

Antigen Details

Structure
Sialoglycoprotein, 20 kD
Distribution

Erythrocytes

Cell Type
Erythrocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Mason D, et al. Eds. 2002. Leucocyte Typing VII. Oxford University Press. New York.

Gene ID
2993 View all products for this Gene ID
UniProt
View information about CD235ab on UniProt.org
Go To Top Version: 3    Revision Date: 11/16/2023

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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