- Clone
- 10D1-J16 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Chemokine (C-X-C motif) receptor 7, RDC1, CMKOR1, GPR159, ACKR3
- Isotype
- Mouse IgG2a, κ
- Barcode Sequence
- GAATTTCCCTTTGTC
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 391409 | 10 µg | $369.00 | |||
CXCR7, also known as RDC1, belongs to a subgroup of C-X-C chemokine receptors, which are part of a large protein family of G protein-coupled receptors (GPCR). CXCR7 binds with a high-affinity to CXCL12/SDF-1 and CXCL11/I-TAC, which regulates the trafficking and activation of leukocytes. It is also a co-receptor for the entry of HIV-1. The binding of a ligand to CXCR7 induces proliferation and migration of immature neurons, glia, and their precursors. CXCR7 expression occurs on a wide variety of tissues and cells including monocytes, B cells, T cells, and mature dendritic cells. Surface expression of CXCR7 has been also reported in tumor cells, activated endothelial cells, and fetal liver cells.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA
- Preparation
- The antibody was purified by chromatography and conjugated with TotalSeq™-C oligomer under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
PG - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-C antibodies are compatible with 10x Genomics Chromium Single Cell Immune Profiling Solution.
To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.
Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform. - Additional Product Notes
-
TotalSeq™ reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.
The barcode flanking sequences are CGGAGATGTGTATAAGAGACAGNNNNNNNNNN (PCR handle), and NNNNNNNNNCCCATATAAGA*A*A (capture sequence). N represents either randomly selected A, C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.
View more applications data for this product in our Scientific Poster Library. - RRID
-
AB_3106229 (BioLegend Cat. No. 391409)
Antigen Details
- Structure
- G Protein-Coupled Receptor super family
- Distribution
-
Monocytes, B cells, T cells, and mature dendritic cells.
- Function
- Regulates trafficking and activation of leukocytes.
- Ligand/Receptor
- CXCL12/SDF-1 and CXCL11/I-TAC.
- Cell Type
- B cells, Dendritic cells, Monocytes, T cells
- Biology Area
- Cell Biology, Immunology, Signal Transduction
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, GPCR
- Antigen References
-
1. Torossian F, et al. 2014. Blood 123:191.
2. Walentowicz-Sadlecka M, et al. 2014. PLoS One 1:e84629.
3. Yates TJ, et al. 2013. Cancer 119:61.
4. Burns JM, et al. 2006. J. Exp. Med. 203:2201.
5. Infantino S, et al. 2006. J. Immunol. 176:2197. - Gene ID
- 57007 View all products for this Gene ID
- UniProt
- View information about CXCR7 on UniProt.org
Other Formats
View All CXCR7 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CXCR7 | 10D1-J16 | FC |
| PE anti-human CXCR7 | 10D1-J16 | FC |
| APC anti-human CXCR7 | 10D1-J16 | FC |
| TotalSeq™-B1294 anti-human CXCR7 | 10D1-J16 | PG |
| TotalSeq™-C1294 anti-human CXCR7 | 10D1-J16 | PG |
| TotalSeq™-A1294 anti-human CXCR7 | 10D1-J16 | PG |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-human CXCR7
CXCR7-transfected L1.2 cells were stained with purified CXCR... -
PE anti-human CXCR7
CXCR7 transfected L1.2 cells were stained with CXCR7 (clone ... -
APC anti-human CXCR7
CXCR7 transfected L1.2 cells were stained with CXCR7 (clone ... -
TotalSeq™-B1294 anti-human CXCR7
-
TotalSeq™-C1294 anti-human CXCR7
-
TotalSeq™-A1294 anti-human CXCR7