TotalSeq™-C1266 anti-mouse CD267 (TACI) Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Rat RBL transfected cell line

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA

Preparation

The antibody was purified by chromatography and conjugated with TotalSeq™-C oligomer under optimal conditions.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

PG - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-C antibodies are compatible with 10x Genomics Chromium Single Cell Immune Profiling Solution.

To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.

Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.

Additional Product Notes

TotalSeq™ reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.

The barcode flanking sequences are CGGAGATGTGTATAAGAGACAGNNNNNNNNNN (PCR handle), and NNNNNNNNNCCCATATAAGA*A*A (capture sequence). N represents either randomly selected A, C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.

View more applications data for this product in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)

1. Siegel RM, et al. 2001. Nat. Immunol. 2:577.
2. Von Bulow GU, et al. 1997. Science 278:138.
3. Yan M, et al. 2001. Nat. Immunol. 2:638.
4. Diaz-de-Durana Y, et al. 2006. Blood 107:594.
5. Moir S, et al. 2012. Blood. 120:4850. PubMed.

RRID

AB_2941418 (BioLegend Cat. No. 133405)

Antigen Details

Structure

TACI/CD267 is a type III transmembrane protein belonging to TNF receptor superfamily (TNFSF). Among TNFSF members, TACI shares a high degree of homology with B cell maturation antigen (BCMA). It binds to the ligands BAFF (BLyS) and APRIL.

Distribution

CD267 is expressed predominantly by splenic transitional type 2 and marginal zone B cells, as well as activated B cells. But it is notably absent from germinal center B cells.

Function

CD267 appears to be particularly important for the regulation of B cell survival and maturation in the spleen. It is a major mediator of BAFF-dependent costimulatory responses in peripheral B cells. Signaling through TACI may serve to down-regulate B cell activity.

Ligand/Receptor

BAFF (Blys/TALL-1/THANK) and APRIL

Cell Type

B cells

Biology Area

Costimulatory Molecules, Immunology

Molecular Family

CD Molecules

Antigen References

1. Ng LG, et al. 2004. J. Immunology. 173:807.

Gene ID

57916 View all products for this Gene ID

UniProt

View information about CD267 on UniProt.org

Related Protocols

• TotalSeq™-B or -C with 10x Feature Barcoding Technology

Other Formats