- Clone
- 104 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Ly-5.2, LCA
- Isotype
- Mouse (SJL) IgG2a, κ
| Cat # | Size | Price | Quantity | |||
|---|---|---|---|---|---|---|
| 285023 | 50 µg | $288.00 | ||||
CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- B10.S mouse thymocytes and splenocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark Red™ 718 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC
- Recommended Usage
-
Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration. - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3.
- Additional Product Notes
-
For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
-
Application References
(PubMed link indicates BioLegend citation) -
- Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
- Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
- Suzuki K, et al. 2000. Immunity 13:691. (IHC)
- Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
- Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
- Pascal V, et al. 2007. J. Immunol. 179:1751.
- Burman AC, et al. 2007. Blood 110:1064.
- Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
- Phan TG, et al. 2007. Nature Immunol. 8:992.
- Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
- Wen T, et al. 2013. PNAS. 110:6067. PubMed
- Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
- RRID
-
AB_3106289 (BioLegend Cat. No. 285023)
Antigen Details
- Structure
- Protein tyrosine phosphatase (PTP) family, 180-240 kD
- Distribution
-
All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice
- Function
- Phosphatase, T and B cell activation
- Ligand/Receptor
- Galectin-1, CD2, CD3, CD4
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
-
1. Suzuki K, et al. 2000. Immunity 13:691.
- Gene ID
- 19264 View all products for this Gene ID
- UniProt
- View information about CD45.2 on UniProt.org
Other Formats
View All CD45.2 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Biotin anti-mouse CD45.2
C57BL/6 mouse splenocytes stained with biotinylated 104, fol... -
FITC anti-mouse CD45.2
C57BL/6 mouse splenocytes stained with 104 FITC -
PE anti-mouse CD45.2
BALB/c (solid line) and SJL (broken line) splenocytes staine... -
Purified anti-mouse CD45.2
C57BL/6 mouse splenocytes stained with purified 104, followe... -
APC anti-mouse CD45.2
C57BL/6 mouse splenocytes stained with 104 APC -
Alexa Fluor® 488 anti-mouse CD45.2
C57BL/6 mouse splenocytes stained with 104 Alexa Fluor® 488 -
Alexa Fluor® 647 anti-mouse CD45.2
C57BL/6 mouse splenocytes stained with 104 Alexa Fluor® 647 -
Pacific Blue™ anti-mouse CD45.2
C57BL/6 mouse splenocytes stained with Pacific Blue™ 1... -
Alexa Fluor® 700 anti-mouse CD45.2
C57BL/6 mouse splenocytes stained with 104 Alexa Fluor® ... -
APC/Cyanine7 anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with CD45.2 (clone 10... -
PerCP anti-mouse CD45.2
C57BL/6 mouse splenocytes stained with 104 PerCP -
PerCP/Cyanine5.5 anti-mouse CD45.2
C57BL/6 mouse splenocytes stained with 104 PerCP/Cyanine5.5 -
PE/Cyanine7 anti-mouse CD45.2
C57BL/6 mouse splenocytes stained with 104 PE/Cyanine7 -
Brilliant Violet 421™ anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with CD45.2 (clone 10... -
Brilliant Violet 570™ anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with CD45.2 (clone 10... -
Brilliant Violet 650™ anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with CD45.2 (clone 10... -
Brilliant Violet 510™ anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with CD45.2 (clone 10... -
Brilliant Violet 785™ anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with CD45.2 (clone 10... -
Brilliant Violet 605™ anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with CD45.2 (clone 10... -
Purified anti-mouse CD45.2 (Maxpar® Ready)
C57BL/6 mouse bone marrow cells stained with 147Sm-anti-CD45... -
PE/Dazzle™ 594 anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with CD45.2 (clone 10... -
Brilliant Violet 711™ anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with CD45.2 (clone 10... -
Alexa Fluor® 594 anti-mouse CD45.2
BALB/C mouse frozen spleen section was fixed with 4% parafor... -
APC/Fire™ 750 anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with CD45.2 (clone 10... -
TotalSeq™-A0157 anti-mouse CD45.2
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TotalSeq™-B0157 anti-mouse CD45.2
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TotalSeq™-C0157 anti-mouse CD45.2
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Brilliant Violet 750™ anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with CD45.2 (clone 10... -
Spark Blue™ 550 anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with CD45.2 (clone 10... -
Spark NIR™ 685 anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with anti-mouse CD45.... -
Spark UV™ 387 anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with anti-mouse CD45.... -
Spark Blue™ 574 anti-mouse CD45.2 (Flexi-Fluor™)
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Spark Red™ 718 anti-mouse CD45.2 (Flexi-Fluor™)