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Alexa Fluor® 700 anti-human CD42b Antibody

Pricing & Availability
Clone
HIP1 (See other available formats)
Regulatory Status
RUO
Workshop
IV P70
Other Names
Glycocalicin, gpIbα
Isotype
Mouse IgG1, κ
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Product Citations
publications
HIP1_A700_CD42b_Antibody_080717
Human platelets were stained with CD42b (clone HIP1) Alexa Fluor® 700 (filled histogram) or mouse IgG1, κ Alexa Fluor® 700 isotype control (open histogram).
  • HIP1_A700_CD42b_Antibody_080717
    Human platelets were stained with CD42b (clone HIP1) Alexa Fluor® 700 (filled histogram) or mouse IgG1, κ Alexa Fluor® 700 isotype control (open histogram).
Compare all formats See Alexa Fluor® 700 spectral data
Cat # Size Price Quantity Check Availability Save
303927 25 tests $141
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303928 100 tests $358
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Description

CD42b is a 145 kD glycoprotein known as gpIbα. It is covalently bonded to CD42c to form GPIb. CD42b is expressed on platelets and megakaryocytes. CD42b/c heterodimer forms a complex with CD42a and d and acts as the receptor for von Willibrand factor and thrombin.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Clone HIP1 recognizes an epitope within the N-terminal region of the GPIba chain5. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, Westerm blotting, and inhibition of platelet aggregation2. The Ultra LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 303939).

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Takahashi R, et al. 1999. Blood. 93:1951.
  3. Saggu G, et al. 2013. J. Immunol. 190:6457. PubMed
  4. Meyer Dos Santos S, et al. 2011. Blood. 117:4999. (Block) PubMed
  5. Vettore S, et al. 2008. Haematologica. 93:1743.
Product Citations
  1. Vanuytsel K, et al. 2022. Cytometry A. 101:903. PubMed
RRID
AB_2715928 (BioLegend Cat. No. 303927)
AB_2715928 (BioLegend Cat. No. 303928)

Antigen Details

Structure
Mucin, leucine-rich repeat family, 145 kD
Distribution

Platelets, megakaryocytes

Function
Complex with CD42a, c and d, platelet adhesion and activation
Ligand/Receptor
Von Willebrand factor, thrombin
Cell Type
Megakaryocytes, Platelets
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Clemetson K, et al. 1982. J. Clin. Invest. 70:304.
2. Fox J, et al. 1988. J. Biol. Chem. 263:4882.
3. Kuijpers R, et al. 1992 Blood 79:283.

Gene ID
2812 View all products for this Gene ID
UniProt
View information about CD42b on UniProt.org
Go To Top Version: 0    Revision Date: 08/07/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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