Purified anti-human CD66b Antibody

Pricing & Availability
Clone
G10F5 (See other available formats)
Regulatory Status
RUO
Workshop
VI MA81
Other Names
CD67, CGM6, NCA-95, CEACAM8
Isotype
Mouse IgM, κ
1_G10F5_Purified_070507
Human peripheral blood granulocytes stained with purified G10F5, followed by anti-mouse Igs FITC
  • 1_G10F5_Purified_070507
    Human peripheral blood granulocytes stained with purified G10F5, followed by anti-mouse Igs FITC
  • 2_G10F5_PURE_CD66b_Antibody_IHC-P_1_051617
    Human paraffin-embedded spleen tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris-Buffered Saline 1X with Tween 20 (50 mM, pH 7.6, Cat. No. 925501) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human CD66b (clone G10F5) (red) at 4°C overnight. On the next day, tissue was incubated with Alexa Fluor® 647 anti-mouse IgM antibody (clone RMM-1) antibody (red) for an hour. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
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305102 100 µg $114.00
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Description

CD66b is a 95-100 kD glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6, and NCA-95. CD66b is a member of the immunoglobulin superfamily, carcinoembryonic antigen (CEA)-like subfamily. CD66b, expressed on granulocytes, has been reported to induce activation in neutrophils and to be involved in heterophilic adhesion with CD66c.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen, formalin-fixed paraffin-embedded tissue sections, and spatial biology (IBEX)5,6.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  3. Norling LV, et al. 2012. Arterioscler Thromb Vasc Biol. 32:1970. PubMed
  4. Meinke P, et al. 2015. Neuroimmunol Discord. 25:127. PubMed
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. van der Poel M, et al. 2020. J Immunol. 205:2511. PubMed
  2. Tran AC, et al. 2020. Front Immunol. 11:582833. PubMed
  3. Leader AM, et al. 2021. Cancer Cell. 39:1594. PubMed
  4. Valet C, et al. 2022. J Clin Invest. 132: . PubMed
  5. Poon MML, et al. 2023. Nat Immunol. 24:309. PubMed
  6. Thiriard A, et al. 2023. Front Immunol. 14:1107156. PubMed
  7. Chen J, et al. 2023. J Immunol Res. 2023:5437526. PubMed
  8. Weinhage T, et al. 2020. J Immunol. 205:56. PubMed
  9. Miyamoto DT, et al. 2018. Cancer Discov. 0.533333333. PubMed
  10. Arunachalam PS, et al. 2021. Nature. 596:410. PubMed
  11. Schimek V, et al. 2022. Cell Death Dis. 13:113. PubMed
  12. Taft J, et al. 2021. Cell. 184(17):4447-4463.e20. PubMed
  13. Stras SF, et al. 2020. Developmental Cell. 51(3):357-373.e5.. PubMed
  14. Arnold IC, et al. 2018. J Exp Med. 215:2055. PubMed
  15. Cao Q, et al. 2018. Am J Physiol Renal Physiol. 314:F561. PubMed
  16. Xu G, et al. 2021. Cell Res. 31:1230. PubMed
  17. Mann ER, et al. 2020. Sci Immunol. :5. PubMed
  18. Xu L, et al. 2022. Nat Commun. 13:4892. PubMed
  19. Meinke P, et al. 2015. Neuromuscul Disord. 25:127. PubMed
  20. Li H, et al. 2020. Front Immunol. 11:1777. PubMed
  21. Martin JC, et al. 2020. Cell. 178(6):1493-1508.e20.. PubMed
  22. Eldredge LC, et al. 2019. Am J Physiol Lung Cell Mol Physiol. 317:L49. PubMed
  23. Aymonnier K, et al. 2022. Blood Adv. . PubMed
  24. Governa V, et al. 2017. Clin Cancer Res. 23:3847. PubMed
  25. Ehinger E, et al. 2021. Cardiovasc Res. 117:1166. PubMed
  26. Bartsch YC, et al. 2021. MBio. 12:. PubMed
  27. Pelka K, et al. 2021. Cell. 184:4734. PubMed
  28. Norling L, et al. 2012. Arterioscler Thromb Vasc Biol. 32:1970. PubMed
  29. Fischinger S, et al. 2020. JCI Insight. 5:00. PubMed
  30. De Maeyer RPH, et al. 2020. Nat Immunol. 21:615. PubMed
  31. Yu KK, et al. 2021. JCI Insight. 6:. PubMed
  32. Martin A, et al. 2018. Oncotarget. 9:28364. PubMed
  33. Tiberti S, et al. 2022. Nat Commun. 13:6752. PubMed
  34. Oh J, et al. 2021. Clin Cancer Res. 27:4781. PubMed
  35. Debien V, et al. 2021. Cancers (Basel). 13:. PubMed
  36. Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
  37. Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
RRID
AB_314494 (BioLegend Cat. No. 305102)

Antigen Details

Structure
Ig superfamily, CEA antigen group, GPI-linked glycoprotein, 95-100 kD
Distribution

Granulocytes

Function
Cell adhesion, neutrophil activation
Ligand/Receptor
CD66c
Cell Type
Granulocytes, Neutrophils
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Kuijpers T, et al. 1993. J. Immunol. 151:4934.
2. Kuroki M, et al. 1992. J. Leuk. Biol. 52:551.

Gene ID
1088 View all products for this Gene ID
UniProt
View information about CD66b on UniProt.org
Go To Top Version: 3    Revision Date: 08/25/2022

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