Ultra-LEAF Purified anti-mouse IL-10 Antibody, IL-10, JES5-16E3

Pricing & Availability

Clone: JES5-16E3 (See other available formats)
Regulatory Status: RUO
Other Names: Interleukin-10, Cytokine synthesis inhibitory factor (CSIF), B cell derived T cell growth factor (B-TCGF)
Isotype: Rat IgG2b, κ

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Cat #

Size

Price

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505037

100 µg

$182.00

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505038

1 mg

$633.00

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Description

IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. IL-10 inhibits IFN-γ, TNF-β, and IL-2 production by Th1 clones; inhibits macrophage-mediated IL-1, IL-6, and TNF-α synthesis; suppresses the delayed type hypersensitivity response; stimulates Th2 cell response (which results in elevated antibody production); and promotes mast cell proliferation in combination with IL-4.

Technical data sheet

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

E. coli-expressed, recombinant mouse IL-10

Formulation

0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.

Preparation

The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.

Application

ELISA Capture - Quality tested
CyTOF® - Verified
ELISPOT Capture, Neut, ICFC, IHC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.1 - 1.0 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. To obtain a linear standard curve, serial dilutions of IL-10 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. For ELISA capture, Carbonate buffer, pH 9.5 is highly recommended for coating buffer.

Application Notes

ELISA or ELISPOT Detection^1,9,11: The biotinylated JES5-16E3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified JES5-2A5 antibody (Cat. Nos. 504902 & 504904) as the capture antibody.
ELISA Capture: The purified JES5-16E3 antibody is useful as the capture antibody in a sandwich ELISA when used in conjunction with the biotinylated JES5-2A5 antibody (Cat. Nos. 504905 & 504906) as the detection antibody and recombinant mouse IL-10 (Cat. No. 575809) as the standard.
Neutralization¹⁴: The Ultra-LEAF™ purified JES5-16E3 antibody can neutralize the bioactivity of natural or recombinant IL-10.
Flow Cytometry³: The fluorochrome-labeled JES5-16E3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-10-producing cells within mixed cell populations.
Additional reported applications (for relevant formats) include: immunohistochemistry³.

Application References (PubMed link indicates BioLegend citation)

Simkin G, et al. 2000. J. Immunol. 164:2457.
Kitagaki K, et al. 2002. Clin. Diagn. Lab Immunol. 9:1260.
Khanna A, et al. 2000. J. Immunol. 164:1346.
Sander B, et al. 1993. J. Immunol. Methods 166:201.
Litton M, et al. 1994. J. Immunol. Methods 175:47.
Andersson U, et al. 1999. Detection and qunatification of gene expression. New York:Springer-Verlag.
Finkelman F, et al. 2003. Curr. Prot. Immunol. John Wiley & Sons New York. Unit 6.28.
Wang W, et al. 2004. FASEB J. 18:1043.
Brummel R and Lenert P. 2005. J. Immunol. 174:2429.
Lawson BR, et al. 2007. J. Immunol. 178:5366.
Xu G, et al. 2007. J. Immunol. 179:5358. PubMed
Brummel R, et al. 2005. J. Immunol.174:2429. PubMed
Kang YJ, et al. 2007. Stem Cells 25:1814. PubMed
Seo N, et al. 2001. Immunology. 103:449. (Neut)

RRID

AB_2814423 (BioLegend Cat. No. 505037)
AB_2814423 (BioLegend Cat. No. 505038)

Antigen Details

Structure: Acid-labile cytokine, dimer, 17-21 kD (Mammalian)
Cell Sources: Activated CD8⁺ T cells, Th0, Th2 subset of CD4⁺ T cells, Ly-1⁺ B cells, monocytes, macrophages, keratinocytes
Cell Targets: T cells, B cells, mast cells, macrophages
Receptors: IL-10R (CDw210)
Cell Type: Tregs
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. de Waal-Malefy R, et al. 1992. Curr. Opin. Immunol. 4:314.
3. Howard M, et al. 1992. Immunol. Today 13:198.
4. Quesniaux V. 1992. Res. Immunol. 143:385.
5. Norton SK, et al. 2008. J. Immunol. 180:2848.
Regulation: Downregulated by IL-4, IL-10
Gene ID: 16153 View all products for this Gene ID
UniProt: View information about IL-10 on UniProt.org

Related Protocols

Related Products

Description	Clone	Applications

Other Formats

View All IL-10 Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-mouse IL-10	JES5-16E3	ICFC
Biotin anti-mouse IL-10	JES5-16E3	ELISA Detection,ELISPOT Detection,ICFC
FITC anti-mouse IL-10	JES5-16E3	ICFC
PE anti-mouse IL-10	JES5-16E3	ICFC
Purified anti-mouse IL-10	JES5-16E3	ELISA Capture,CyTOF®,IHC
Alexa Fluor® 647 anti-mouse IL-10	JES5-16E3	ICFC
PE/Cyanine7 anti-mouse IL-10	JES5-16E3	ICFC
Alexa Fluor® 488 anti-mouse IL-10	JES5-16E3	ICFC
Brilliant Violet 421™ anti-mouse IL-10	JES5-16E3	ICFC
Pacific Blue™ anti-mouse IL-10	JES5-16E3	ICFC
PerCP/Cyanine5.5 anti-mouse IL-10	JES5-16E3	ICFC
Purified anti-mouse IL-10 (Maxpar® Ready)	JES5-16E3	ELISA Capture,CyTOF®
Brilliant Violet 605™ anti-mouse IL-10	JES5-16E3	ICFC
PE/Dazzle™ 594 anti-mouse IL-10	JES5-16E3	ICFC
APC/Cyanine7 anti-mouse IL-10	JES5-16E3	ICFC
Ultra-LEAF™ Purified anti-mouse IL-10	JES5-16E3	ELISA Capture,CyTOF®,ELISPOT Capture,Neut,ICFC,IHC
Brilliant Violet 650™ anti-mouse IL-10	JES5-16E3	ICFC
Brilliant Violet 711™ anti-mouse IL-10	JES5-16E3	ICFC

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