- Clone
- S16009B (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Prf1, Pfp, Pfn, Pfn-1
- Isotype
- Rat IgG2a, κ
-
C57BL/6 splenocytes were fixed, permeabilized, and stained with purified Perforin (Clone S16009B, top) or purified mouse IgG2a, κ isotype control (bottom), followed by anti-rat IgG PE and then anti-mouse NK1.1 APC. -
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 154402 | 500 µg | $382.00 | |||
Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse perforin transfected cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Clone S16009B reacts with perforin from both C57BL/6 and BALB/c strains.
- RRID
-
AB_2721745 (BioLegend Cat. No. 154402)
Antigen Details
- Structure
- 70 kD Protein that oligomerizes for pore formation.
- Distribution
-
CTL, NK (Cytoplasmic Granules)
- Function
- Target cell lysis
- Cell Type
- T cells, NK cells
- Biology Area
- Immunology
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
- Podack ER, et al, 1988, Immunol Rev, 103:203
- Konjar S, et al, 2010. Immunology, 131:257
- Trapani JA, et al, 2002, Nat Rev Immunol, 2:735
- Osinska I, et al. 2014, Cent Eur J Immnol, 39:109
- Gene ID
- 18646 View all products for this Gene ID
- UniProt
- View information about Perforin on UniProt.org
Other Formats
View All Perforin Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse Perforin | S16009B | ICFC |
| APC anti-mouse Perforin | S16009B | ICFC |
| PE anti-mouse Perforin | S16009B | ICFC |
| Pacific Blue™ anti-mouse Perforin | S16009B | ICFC |
| Alexa Fluor® 488 anti-mouse Perforin | S16009B | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-mouse Perforin
C57BL/6 splenocytes were fixed, permeabilized, and stained w... 
-
APC anti-mouse Perforin
C57BL/6 splenocytes were stained with CD49b. Cells were fixe... 
-
PE anti-mouse Perforin
C57BL/6 splenocytes were stained with CD49b. Cells were fix... 
-
Pacific Blue™ anti-mouse Perforin
C57BL/6 splenocytes were stained with anti-mouse CD49b PE, f... -
Alexa Fluor® 488 anti-mouse Perforin
C57BL/6 splenocytes were surface stained with anti-mouse CD4...