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Alexa Fluor® 594 anti-His Tag Antibody

Pricing & Availability
Clone
J095G46 (See other available formats)
Regulatory Status
RUO
Other Names
Hexa histidine-tag, 6xHis-tag, 6XHis, 6His
Isotype
Mouse IgG2a, κ
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Product Citations
publications
J095G46_A594_His-Tag_IF_1_092717
HeLa cells transiently transfected with His Tag fused protein were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 1:2500 diluted (0.2 µg/ml) Alexa Fluor® 594 anti-His tag antibody (red). Nuclei were counterstained with DAPI (blue, Cat. No. 422801). The image was captured with a 40X objective.
  • J095G46_A594_His-Tag_IF_1_092717
    HeLa cells transiently transfected with His Tag fused protein were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 1:2500 diluted (0.2 µg/ml) Alexa Fluor® 594 anti-His tag antibody (red). Nuclei were counterstained with DAPI (blue, Cat. No. 422801). The image was captured with a 40X objective.
  • J095G46_A594_His-Tag_IF_2_092717
    HeLa cells transiently transfected with His Tag fused protein or non-transfected were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 1:250 (2 µg/ml), 1: 1000 (0.5 µg/ml) and 1:2500 diluted (0.2 µg/ml) Alexa Fluor® 594 anti-His Tag antibody (red). Nuclei were counterstained with DAPI (blue, Cat. No. 422801). The image was captured with a 40X objective using KEYENCE BZ-X700 fluorescence microscope.
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362609 25 µg $159
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Description

This antibody was raised against a linear 8XHis tag. It reacts with His-tagged protein with C, N or internal 6XHis tags or longer. It can be used for western blotting to detect linear His repeats (6XHis or more). It can also be used for ICFC and ICC staining of His-tagged proteins.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat, All species
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH-conjugated His-tag peptide.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1:250-1:2500 (0.2-2ug/mL) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

This antibody was raised against a linear 8X His tag. It reacts with proteins displaying repeats of 6 Histidines (His6) or more in either internal or C and N terminal regions. It is suitable for ICFC.

Clone J095G46 has been verified for immunocytochemistry (ICC).

RRID
AB_2716214 (BioLegend Cat. No. 362609)

Antigen Details

Structure
6 repeat His amino acid
Function
Epitope tag
Biology Area
Cell Biology, Immunology
Antigen References
  1. Zhao C, et al. 2010. Anal Biochem. 399:237
  2. Salichs E, et al. 2009. PLoS Genet. 5:e1000397
  3. Terpe K. 2003. Appl. Microbiol. Biotechnol. 60:523
  4. Lindner, P, et al. 1997. Biotechniques. 22:140
  5. Burritt JB, et al. 1995. J. Biol. Chem. 270:16974.
  6. Taylor RM, et al. 2007. Methods Mol. Biol. 412:429.
Gene ID
NA
UniProt
View information about His Tag on UniProt.org
Go To Top Version: 1    Revision Date: 09/27/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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