Purified anti-XBP-1s Antibody

Pricing & Availability
Clone
143F (See other available formats)
Regulatory Status
RUO
Other Names
X-box binding protein 1
Isotype
Mouse IgG2a, κ
143F_PURE_Xbp1s_1_100120
Total cell lysates (15 µg protein) from HepG2 cells treated without (-) or with (+) 300 nM thapsigargan for the indicated timepoints were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 µg/mL (1:5000 dilution) of purified anti-Xbp-1s antibodies (clones 9D11A43 and 143F) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Equal protein loading was confirmed using Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) used at a 1:25000 dilution (lower). Lane M: Molecular weight ladder.
  • 143F_PURE_Xbp1s_1_100120
    Total cell lysates (15 µg protein) from HepG2 cells treated without (-) or with (+) 300 nM thapsigargan for the indicated timepoints were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 µg/mL (1:5000 dilution) of purified anti-Xbp-1s antibodies (clones 9D11A43 and 143F) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Equal protein loading was confirmed using Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) used at a 1:25000 dilution (lower). Lane M: Molecular weight ladder.
  • 143F_PURE_Xbp1s_2_111810
    Extracts of untreated HepG2 cells (lane 1) and extracts of HepG2 cells treated with 300 nM thapsigargin (TG) for 16 hours (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-Xbp-1s antibody (clone 143F). Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
  • 143F_PURE_Xbp1s_3_070620
    Chromatin Immunoprecipitations (ChIP) were performed using fixed and sonicated chromatin samples from 293T cells treated with tunicamycin (2.0 µg/mL, 8 hr). ChIP was performed with 10.0 µg of chromatin and 2.0 µg of purified anti- XBP-1s antibody (clone 143F) or equal amount of Go-ChIP-Grade™ purified mouse IgG2a, κ isotype ctrl antibody (Cat. No. 401505). The enriched DNA was purified and quantified by real-time qPCR using SYBR Green and primers for the human DNAJB9 exon 1 region and the human α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as percentage of the total amount of input chromatin, which is equivalent to 100%
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647501 25 µg $124.00
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647502 100 µg $293.00
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Description

XBP-1 is a transcription factor containing a bZIP domain. It was first identified because of its ability to bind X-box, a conserved transcriptional element in the promoter of human HLA DR gene. XBP-1 has multiple functions. It controls MHC class II gene regulation and is also essential for differentiation of plasma cells. XBP-1 upregulates as part of the ER stress response, also known as the unfolded protein response. Unspliced XBP-1 is 261 amino acids and migrates on the SDS-PAGE around 33 kD; spliced XBP-1, recognized by clone 143F, is 371 amino acids and migrates around 55 kD.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
XBP-1s recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ChIP - Verified
ICC, IHC-P - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 to 2.0 µg per mL. For ChIP application, use 2 µg of antibody per 10 µg of chromatin per IP. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence14 and immunohistochemical staining of PFA-fixed paraffin-embedded sections1,14.

Additional Product Notes

Using this antibody at a ratio higher than 1:5 (µg antibody per µg of chromatin) will increase background.

Application References

(PubMed link indicates BioLegend citation)
  1. Tsang KY, et al. 2007. PLOS Biol. 5:568. (IHC) PubMed
  2. Farhan H, et al. 2008. EMBO J. 27:2043 (WB) PubMed
  3. Farhan H, et al. 2010. J. Cell Biol. 189:997. PubMed
  4. Feng-Jin G, et al. 2010. Cell Signal. [Epub ahead of print]
  5. Wang L, et al. 2011. Hum Mol Genet. PubMed
  6. Li J, et al. 2011. J. Biol Chem. 286:4912. PubMed
  7. Wang L, et al. 2011. Hum Mol Genet. 20:1008. PubMed
  8. Liu Y, et al. 2011. J Biol Chem. 286:13161. PubMed
  9. Shirley CM, et al. 2011 Blood. 117:6297. PubMed
  10. Vidal RL., et al. 2012. Hum Mol Genet. PubMed
  11. Byrd AE, et al. 2012. J Cell Biol. 196:689. PubMed
  12. Dickie LJ, et al. 2012. Ann rheum Dis. 71:2035. PubMed
  13. Bonetti P, et al. 2013. Blood. 122:2233. PubMed
  14. Maestre L, et al. 2009. Haematologica. 94:419. (IF, IHC)
  15. Rodriguez M, et al. 2014. J Biol Chem. 289:22942. PubMed
Product Citations
  1. Hattori K, et al. 2021. Oncol Lett. 22:680. PubMed
  2. El-Gazzar A, et al. 2023. EMBO Mol Med. 15:e16834. PubMed
  3. Pietrafesa G, et al. 2023. J Transl Med. 21:340. PubMed
  4. Zielke S, et al. 2020. Autophagy. 1:. PubMed
  5. Li J et al. 2018. Cell chemical biology. 25(11):1350-1358 . PubMed
  6. Dickie L, et al. 2012. Ann Rheum Dis. 71:2035. PubMed
  7. Wang L, et al. 2011. Hum Mol Genet. 1.533333333. PubMed
  8. Syx D, et al. 2021. PLoS Genet. 17:e1009339. PubMed
  9. Zang M, et al. 2020. Oncogenesis. 9:31. PubMed
  10. Cai X, et al. 2015. Exp Cell Res. Available online 29 August 2015. PubMed
  11. Byrd A, et al. 2012. J Cell Biol. 196:689. PubMed
  12. Miyoshi A, et al. 2019. FASEB J. 33:3575. PubMed
  13. Li X, et al. 2018. Sci Rep. 7:2013. PubMed
  14. Rinaldi R, et al. 2021. Cells. 10:. PubMed
  15. Lindner P, et al. 2020. Cell Commun Signal. 18:12. PubMed
  16. Shirley C, et al. 2011. Blood. 117:6297. PubMed
  17. Vidal R, et al. 2012. Hum Mol Genet. 2.434027778. PubMed
  18. Luhr M, et al. 2019. J Biol Chem. 294:8197. PubMed
  19. Farhan H, et al. 2010. J Cell Biol. 189:997. PubMed
  20. Li J, et al. 2011. J Biol Chem. 286:4912. PubMed
  21. Liu Y, et al. 2011. J Biol Chem. 286:13161. PubMed
  22. Talty A, et al. 2019. Cell Death Dis. 10:622. PubMed
  23. Walter F, et al. 2022. Front Cell Dev Biol. 10:915065. PubMed
  24. Chen W, et al. 2022. Nat Commun. 13:6796. PubMed
  25. Efimova I, et al. 2021. Front Immunol. 12:643144. PubMed
  26. Devireddy S, et al. 2022. J Cell Biol. 221:. PubMed
RRID
AB_2241743 (BioLegend Cat. No. 647501)
AB_2241743 (BioLegend Cat. No. 647502)

Antigen Details

Distribution

Ubiquitously expressed.

Function
Upregulated by ER stress, IL-6, and IL-4. Downregulation correlates with tumor progression in prostate cancer. Downregulated by PAX5 transcription factor.
Biology Area
Cell Biology
Antigen References

1. Liou HC, et al. 1990. Science 247:1581.
2. Ponath PD, et al. 1993. J. Biol. Chem.268:17074.
3. Takahashi S, et al. 2002. Prostate 50:154.

Gene ID
7494 View all products for this Gene ID
UniProt
View information about XBP-1s on UniProt.org
Go To Top Version: 5    Revision Date: 06/15/2022

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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