- Clone
- HCD54 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- ICAM-1, Ly-47
- Isotype
- Mouse IgG1, κ
-
Human peripheral blood lymphocytes stained with purified HCD54, followed by anti-mouse IgG FITC -
MDA-MB231 breast cancer cell line was stained with anti-human CD54, detected with anti-mouse DyLight™ 649, and nuclear counterstained with DAPI. Images were acquired with a TE300 fluorescence microscope with a 20x objective. Data provided by: Er Liu and John Nolan, La Jolla Bioengineering Institute
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 322702 | 100 µg | $112.00 | |||
CD54 is a 85-110 kD type I transmembrane protein also known as ICAM-1. It is expressed on activated endothelial cells, high endothelial venules, T and B cells, monocytes/macrophages, granulocytes, and dendritic cells. The expression of ICAM-1 on the cell surface is potently upregulated by activation; a soluble form of ICAM-1 can be released from the cell surface. CD54 plays a role in cellular adhesion and is involved in inflammation and leukocyte extravasation. CD54 has also been shown to be the major cellular receptor for rhinovirus. ICAM-1 binds to CD11a/CD18 (LFA-1), CD11b/CD18 (Mac-1), CD11c/CD18 (p150, 95) as well as hyaluronan and fibrinogen.
Product Details
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
ICC - Verified
Block - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: in vitro blocking of lymphoctes interaction1. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 322721 & 322722).
-
Application References
(PubMed link indicates BioLegend citation) -
- Evans HG, et al. 2009. Proc. Natl. Acad. Sci. USA. 106:6232. (Block) PubMed
- Product Citations
-
- RRID
-
AB_535974 (BioLegend Cat. No. 322702)
Antigen Details
- Structure
- Type I membrane protein, Ig superfamily, 85-110 kD
- Distribution
-
Activated endothelial cells, high endothelial venules, T cells and B cells, monocytes/macrophages, granulocytes, dendritic cells. Cell surface expression upregulated upon activation; soluble ICAM-1 released from surface.
- Function
- Cellular adhesion, involved in inflammation and immune reactions. CD54 expressed on endothelial cells in areas of inflammation plays a role in leukocyte extravasation. Major cellular receptor for rhinovirus.
- Ligand/Receptor
- CD11a/CD18 (LFA-1), CD11b/CD18 (Mac-1), CD11c/CD18 (p150, 95) and has also been reported to bind hyaluronan and fibrinogen
- Cell Type
- B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Voraberger G, et al.. 1991 J. Immunol. 147:2777.
2. Staunton DE, et al.. 1988. Cell 52:925.
3. Greve JM, et al.. 1989. Cell 56:839. - Gene ID
- 3383 View all products for this Gene ID
- UniProt
- View information about CD54 on UniProt.org
Other Formats
View All CD54 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD54 | HCD54 | FC,ICC,Block |
| Biotin anti-human CD54 | HCD54 | FC |
| PE anti-human CD54 | HCD54 | FC |
| Alexa Fluor® 488 anti-human CD54 | HCD54 | FC |
| APC anti-human CD54 | HCD54 | FC |
| Pacific Blue™ anti-human CD54 | HCD54 | FC |
| Alexa Fluor® 647 anti-human CD54 | HCD54 | FC |
| FITC anti-human CD54 | HCD54 | FC |
| Ultra-LEAF™ Purified anti-human CD54 | HCD54 | FC,ICC,Block |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-human CD54
Human peripheral blood lymphocytes stained with purified HCD... 
MDA-MB231 breast cancer cell line was stained with anti-huma... -
Biotin anti-human CD54
Human peripheral blood lymphocytes stained with biotinylated... -
PE anti-human CD54
Human peripheral blood lymphocytes stained with HCD54 PE -
Alexa Fluor® 488 anti-human CD54
Human peripheral blood lymphocytes stained with HCD54 Alexa ... -
APC anti-human CD54
Human peripheral blood lymphocytes stained with HCD54 APC -
Pacific Blue™ anti-human CD54
Human peripheral blood lymphocytes were stained with CD54 (c... -
Alexa Fluor® 647 anti-human CD54
Human peripheral blood lymphocytes stained with HCD54 Alexa ... -
FITC anti-human CD54
Human peripheral blood lymphocytes stained with HCD54 FITC. -
Ultra-LEAF™ Purified anti-human CD54
Human peripheral blood lymphocytes stained with Ultra-LEAF™ ...
MDA-MB231 breast cancer cell line was stained with anti-huma...