Ultra-LEAF™ Purified anti-mouse CD11a Antibody

Pricing & Availability
Clone
M17/4 (See other available formats)
Regulatory Status
RUO
Other Names
αL integrin, LFA-1 α subunit, Ly-15, Ly-21, ITGAL
Isotype
Rat IgG2a, κ
M17slash4_LEAF_042706
C57BL/6 mouse splenocytes stained with Ultra-LEAF™ purified CD11a (clone M17/4), followed by anti-rat IgGs FITC.
  • M17slash4_LEAF_042706
    C57BL/6 mouse splenocytes stained with Ultra-LEAF™ purified CD11a (clone M17/4), followed by anti-rat IgGs FITC.
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Cat # Size Price Quantity Check Availability
101117 100 µg $110.00
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101118 1 mg $149.00
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101127 5 mg $539.00
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101128 25 mg $1408.00
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101129 50 mg $2354.00
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101130 100 mg $3383.00
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

CD11a is a 180 kD glycoprotein, also known as αL integrin, LFA-1 α, Ly-15, or Ly-21. It is a member of the integrin family, primarily expressed on lymphocytes, monocytes/macrophages, and granulocytes. In association with CD18, the CD11a/CD18 complex forms LFA-1. CD11a plays an important role in intercellular adhesion and costimulation by binding its ligands, ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50).

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C57BL/6 mouse splenic secondary cytotoxic T cells
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume or 100 µl of whole blood.  It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The M17/4 antibody can block CD11a-mediated cellular adhesion. Additional reported applications of this antibody (for the relevant formats) include: immunoprecipitation1,2, in vitro blocking of cell-cell adhesion1,2 and FOXP3 expression5, and immunohistochemical staining of acetone-fixed frozen sections3. The M17/4 antibody does not block the binding of 2D7 antibody (Cat. No. 101002) to CD11a. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 101110). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 101118) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Sanchez-Madrid F, et al. 1982. Cell Immunol. 73:1. (IP, Block)
  2. Kuhlman P, et al. 1991. J. Immunol. 146:1773. (IP, Block)
  3. Mizgerd JP, et al. 1997. J. Exp. Med. 186:1357. (IHC)
  4. Hailman E and Allen PM. 2005. J. Immunol. 175:4847. (FC)
  5. Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (Block) PubMed
Product Citations
  1. Bae SH, et al. 2021. Theranostics. 11:2522. PubMed
  2. Wolf D, et al. 2018. Nat Commun. 9:525. PubMed
  3. Waldman MM, et al. 2022. Front Immunol. 13:856977. PubMed
RRID
AB_2561478 (BioLegend Cat. No. 101117)
AB_2561478 (BioLegend Cat. No. 101118)
AB_2561478 (BioLegend Cat. No. 101127)
AB_2561478 (BioLegend Cat. No. 101128)
AB_2561478 (BioLegend Cat. No. 101129)
AB_2561478 (BioLegend Cat. No. 101130)

Antigen Details

Structure
Integrin family, associates with integrin β2 (CD18), 180 kD
Distribution

Lymphocytes, granulocytes, monocytes, macrophages

Function
Cellular adhesion
Ligand/Receptor
ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50)
Cell Type
Granulocytes, Lymphocytes, Macrophages, Monocytes, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lub M, et al. 1995. Immunol. Today 16:479.

Gene ID
16408 View all products for this Gene ID
UniProt
View information about CD11a on UniProt.org
Go To Top Version: 2    Revision Date: 08/20/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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