Ultra-LEAF™ Purified anti-human GM-CSF Antibody

Pricing & Availability
Clone
BVD2-21C11 (See other available formats)
Regulatory Status
RUO
Other Names
Granulocyte/macrophage colony-stimulating factor, CSF-α, Pluripoietin-α, Eosinophil colony stimulating factor (Eo-CSF), Burst promoting activity (BPA)
Isotype
Rat IgG2a, κ
BVD2-21C11_ULEAF_GM-CSF_Antibody_1_091119_updated
Recombinant human GM-CSF (Black Circles, Cat. No. 572904) induces the proliferation of human erythroleukemia TF-1 cells. Anti-human GM-CSF antibody (Clone BVD2-21C11, Purple Squares) neutralizes the proliferation function of recombinant human GM-CSF at 0.3 ng/mL in TF-1 cells in a dose dependent manner while the isotype control (Cat. No. 400562, Green Triangles) does not have the effect. The ND50 = 0.1 - 0.8 µg/mL.
  • BVD2-21C11_ULEAF_GM-CSF_Antibody_1_091119_updated
    Recombinant human GM-CSF (Black Circles, Cat. No. 572904) induces the proliferation of human erythroleukemia TF-1 cells. Anti-human GM-CSF antibody (Clone BVD2-21C11, Purple Squares) neutralizes the proliferation function of recombinant human GM-CSF at 0.3 ng/mL in TF-1 cells in a dose dependent manner while the isotype control (Cat. No. 400562, Green Triangles) does not have the effect. The ND50 = 0.1 - 0.8 µg/mL.
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502319 100 µg $270.00
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502320 1 mg $715.00
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Description

Granulocyte/macrophage - colony stimulating factor (GM-CSF) is a hematopoietic factor that is produced by activated T cells, B cells, mast cells, macrophages, fibroblasts, and endothelial cells. In addition to supporting colony formation of granulocyte/macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli-expressed, recombinant human GM-CSF.
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by neutralization of the proliferation induced by recombinant human GM-CSF (0.3 ng/mL) on TF-1 human erythroleukemia cells. ND50 range: 0.1 - 0.8 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: ELISA1-4 or ELISPOT3,4 Detection, Neutralization1, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated5,6 and acetone-fixed7 frozen tissue sections, and immunocytochemistry.

Note: For testing human GM-CSF in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 432001 to 432006) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA, Neut, IP)
  2. Abrams J, et al. 1994. Eosinophils in Allergy and Inflammation. Marcel Dekker New York. p.133. (ELISA)
  3. Bacchetta R, et al. 1990. J. Immunol. 144:902. (ELISA)
  4. Kita H, et al. 1991. J. Exp. Med. 174:745. (ELISA)
  5. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag. (IHC)
  6. Andersson J, et al. 1994. Immunology 83:16. (IHC)
  7. Rasouli J, et al. 2015. J. Immunol. 11:5085-93. (IHC)
Product Citations
  1. Xu P, et al. 2020. Cancer Immunol Res. 8:1193. PubMed
RRID
AB_2814394 (BioLegend Cat. No. 502319)
AB_2814394 (BioLegend Cat. No. 502320)

Antigen Details

Structure
Cytokine; 22 kD (Mammalian)
Bioactivity
Growth/development factor for granulocyte/macrophage progenitors; differentiates myeloblasts/monoblasts; synergizes with Epo proliferation of erythroid/megakaryocytic progenitors
Cell Sources
T cells, macrophages, fibroblasts, endothelial cells, mast cells
Cell Targets
Granulocyte/macrophage/erythroid/megakaryocytic progenitors, myeloblasts, monoblasts
Receptors
Heterodimer GM-CSFR α subunit (CDw116); β-subunit (CDw131), which is also shared with the IL-3 and IL-5 receptor α chains
Biology Area
Cell Biology, Stem Cells
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Demetri G, et al. 1991. Blood 78:2791.
3. Fan D, et al. 1991. In vivo 5:571.
4. Negrin R, et al. 1992. Adv. Pharmacol. 23:263.

Regulation
Synergistic with IL-1, IL-3, G-CSF; E21R competitive antagonist for receptor binding; stored in ECM with heparan sulfate proteoglycans
Gene ID
1437 View all products for this Gene ID
UniProt
View information about GM-CSF on UniProt.org

Other Formats

View All GM-CSF Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-human GM-CSF BVD2-21C11 ELISA Detection,ELISPOT Detection,ICFC
PE anti-human GM-CSF BVD2-21C11 ICFC
Purified anti-human GM-CSF BVD2-21C11 ELISA Capture,ICFC,IP,WB
PerCP/Cyanine5.5 anti-human GM-CSF BVD2-21C11 ICFC
APC anti-human GM-CSF BVD2-21C11 ICFC
Pacific Blue™ anti-human GM-CSF BVD2-21C11 ICFC
Purified anti-human GM-CSF (Maxpar® Ready) BVD2-21C11 ELISA Capture,CyTOF®
PE/Dazzle™ 594 anti-human GM-CSF BVD2-21C11 ICFC
Ultra-LEAF™ Purified anti-human GM-CSF BVD2-21C11 Neut
TotalSeq™-B0943 anti-human GM-CSF BVD2-21C11 ICPG
Brilliant Violet 421™ anti-human GM-CSF BVD2-21C11 ICFC
Go To Top Version: 1    Revision Date: 09/03/2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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