TotalSeq™-D0056 anti-human CD269 (BCMA) Antibody

Pricing & Availability
Clone
19F2 (See other available formats)
Regulatory Status
RUO
Other Names
BCM, TNFRSF17
Isotype
Mouse IgG2a, κ
Barcode Sequence
CAGATGATCCACCAT
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Cat # Size Price Quantity Check Availability
357539 10 µg $369.00
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Description

CD269, also known as B cell maturation antigen (BCMA), is a 27 kD, single pass transmembrane protein with one TNFR-Cys repeat on its extracellular domain. CD269 is a B cell maturation factor, essential for the long-term survival of plasma cells. It is expressed by plasmablasts, plasma cells, and germinal center B cells. The ligands of CD269 are BAFF and APRIL, and its cytoplasmic domain binds several of the TRAF family members.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
BCMA-mouse IgG Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA
Preparation
The antibody was purified by chromatography and conjugated with TotalSeq™-D oligomer under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

PG - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-D antibodies are compatible with Mission Bio’s Tapestri Single-Cell Sequencing Platform for simultaneous detection of DNA and Protein.

To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.


Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.
Additional Product Notes

TotalSeq™-D reagents are designed to profile protein expression at single cell level. The Mission Bio Tapestri platform and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq/single-cell-dna

The barcode flanking sequences are CGAGATGACTACGCTACTCATGG (PCR handle), and GAGCCGATCTAGTATCTCAGT*C*G (capture sequence). * indicates a phosphorothioated bond, to prevent nuclease degradation.

View more applications data for this product in our Application Technical Notes.

RRID
AB_3662390 (BioLegend Cat. No. 357539)

Antigen Details

Structure
Single pass transmembrane protein, member of the TNFR superfamily, 1 TNFR-Cys domain, 27 kD
Distribution

Plasmablasts, plasma cells, germinal center B cells

Function
B cell maturation factor, essential for survival of plasma cells
Interaction
Associates with several TRAF family members
Ligand/Receptor
BAFF, APRIL
Cell Type
B cells, Plasma cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Coquery CM and Erickson LD. 2012. Crit. Rev. Immunol. 32:287. 
2. Notas G, et al. 2012. J. Immunol. 189:4748. 
3. Rickert RC, et al. 2011. Immunol. Rev. 244:115. 
4. Mesin L, et al. 2011. J. Immunol. 187:2867.

Gene ID
608 View all products for this Gene ID
UniProt
View information about CD269 on UniProt.org
Go To Top Version: 1    Revision Date: 06/26/2024

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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