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Brilliant Violet 421™ anti-mouse TCR β chain Antibody

Pricing & Availability
Clone
H57-597 (See other available formats)
Regulatory Status
RUO
Other Names
TCR-β chain, TCR-β, β-TCR
Isotype
Armenian Hamster IgG
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Product Citations
publications
H57-597_BV421_051311
C57BL/6 mouse splenocytes were stained with TCR β (clone H57-597) Brilliant Violet 421™ (filled histrogram) or Armenian hamster IgG Brilliant Violet 421™ isotype control (open histogram).
  • H57-597_BV421_051311
    C57BL/6 mouse splenocytes were stained with TCR β (clone H57-597) Brilliant Violet 421™ (filled histrogram) or Armenian hamster IgG Brilliant Violet 421™ isotype control (open histogram).
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
109229 125 µL $176
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109230 50 µg $242
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Description

T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR-β is a member of the immunoglobulin superfamily and a component of the CD3/TCR complex (along with TCR-α). It is expressed on α/β TCR-bearing T cells and thymocytes. The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Affinity purified TCR from mouse DO-11.10 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For immunofluorescent staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

H57-597 is a hamster mAb directed to an epitope of the C region of TCR ß chain12. The H57-597 antibody does not cross-react with ?/d TCR-bearing T cells. Immobilized or soluble H57-597 antibody can activate a/ß TCR-bearing T cells. Additional reported applications (for the relevant formats) for this antibody include: immunoprecipitation2, in vitro stimulation2,3, in vivo depletion4-6, and immunohistochemical staining of acetone-fixed frozen sections7,8,9. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 109253-109258).

Application References

(PubMed link indicates BioLegend citation)
  1. Gascoigne NJ. 1990. J. Biol. Chem. 265:9296.
  2. Kruisbeek A, et al. 1991. In Current Protocols in Immunology. pp. 3.12.1. (Costim IP)
  3. Davenport C, et al. 1995. J. Immunol. 155:3742. (Costim)
  4. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  5. Kummer U, et al. 2001. Immunol. Lett. 75:153. (Deplete)
  6. van der Heyde HC, et al. 1995. J. Immunol. 154:3985. (Deplete)
  7. Tomita K, et al. 1999. Genes Dev. 13:1203. (IHC)
  8. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
  9. Ponomarev ED, et al. 2007. J. Immunol. 178:39. (IHC)
  10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
  11. Tsukumo S, et al. 2006. J.Immunol. 177:8365. (FC) PubMed
  12. Grégoire C, et al. 1991. Proc. Natl. Acad. Sci USA 88:8077.
Product Citations
  1. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
  2. Soon MSF, et al. 2020. Nat Immunol. 1.984027778. PubMed
  3. Lee SA, et al. 2020. J Immunol. 204:586. PubMed
  4. Campisi L, et al. 2022. Nature. 606:945. PubMed
  5. Glaubitz J, et al. 2023. Gut. :. PubMed
  6. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  7. Michelet X, et al. 2015. J Immunol. 194:2079. PubMed
  8. Shibata K, et al. 2022. Nat Commun. 13:6948. PubMed
  9. Donado CA, et al. 2020. Cell Reports. 31(1):107466. PubMed
  10. Park JG, et al. 2021. iScience. 24(9):102941. PubMed
  11. Yang W, et al. 2020. Nat Commun. 3.553472222. PubMed
  12. Tyagi AM et al. 2018. Immunity. 49(6):1116-1131 . PubMed
  13. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  14. Gómez-Díaz C, et al. 2021. iScience. 24:103241. PubMed
  15. Wiede F, et al. 2021. Cancer Discov. Online ahead of print. PubMed
  16. Chen S et al. 2018. Cell reports. 25(7):1729-1740 . PubMed
  17. Yu M, et al. 2021. J Clin Invest. 131:. PubMed
  18. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  19. Prosser A, et al. 2021. Cell Reports. 35(7):109141. PubMed
  20. Kawakami R, et al. 2021. Immunity. 54(5):947-961.e8. PubMed
  21. Romano A, et al. 2015. J Immunol. 195: 3816 - 3827. PubMed
  22. Sun Y, et al. 2022. Nat Commun. 13:3916. PubMed
  23. Canton J, et al. 2021. Nat Immunol. 22:140. PubMed
  24. Jackson-Jones LH, et al. 2020. Immunity. 52:700. PubMed
  25. Tyagi AM, et al. 2021. eLife. 10:00. PubMed
  26. Fennell LM, et al. 2020. EMBO J. 39:e103303. PubMed
  27. Lu X, et al. 2020. Sci Transl Med. 12:. PubMed
  28. Watanabe M, et al. 2020. Nat Commun. 4.808333333. PubMed
  29. Goh PK, et al. 2022. Sci Adv. 8:eabk3338. PubMed
  30. Nagatake T, et al. 2018. Int Immunol. 30:471. PubMed
  31. Jandke A, et al. 2020. Nat Commun. 3.075694444. PubMed
  32. Xi‐Zhi J Guo et al. 2018. Immunity. 49(3):531-544 . PubMed
  33. Xiao S, et al. 2020. Cell Reports. 32(2):107892. PubMed
  34. Peters N, et al. 2014. PLoS Pathog. 10:1004538. PubMed
  35. Nagatake T, et al. 2018. J Allergy Clin Immunol. 142:470. PubMed
  36. Oliveira AC et al. 2017. eLife. 6 pii: e30883. PubMed
  37. Wei Y, et al. 2021. Endocrinology. 162(8):. PubMed
  38. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
  39. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
  40. Muri J, et al. 2020. Cell Reports. 29(9):2731-2744.e4.. PubMed
  41. Iwanami N, et al. 2020. iScience. 23:101260. PubMed
  42. Boulch M, et al. 2021. Sci Immunol. 6:. PubMed
  43. Ito Y, et al. 2021. Cell Reports. 35(4):109052. PubMed
  44. Trivedi S, et al. 2020. Elife. 9:00. PubMed
RRID
AB_10933263 (BioLegend Cat. No. 109229)
AB_10933263 (BioLegend Cat. No. 109230)

Antigen Details

Structure
Ig superfamily, CD3/TCR complex with CD3 and TCR α subunit
Distribution

Majority of T cells and thymocytes (correlated to differentiation)

Function
Antigen recognition, T cell activation
Ligand/Receptor
Peptide bound-MHC class I and II
Antigen References

1. Davis MM, et al. 1998. Ann. Rev. Immunol. 16:523.
2. Huppa JB, et al. 2003. Nat. Immunol. 4:749.
3. Kubo R, et al. 1989. J. Immunol. 142:2736.

Gene ID
21577 View all products for this Gene ID
UniProt
View information about TCR beta chain on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Other Formats

View All TCR β chain Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse TCR β chain H57-597 FC
Biotin anti-mouse TCR β chain H57-597 FC,IHC-F
FITC anti-mouse TCR β chain H57-597 FC,IHC-F
PE anti-mouse TCR β chain H57-597 FC
PE/Cyanine5 anti-mouse TCR β chain H57-597 FC
Purified anti-mouse TCR β chain H57-597 FC,CyTOF®,IHC-F,IP,Costim,Depletion
Alexa Fluor® 488 anti-mouse TCR β chain H57-597 FC,IHC-F
Alexa Fluor® 647 anti-mouse TCR β chain H57-597 FC,IHC-F
APC/Cyanine7 anti-mouse TCR β chain H57-597 FC
PE/Cyanine7 anti-mouse TCR β chain H57-597 FC
Alexa Fluor® 700 anti-mouse TCR β chain H57-597 FC
Pacific Blue™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 421™ anti-mouse TCR β chain H57-597 FC,IHC-F
PerCP/Cyanine5.5 anti-mouse TCR β chain H57-597 FC
Brilliant Violet 570™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 510™ anti-mouse TCR β chain H57-597 FC,IHC-F
Purified anti-mouse TCR β chain (Maxpar® Ready) H57-597 FC,CyTOF®
Alexa Fluor® 594 anti-mouse TCR β chain H57-597 FC,IHC-F
PE/Dazzle™ 594 anti-mouse TCR β chain H57-597 FC
Brilliant Violet 605™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 711™ anti-mouse TCR β chain H57-597 FC
APC/Fire™ 750 anti-mouse TCR β chain H57-597 FC
TotalSeq™-A0120 anti-mouse TCR β chain H57-597 PG
Brilliant Violet 785™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 650™ anti-mouse TCR β chain H57-597 FC
Ultra-LEAF™ Purified anti-mouse TCR β chain H57-597 FC,CyTOF®,IHC-F,IP,Costim,Depletion
TotalSeq™-C0120 anti-mouse TCR β chain H57-597 PG
TotalSeq™-B0120 anti-mouse TCR β chain H57-597 PG
Spark Red™ 718 anti-mouse TCR β chain (Flexi-Fluor™) H57-597 FC
Spark PLUS UV395™ anti-mouse TCR β chain H57-597 FC
TotalSeq™-D0120 anti-mouse TCR β chain H57-597 PG
Spark Blue™ 574 anti-mouse TCR β chain (Flexi-Fluor™) H57-597 FC
Spark Blue™ 550 anti-mouse TCR β chain (Flexi-Fluor™) H57-597 FC
Go To Top Version: 2    Revision Date: 02/06/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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