Purified anti-mouse CD252 (OX40L) Antibody

Pricing & Availability
Clone
RM134L (See other available formats)
Regulatory Status
RUO
Other Names
OX-40L, CD134L, CD252, TNFSF4
Isotype
Rat IgG2b, κ
RM134L_Pure_011812
C57BL/6 splenocytes were stimulated with anti-mouse IgM and CD40 antibodies for 3 days, then stained with purified OX40L (clone RM134L) (filled histogram) or rat IgG2b isotype control (open histogram), followed by biotinylated anti-rat IgG and Sav-PE.
  • RM134L_Pure_011812
    C57BL/6 splenocytes were stimulated with anti-mouse IgM and CD40 antibodies for 3 days, then stained with purified OX40L (clone RM134L) (filled histogram) or rat IgG2b isotype control (open histogram), followed by biotinylated anti-rat IgG and Sav-PE.
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108802 500 µg $136.00
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Description

CD252 is a 35 kD member of the TNF superfamily and is also known as OX40 ligand, OX40L, and CD134L. The CD252 is expressed on activated B cells and antigen presenting cells. CD252 interacts with OX40 antigen (CD134), expressed predominantly on activated T cells to increase proliferation and IL-2 production and to enhance proliferation and immunoglobulin secretion in activated B cells.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Rat NRK-52E cell line transfected with mouse OX-40L
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, Block - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The RM134L antibody can block the costimulatory activity of OX40L. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections3, and in vivo and in vitro blocking of OX-40L-OX-40 functional interaction1,2,4. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 108808).

Application References

(PubMed link indicates BioLegend citation)
  1. Akiba H, et al. 1999. J. Immunol. 162:7058. (Block)
  2. Akiba H, et al. 2000. J. Exp. Med. 191:375. (Block)
  3. Hoshino A, et al. 2003. Eur. J. Immunol. 33:861. (IHC)
  4. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Block)
  5. Zheng X, et al. 2010. Invest Ophthalmol Vis Sci. 51:3076. PubMed
Product Citations
  1. Malinczak CA, et al. 2021. J Immunol. 206:1315. PubMed
  2. Li H, et al. 2022. Cancer Cell. 40:36. PubMed
  3. Zheng X, et al. 2010. Invest Ophthalmol Vis Sci . 51:3076. PubMed
  4. Garg G, et al. 2017. Oncotarget. 8:35542. PubMed
RRID
AB_313401 (BioLegend Cat. No. 108802)

Antigen Details

Structure
NGF/TNF superfamily, 35 kD
Distribution

Activated B cells, antigen presenting cells

Function
B-cell/T-cell interaction, costimulation
Ligand/Receptor
OX40 (CD134)
Cell Type
Antigen-presenting cells, B cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Akiba H, et al. 1999. J. Immunol. 162:7058.
2. Stüber E, et al. 1995. Immunity 2:507.
3. Baum PR, et al. 1994. EMBO J. 13:3992.

Gene ID
22164 View all products for this Gene ID
UniProt
View information about CD252 on UniProt.org
Go To Top Version: 2    Revision Date: 02/04/2015

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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