- Clone
- Tü169 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- MHC class II, Major Histocompatibility complex II, human leukocyte antigen, HLA
- Isotype
- Mouse IgG2a, κ
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Human peripheral blood lymphocytes were stained with purified HLA-DQ (clone Tü169) (filled histogram) or mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 361502 | 100 µg | $194.00 | |||
HLA-DQ is heterodimeric cell surface glycoprotein comprised of a 27 kD α (heavy) chain and a 32 kD β (light) chain. In contrast to other MHC class II molecules, both chains of HLA-DQ are polymorphic and the α chain shows a high degree of polymorphism. It is expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells, and other non-professional APCs. In hematopoietic development, HLA-DR and DP are expressed first, followed by HLA-DQ. Variations in the HLA gene expression are crucial to graft survival.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human PBL
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-F - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Tü169 has been reported to react with HLA-DQ1 and HLA-DQ2. It also shows weak reactivity to HLA-DQ3.
Additional reported applications (for the relevant formats) include: immunofluorescence2 and in vitro blocking1. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (contact our custom solutions team). -
Application References
(PubMed link indicates BioLegend citation) -
- Benagiano M, et al. 2012. Proc. Natl. Acad. Sci. USA 109:1222. (Block)
- Rees LE, et al. 2003. Clin. Exp. Immunol. 134:497. (IF)
- Nasi A, et al. 2013. J. Immunol. 191:3090. (FC)
- Turrini R, et al. 2011. Cancer Immunol. Immunother. 60:1639. (FC)
- Product Citations
-
- RRID
-
AB_2563137 (BioLegend Cat. No. 361502)
Antigen Details
- Structure
- Ig superfamily, MHC class II, heterodimeric transmembrane protein
- Distribution
-
B cells, activated T cells, monocytes/macrophages, dendritic cells, other APCs
- Function
- Antigen presentation
- Ligand/Receptor
- CD3/TCR, CD4
- Cell Type
- Antigen-presenting cells, B cells, Dendritic cells, Macrophages, Monocytes, T cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- MHC Antigens
- Antigen References
-
1. Thorsby E. 1974. Transplant. Rev. 18:51.
2. Qvigstad E, et al. 1984. Hum. Immunol. 11:207.
3. Servenius B, et al. 1984. EMBO J. 3:3209.
4. Ottenhoff TH, et al. 1985. Hum. Immunol. 13:105.
5. Strassmann G, et al. 1985. Hum. Immunol. 13:125.
6. Trowsdale J, et al. 1985. Immunol. Rev. 85:5.
7. Dorman J, et al. 2000. Epidemiologic Reviews 22:218.
8. van Lith M, et al. 2010. J. Biol. Chem. 285:40800. - Gene ID
- 3117 View all products for this Gene ID 3119 View all products for this Gene ID
- UniProt
- View information about HLA-DQ on UniProt.org
Other Formats
View All HLA-DQ Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human HLA-DQ | Tü169 | FC,IHC-F |
| FITC anti-human HLA-DQ | Tü169 | FC |
| TotalSeq™-A1059 anti-human HLA-DQ | Tü169 | PG |
| TotalSeq™-B1059 anti-human HLA-DQ | Tü169 | PG |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human HLA-DQ
Human peripheral blood lymphocytes were stained with purifie... -
FITC anti-human HLA-DQ
Human peripheral blood lymphocytes were stained with HLA-DQ ... -
TotalSeq™-A1059 anti-human HLA-DQ
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TotalSeq™-B1059 anti-human HLA-DQ