- Clone
- 17B5 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- 4-1BB, TNFRSF9, Ly-63, ILA (Induced by Lymphocyte Activation), CD137
- Isotype
- Syrian Hamster IgG
-
Con A-stimulated BALB/c mouse splenocytes stained with Ultra-LEAF™ purified 17B5, followed by biotinylated anti-Syrian hamster IgG and Sav-PE
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 106113 | 100 µg | $110.00 | |||
| 106114 | 1 mg | $149.00 | |||
| 106115 | 5 mg | $539.00 | |||
| 106116 | 25 mg | $1408.00 | |||
| 106117 | 50 mg | $2354.00 | |||
| 106118 | 100 mg | $3383.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD137 is a 39 kD TNF-receptor superfamily member also known as 4-1BB, TNFRSF9, Ly-63, and ILA (Induced by Lymphocyte Activation). CD137 is expressed as monomers, dimers, and tetramers on activated T and B cells. Interaction of CD137 with its ligand, 4-1BBL (CD137L), is important in T-B cell costimulation. The 17B5 antibody has been reported to block 4-1BBL costimulated T cell proliferation.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Syrian Hamster
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Block - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: in vitro blocking of 4-1BB-mediated T cell proliferation2. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 106108).
-
Application References
(PubMed link indicates BioLegend citation) - Product Citations
-
- RRID
-
AB_2832348 (BioLegend Cat. No. 106113)
AB_2832348 (BioLegend Cat. No. 106114)
AB_2832348 (BioLegend Cat. No. 106115)
AB_2832348 (BioLegend Cat. No. 106116)
AB_2832348 (BioLegend Cat. No. 106117)
AB_2832348 (BioLegend Cat. No. 106118)
Antigen Details
- Structure
- TNF-R superfamily, 39 kD
- Distribution
-
Activated T cells and B cells
- Function
- T-B cell costimulation
- Ligand/Receptor
- 4-1BB ligand (CDw137L)
- Cell Type
- B cells, T cells, Tregs
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Alderson MR, et al. 1994. Eur. J. Immunol. 24:2219.
3. Schwarz H, et al. 1996. Blood 87:2839. - Gene ID
- 21942 View all products for this Gene ID
- UniProt
- View information about CD137 on UniProt.org
Other Formats
View All CD137 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Biotin anti-mouse CD137 | 17B5 | FC |
| PE anti-mouse CD137 | 17B5 | FC |
| APC anti-mouse CD137 | 17B5 | FC |
| TotalSeq™-A0194 anti-mouse CD137 | 17B5 | PG |
| Ultra-LEAF™ Purified anti-mouse CD137 | 17B5 | FC,Block |
| TotalSeq™-C0194 anti-mouse CD137 | 17B5 | PG |
| TotalSeq™-B0194 anti-mouse CD137 | 17B5 | PG |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Biotin anti-mouse CD137
Con A-stimulated BALB/c mouse splenocytes stained with bioti... -
PE anti-mouse CD137
Con A-stimulated (3 days) splenocytes stained with 17B5 PE -
APC anti-mouse CD137
ConA+IL-2 (3 days) stimulated C57BL/6 splenocytes were stain... -
TotalSeq™-A0194 anti-mouse CD137
-
Ultra-LEAF™ Purified anti-mouse CD137
Con A-stimulated BALB/c mouse splenocytes stained with Ultra... -
TotalSeq™-C0194 anti-mouse CD137
-
TotalSeq™-B0194 anti-mouse CD137