- Clone
- BVD8-3G11 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interleukin-3, Burst promoting activity, Eosinophil colony stimulating factor (Eo-CSF), Hematopoietic cell growth factor (HCGF), Mast (MGF/MCGF), Multi-colony stimulating (Multi-CSF), P cell stimulating activity (PCSA), Thy1 inducing factor
- Isotype
- Rat IgG1, κ
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 500502 | 500 µg | $262.00 | |||
IL-3 is a highly species-specific pleiotropic factor produced primarily by activated T cells though also by mast cells, keratinocytes, and astrocytes, which stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. The BVD8-3G11 antibody can neutralize the bioactivity of natural or recombinant IL-3.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Yeast-expressed, recombinant human IL-3
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ELISA Capture - Quality tested
WB, IP - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.5-2 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of human IL-3 protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
ELISA1-4 or ELISPOT Capture: The purified BVD8-3G11 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated BVD3-1F9 antibody (Cat. No. 500604) as the detecting antibody.
Additional reported applications include: Western blotting4 and immunoprecipitation4. -
Application References
(PubMed link indicates BioLegend citation) -
- Abrams JS, et al. 1992. Immunol. Rev. 127:5. (ELISA)
- Abrams JS et al. 1994. Eosnophil-active cytokines in human disease: development and use of monoclonal antibodies to il-3, il-5, and GM-CSF. In Eosinophils in Allergy and Inflammation. Gleich GJ and Kay AB, eds. Marcel Dekker, Inc. New York, NY. p. 133. (ELISA)
- Abrams J. 1995. Curr. Prot. Immunol. 6.20. (ELISA)
- Kaushansky K. 1992. J. Clin. Invest. 90:1879. (ELISA, IP, WB)
- Mahanty S, et al. 1992. J. Immunol. 148:3567.
- RRID
-
AB_315103 (BioLegend Cat. No. 500502)
Antigen Details
- Structure
- Cytokine; 14-30 kD (Mammalian)
- Bioactivity
- Proliferation/differentiation of almost all types of hematopoietic progenitors into granulocytes, macrophages, erythroid cells, megakaryocytes, mast cell colonies; induces expression of 20-α-steroid dehydrogenase, histidine, ornithine decarboxylase.
- Cell Sources
- Activated T cells, mast cells, eosinophils, keratinocytes, NK cells, endothelial cells.
- Cell Targets
- Erythroid cells, megakaryocytes, neutrophils, eosinophils, basophils, mast cells, monocytic lineages.
- Receptors
- IL-3R, α subunit (CD123), common β subunit (CDw131).
- Biology Area
- Cell Biology, Stem Cells
- Molecular Family
- Growth Factors, Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Frendl G. 1992. Int. J. Immunopharmacol. 14:421.
3. Ihle J. 1992. Chem. Immunology 51:65. - Regulation
- Upregulated by T cell stimulation; downregulated by glucocorticoids.
- Gene ID
- 3562 View all products for this Gene ID
- UniProt
- View information about IL-3 on UniProt.org
Other Formats
View All IL-3 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human IL-3 | BVD8-3G11 | ELISA Capture,IP,WB |
| Ultra-LEAF™ Purified anti-human IL-3 | BVD8-3G11 | Block,IP,WB |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-human IL-3
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