- Clone
- IFNb/A1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interferon-β, Fibroblast interferon (Fi-IFN, F-IFN), Type-1 interferon, acid-stable interferon, R1-GI factor, IFN-b, IFN-beta
- Isotype
- Mouse IgG1, κ
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Recombinant human IFN-β (50 ng, lane 1) and recombinant mouse IFN-β (50 ng, lane 2) were resolved by electrophoresis (4-12% Tris-Glycine gel), transferred to nitrocellulose, and probed with LEAF™ purified anti-human IFN-β (clone IFNb/A1) antibody. Proteins were visualized using a Goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 514005 | 100 µg | $237.00 | |||
| 514006 | 1 mg | $688.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
The IFNb/A1 clone recognizes natural human IFN-β and recombinants β1a and β1b.It is capable of neutralizing IFN-β.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant HuIFN-β1b
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
WB - Quality tested
ELISA, Neut - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 mL antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Neutralization1: The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IFN-ß bioactivity (Cat. No. 514003).
Additional reported applications (for the relevant formats) include: ELISA1.
Epitope: Human IFN-β site I, core peptide amino acid residues 32-56, AB loop (AB2, AB3) -
Application References
(PubMed link indicates BioLegend citation) -
- Redlich PN and Grossberg SE, et al. 1989. J. Immunol. 143:1887. (ELISA, Neut)
- Redlich PN, et al. 1991. P. Natl. Acad. Sci. USA 88:4040.
- Runkel L, et al. 2001. IFN & Cytokine Res. 21:931.
- RRID
-
AB_2814441 (BioLegend Cat. No. 514005)
AB_2814441 (BioLegend Cat. No. 514006)
Antigen Details
- Structure
- Interferon; contains 187 amino acids with predicted molecular weight 22 kD.
- Bioactivity
- Non-specific humoral immune responses and immune responses against viral infections, increases expression of MHC class I antigens, blocks expression of MHC class II antigens stimulated by IFN-γ
- Cell Sources
- Fibroblasts, epithelial cells
- Cell Targets
- Broad, includes NK cells, T suppressor cells, activated monocytes
- Receptors
- IFNα/βR (IFNAR-1, IFNAR-2)
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Signal Transduction
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Nagarajan U, 2011. Crit. Rev. Immunol. 31:459.
2. Honda K, et al. 2005. Int. Immunol. 17:1367. - Regulation
- Upregulated by viruses, double-stranded RNA, micro-organisms, TNF, IL-1
- Gene ID
- 3456 View all products for this Gene ID
- UniProt
- View information about IFN-beta on UniProt.org
Other Formats
View All IFN-β Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human IFN-β | IFNb/A1 | WB,ELISA,Neut |
| Ultra-LEAF™ Purified anti-human IFN-β | IFNb/A1 | WB,ELISA,Neut |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human IFN-β
Recombinant human IFN-β (50 ng, lane 1) and recombinant mous... -
Ultra-LEAF™ Purified anti-human IFN-β
Recombinant human IFN-β (50 ng, lane 1) and recombinant mous...