- Clone
- M3304B06 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-ase, CSPB, CTLA-1, CGL-1, CGL1
- Isotype
- Mouse IgG1, κ
- Barcode Sequence
- ACCAGTAGTGATGAA
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 674605 | 10 µg | $369.00 | |||
Granzyme B is a serine protease that is mainly produced by cytotoxic T cells (CTL) and NK cells. It plays important roles in cytotoxic lymphocyte-mediated apoptosis, chronic inflammation, and impaired wound healing. Granzyme B is packaged in cytoplasmic granules that are exocytosed towards a bound target cell, and subsequently activates multiple protein substrates to induce apoptosis. Perforin facilitates the transfer of Granzyme B into the target cell. Most circulating CD56+ CD8- NK cells and approximately half of circulating CD8+ T cells coexpress both Granzyme A and B. The activation of CD8+ and CD4+ T lymphocytes induces substantial expression of Granzyme B, but not Granzyme A. Recently, Granzyme B has been found to be produced by other cell types such as CD34+ hematopoietic progenitor cells, keratinocytes, basophils, mast cells, plasmacytoid dendritic cells, B cells, and smooth muscle cells. Elevated levels of Granzyme B are also found in some kinds of autoimmune diseases, type 1 diabetes, and cardiovascular diseases.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human Granzyme B produced in the 293E cell line.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA
- Preparation
- The antibody was purified by chromatography and conjugated with TotalSeq™-Bn oligomer under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
SB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining in formalin-fixed, paraffin-embedded (FFPE) lymphoid tissue, and the oligomer sequence is confirmed by sequencing. TotalSeq™-Bn antibodies are compatible with the 10x Visium CytAssist Gene and Protein Expression Assay.
To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution at 14,000xg at 2 − 8°C for 10 minutes before use. Carefully pipette out the liquid avoiding the bottom of the tube when handling. To determine and optimize dilutions for the addition of Totalseq™-Bn antibodies into pre-designed antibody panels, refer to 10x Genomics Custom Add-on Antibody Optimization guide.
Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform. - Additional Product Notes
-
TotalSeq™-Bn reagents are designed to profile protein levels following an optimized protocol in spatial transcriptomics. Compatible spatial biology devices (e.g. Imaging System, 10x Genomics Visium Spatial CytAssist Gene and Protein Expression instruments and reagents) and sequencer (e.g. Illumina analyzers) are required. TotalSeq™-B reagents are not compatible with the 10x Genomics Visium system. The complete barcode sequence may be provided upon request. Please contact technical support for more information, or visit TotalSeq™-Bn Reagents for 10x Genomics Visium CytAssist Gene and Protein Assay.
Antigen Details
- Structure
- 247 amino acids with a molecular weight of approximately 28 kD
- Distribution
-
Cytotoxic T cells, NK cells, and neutrophils.
- Function
- Granzyme B is able to induce target cell apoptosis by activating caspase independent pathways. Granzyme B is induced in CD8+ T lymphocytes with ConA / IL-2 and CD4+ T lymphocytes with anti-CD3/CD28 or anti-CD3/CD46.
- Interaction
- Targets of CTL and NK cells.
- Cell Type
- B cells, Dendritic cells, Neutrophils, NK cells, T cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Neuroscience, Signal Transduction
- Molecular Family
- Enzymes and Regulators, Proteases
- Antigen References
-
1. Edwards KM, et al. 1999. J. Biol. Chem. 274:30468.
2. Grossman WJ, et al. 2004. Blood 104:2840.
3. Heusel JW, et al. 1994. Cell 76:977.
4. Schmid J and Weissmann C. 1987. J. Immunol. 139:250.
5. Trapani JA, et al. 1988. Proc. Natl. Acad. Sci. USA 85:6924.
6. Hiebert PR and Granville DJ. 2012. Trends Mol. Med. 18:732.
7. Saito Y, et al. 2011. J. Cardiol. 57:141.
8. Ewen CL, et al. 2012. Cell Death Differ. 1:28. - Gene ID
- 3002 View all products for this Gene ID
- UniProt
- View information about Granzyme B on UniProt.org
Other Formats
View All Granzyme B Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Granzyme B | M3304B06 | WB,IHC-P,SB |
| TotalSeq™-Bn1337 anti-Granzyme B | M3304B06 | SB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Granzyme B
Western blot analysis of PBMC (lane 1), PBMC activated with ... 
IHC staining of purified anti-Granzyme B antibody (clone M33... 
SeqIF™ (sequential immunofluorescence) staining on COMET™ of... -
TotalSeq™-Bn1337 anti-Granzyme B