- Clone
- TH6 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Folate receptor 4, FBP3, FR δ
- Isotype
- Rat IgG2b, κ
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C57BL/6 mouse splenocytes stained with CD3 (145-2C11) APC and Ultra-LEAF™ purified TH6, followed by anti-rat IgG PE.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 125111 | 100 µg | $270.00 | |||
| 125112 | 1 mg | $743.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
Folate receptor 4 (FR4) is a surface receptor for folic acid (Vitamin B9).It was recently reported that FR4 is highly and constitutively expressed on mouse CD4+ CD25+ natural regulatory T cells (Treg).The high expression of this surface marker on Treg can be used in combination with CD4 and CD25 to distinguish Treg from other types of T cells.Since FR4 is a surface marker,this antibody may be used in combination with anti-CD4 and/or CD25 to sort living T reg cells for functional studies.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Cyt, Depletion - Reported in the Literature - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
-
Application References
(PubMed link indicates BioLegend citation) -
1. Yamaguchi T et al. 1007: Immunity. 27(1)145
2. Inoue N, et al. 2015. Nat Commun. 6: 8858. PubMed
3. Kato K, et al. 2016. Nat Commun. 7:12198. PubMed - RRID
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AB_2890682 (BioLegend Cat. No. 125111)
AB_2890682 (BioLegend Cat. No. 125112)
Antigen Details
- Structure
- 28 kd protein consisting of 244 amino acids. Heavily glycosylated
- Distribution
-
Expressed on mouse thymocytes and spleen cells. Highly expressed on natural regulatory (Treg) cells
- Function
- Mediate calcium-independent homophlic cell-cell adhesion
- Ligand/Receptor
- Folic acid and its derivative
- Cell Type
- Thymocytes, Tregs
- Biology Area
- Immunology, Innate Immunity
- Antigen References
-
1. Yamaguchi T, et al. 2007. Immunity. 27:145
2. Spiegelstein O, et al. 2000. Gene. 258:117 - Gene ID
- 64931 View all products for this Gene ID
- UniProt
- View information about FR4 on UniProt.org
Other Formats
View All Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Biotin anti-mouse FR4 (Folate Receptor 4) | TH6 | FC |
| PE anti-mouse FR4 (Folate Receptor 4) | TH6 | FC |
| TotalSeq™-C1058 anti-mouse FR4 (Folate Receptor 4) | TH6 | PG |
| Ultra-LEAF™ Purified anti-mouse FR4 (Folate Receptor 4) Antibody | TH6 | FC,Cyt,Depletion |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Biotin anti-mouse FR4 (Folate Receptor 4)
BALB/c mouse splenocytes stained with CD3 (145-2C11) APC and... -
PE anti-mouse FR4 (Folate Receptor 4)
BALB/c mouse splenocytes stained with TH6 PE and CD3 (145-2C... -
TotalSeq™-C1058 anti-mouse FR4 (Folate Receptor 4)
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Ultra-LEAF™ Purified anti-mouse FR4 (Folate Receptor 4) Antibody
C57BL/6 mouse splenocytes stained with CD3 (145-2C11) APC an...