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FITC Annexin V

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Regulatory Status
RUO
Other Names
Annexin A5
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AnnexinV_FITC_092908.jpg
Human T leukemia cell line, Jurkat, non-treated (top) or treated (bottom) with BioLegend’s anti-human CD95 (EOS9.1) mAb (cat. 305704) for 6 hours, then stained with Annexin V-FITC and Propidium Iodide (PI) (cat. 421301)
  • AnnexinV_FITC_092908.jpg
    Human T leukemia cell line, Jurkat, non-treated (top) or treated (bottom) with BioLegend’s anti-human CD95 (EOS9.1) mAb (cat. 305704) for 6 hours, then stained with Annexin V-FITC and Propidium Iodide (PI) (cat. 421301)
  • B_AnnexinV_FITC_092908.jpg
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640905 25 tests $83
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640906 100 tests $194
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640945 300 tests $405
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Description

Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry is lost and PS translocates to the external leaflet. Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells. Annexin V Binding Buffer (cat. no. 422201) is recommended for use with Annexin V staining.Annexin V binding alone cannot differentiate between apoptotic cells and necrotic. Therefore, we recommend using our Helix NP™ Blue (Cat. No. 425305), Helix NP™ Green (Cat.  No. 425303) or Helix NP™ NIR (Cat. No. 425301). Early apoptotic cells will exclude 7-AAD and PI, while late stage apoptotic cells and necrotic cells will stain positively, due to the passage of these dyes into the nucleus where they bind to DNA.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Reported Reactivity
Other Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The purified protein was conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The Annexin V solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this product is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per 100,000 - million cells in a 100 µl volume of Annexin V Binding Buffer (Cat No. 422201). It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Annexin V Staining

  1. Wash cells twice with cold BioLegend Cell Staining Buffer (Cat. No. 420201) and then resuspend cells in Annexin V Binding Buffer (Cat. No. 422201) at a concentration of 1x106 cells/mL.
  2. Transfer 100 µL of cell suspension in 5 mL test tube.
  3. Add 5 µL of fluorochrome conjugated Annexin V.
  4. Stain with a viability dye, such as PI (Cat. No. 421301), 7-AAD (Cat. Nos. 420403 & 420404), or Helix NP dyes (Cat. Nos. 425301, 425303, & 425305), if desired.
  5. Gently vortex the cells and incubate for 15 min at RT (25°C) in the dark.
  6. Add 400* µL of Annexin V Binding Buffer (Cat. No. 422201) to each tube. *For more concentrated samples, add a minimum of 200 µl of Annexin V Binding Buffer in this step.
  7. Analyze by flow cytometry.

For a better experience detecting apoptosis, we now recommend Apotracker™. Cell staining with Apotracker™ is Calcium independent. Thus, no special buffers are required, and the protocol can be shortened for single-step co-staining with other reagents.

Application References

(PubMed link indicates BioLegend citation)
  1. Koopman G, et al. 1994. Blood 84:1415.
  2. Vermes I, et al. 1995. J. Immunol. Methods 184:39.
  3. Dachary-Prigent J, et al. 1993. Blood 81:2554.
  4. Sekine C, et al. 2009. Int Immunol. PubMed
  5. Grujic M, et al. 2010. J. Immunol. 185:1730. PubMed
  6. Mulik RS, et al. 2010. Int J Pharm. 398:190. PubMed
  7. Gupta A, et al. 2011. Leuk Res. 35:1498. PubMed
  8. Speth C,et al. 2013. J Infect Dis. 207:823. PubMed
  9. Wang Q, et al. 2013. Genes Dev. 27:615. PubMed
  10. Gill K, et al. 2013. Biocheim Biophys Acta. 830:2763. PubMed
  11. Juel HB, et al. 2013. PLoS One. 8:64619. PubMed
  12. Hellmann J, et al. 2013. J. Immunol. 191:1383. PubMed
  13. Sequier S, et al. 2013. PLoS One. 8:70937. PubMed
  14. Galski H, et al. 2013. Biochem Pharmacol. 86:584. PubMed
  15. Chen YL, et al. 2013. J Exp Med. PubMed
  16. Lin WH, et al. 2014. PLoS One. 9:83160. PubMed
  17. Salge-Bartles U, 2014. Thromb res. 133:244. PubMed
  18. Zhang X, et al. 2014. Acta Biochim Biophys Sin. 46:261. PubMed
  19. Intermann J, et al. 2014. Cell Signal. 26:1567. PubMed
  20. de Vires M, 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
  21. M Parrula MC, et al. 2014. Virus Res. 189:206. PubMed
  22. Passaro C, et al. 2014. Mol Oncol. 9:78. PubMed
  23. Tsou WI, et al. 2014. J Biol Chem. 289:25750. PubMed
  24. Awojoodu AO, et al. 2014. Blood. 124:1941. PubMed
  25. Xiong R, et al. 2014. Toxicol Appl Pharmacol. 280:285. PubMed
  26. Liu J, et al. 2015. J Mol Cell Cardiol. 80:10. PubMed
Product Citations
  1. Derudder E, et al. 2016. Proc Natl Acad Sci U S A. 113: 5065-5070. PubMed
  2. Zhang T, et al. 2022. EBioMedicine. 77:103872. PubMed
  3. Dhoonmoon A, et al. 2022. Nat Commun. 13:5063. PubMed
  4. Glynn RA, et al. 2022. J Immunol. 208:371. PubMed
  5. Cao H, et al. 2022. Br J Haematol. 198:155. PubMed
  6. Ugamraj HS, et al. 2022. MAbs. 14:2095949. PubMed
  7. Cao L, et al. 2022. Cell Rep. 40:111156. PubMed
  8. Choi UY, et al. 2022. Cell Rep. 40:111234. PubMed
  9. Kimmerling RJ, et al. 2022. Commun Biol. 5:1295. PubMed
  10. Chen X, et al. 2022. Front Pharmacol. 13:1028692. PubMed
  11. Shahid AM, et al. 2022. PLoS One. 17:e0278209. PubMed
  12. Önel T, et al. 2023. Naunyn Schmiedebergs Arch Pharmacol. :. PubMed
  13. Papakonstantinou A, et al. 2022. Int J Mol Sci. 24: . PubMed
  14. Li J, et al. 2023. J Biol Chem. 299:102875. PubMed
  15. Martinelli M, et al. 2023. iScience. 26:105860. PubMed
  16. Tay HG, et al. 2023. Mol Ther. 31:825. PubMed
  17. Dean BD, et al. 2023. J Immunother Cancer. 11: . PubMed
  18. Lieschke E, et al. 2022. Cell Death Differ. 29:1004. PubMed
  19. Zelenka T, et al. 2022. Nat Commun. 13:6954. PubMed
  20. Gallego-Murillo JS, et al. 2022. Biotechnol Bioeng. 119:3096. PubMed
  21. Du X, et al. 2023. Nucleic Acids Res. 51:1050. PubMed
  22. Vanoli F, et al. 2023. Oncogenesis. 12:8. PubMed
  23. Lei K, et al. 2023. iScience. 26:106001. PubMed
  24. Ohuchi K, et al. 2023. Biomed Rep. 18:28. PubMed
  25. Do-Thi VA, et al. 2023. Cancer Res Commun. 3:80. PubMed
  26. Perez GI, et al. 2023. Vaccines (Basel). 11:. PubMed
  27. Segaliny AI, et al. 2023. Commun Biol. 6:380. PubMed
  28. Potez M, et al. 2023. Front Oncol. 13:1124272. PubMed
  29. Woodley K, et al. 2023. Nat Commun. 14:2132. PubMed
  30. Quintela M, et al. 2023. Clin Epigenetics. 15:63. PubMed
  31. Quintela M, et al. 2023. Br J Cancer. . PubMed
  32. Yao Z, et al. 2018. J Virol. 92:00:00. PubMed
  33. Uriostegui–Arcos M, et al. 2020. Open Biol. 10:200050. PubMed
  34. Kim EH, et al. 2020. Elife. 9:00. PubMed
  35. Kitayama J, et al. 2016. PLoS One. 11: 0154542. PubMed
  36. Gedye C, et al. 2016. Sci Rep. 6: 25220. PubMed
  37. Carlsson J, et al. 2015. PLoS One. 10: 0143741. PubMed
  38. Dowling CM, et al. 2021. Sci Adv. 7:. PubMed
  39. Lin YN, et al. 2022. Oncoimmunology. 11:2027136. PubMed
  40. Bouhaddou M, et al. 2018. PLoS Comput Biol. 14:e1005985. PubMed
  41. Espinosa-Cueto P, et al. 2017. PLoS One.. 10.1371/journal.pone.0182126. PubMed
  42. Gupta A, et al. 2011. Leuk Res. 35:1498. PubMed
  43. Fujii T, et al. 2015. Proc Natl Acad Sci U S A. 112: 12800 - 12805. PubMed
  44. Cardoso B, et al. 2015. PLoS One. 10: 0143897. PubMed
  45. Alcon C, et al. 2020. Cell Death Dis. 0.898611111. PubMed
  46. Figueiredo T, et al. 2020. Mol Psychiatry. . PubMed
  47. Lopes-Coelho F, et al. 2021. Front Oncol. 11:656229. PubMed
  48. Wang Q, et al. 2022. Nat Commun. 13:3022. PubMed
  49. Olelewe C, et al. 2022. iScience. 25:104340. PubMed
  50. Yamauchi T et al. 2018. Cancer cell. 33(3):386-400 . PubMed
  51. Feng W, et al. 2017. Nat Commun. 0.697916667. PubMed
  52. Jones PD, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1711. PubMed
  53. Ramalingam P, et al. 2020. J Exp Med. 217:00:00. PubMed
  54. Kim Y, et al. 2020. J Neurochem. 155:177. PubMed
  55. Hassan MIA, et al. 2021. Comput Struct Biotechnol J. 19:880. PubMed
  56. Bowen ME, et al. 2019. Dev Cell. 50:212. PubMed
  57. Sales–Dias J, et al. 2019. PLoS One. 14:e0217002. PubMed
  58. Santos LC, et al. 2019. Nat Commun. 1.328472222. PubMed
  59. Lim JA, et al. 2018. BMC Cancer. 0.860416667. PubMed
  60. Venter C, et al. 2017. Cell Physiol Biochem. 43:1288. PubMed
  61. Soprano M, et al. 2016. PLoS One. 11: 0155970. PubMed
  62. Macpherson L, et al. 2020. Nature. 577:266. PubMed
  63. Vereertbrugghen A, et al. 2021. Front Oncol. 11:598319. PubMed
  64. Sun V, et al. 2021. Front Immunol. 12:716661. PubMed
  65. Coray M, et al. 2022. Int J Mol Sci. 23:. PubMed
  66. Karavasili C, et al. 2017. International Journal of Pharmaceutics. 10.1016/j.ijpharm.2017.05.061. PubMed
  67. Thompson PJ et al. 2019. Cell metabolism. 29(5):1045-1060 . PubMed
  68. Noguchi F, et al. 2017. Mol Cancer Ther. 1.316666667. PubMed
  69. Kumar S, et al. 2018. Cancers (Basel). 0.724305556. PubMed
  70. Fortin J, et al. 2020. Cancer Cell. 37:308. PubMed
  71. He C, et al. 2021. Oncoimmunology. 1897295:10. PubMed
  72. Pfalzgraff A, et al. 2016. Sci Rep. 6:31577. PubMed
  73. O'Neill K, et al. 2016. Genes Dev. 30: 973-988. PubMed
  74. Liu J, et al. 2015. J Mol Cell Cardiol. 80:10. PubMed
  75. Vries M, et al. 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
  76. Kim OV, et al. 2022. iScience. 25:104654. PubMed
  77. Shahzadi SK, et al. 2022. Clin Sci (Lond). 136:181. PubMed
  78. Kolbrink B, et al. 2022. Biochem J. 479:259. PubMed
  79. Chen MS, et al. 2019. Mol Cancer Res. 17:697. PubMed
  80. Ni F, et al. 2019. Cell Stem Cell. 24:608. PubMed
  81. Ning X, et al. 2019. Mol Cell. 74:19. PubMed
  82. Greenblatt SM, et al. 2018. Cancer Cell. 33:1111. PubMed
  83. Naqvi N,et al. 2017. Sci Rep.. 10.1038/s41598-017-12753-1. PubMed
  84. Wang M, et al. 2017. Biochemical and Biophysical Research Communications. 10.1016/j.bbrc.2017.05.097. PubMed
  85. Beeravolu N, McKee C, Chaudhry G 2017. J App Toxicol. 10.1002/jat.3469. PubMed
  86. Parrula M, et al. 2014. Virus Res. 189:206. PubMed
  87. Brigger D, et al. 2015. Cell Death Dis. 6: e1861. PubMed
  88. Zhang J, et al. 2016. Cell Death Dis. 7:e2266. PubMed
  89. Wang D, et al. 2016. J Cell Sci. 129: 2261 - 2272. PubMed
  90. Uchtenhagen H, et al. 2016. Nat Commun. 7:12614. PubMed
  91. Geiger R, et al. 2016. Cell. 167:829-842. PubMed
  92. Kinsella S, et al. 2021. Cell Rep. 37:109789. PubMed
  93. O'Connor MJ, et al. 2021. Oncogene. 40:4872. PubMed
  94. Esteghamat F, et al. 2019. Nat Genet. 51:1233. PubMed
  95. Blümel E, et al. 2020. Oncoimmunology. 9:1751561. PubMed
  96. Sekine C, et al. 2009. Int Immunol. 1.079861111. PubMed
  97. Madala HR, et al. 2020. Cancer Res. 3492:80. PubMed
  98. Clements KE, et al. 2020. Nat Commun. 4.706944444. PubMed
  99. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  100. Martín Monreal MT, et al. 2021. Front Immunol. 12:716250. PubMed
  101. Schmitz RL, et al. 2021. Cells. 10:. PubMed
  102. Babaei A, et al. 2021. Adv Pharm Bull. 11:361. PubMed
  103. Rauzan M, et al. 2017. PLoS One. 12:e0174107. PubMed
  104. Liu Y, et al. 2017. Acta Biomater. 10.1016/j.actbio.2017.08.021. PubMed
  105. Chiani M, et al. 2017. Artif Cells Nanomed Biotechnol. 10.1080/21691401.2017.1337029. PubMed
  106. Salge-Bartels U, et al. 2014. Thromb Res. 133:244. PubMed
  107. Passaro C, et al. 2014. Mol Oncol. 9:78. PubMed
  108. Ligtenberg M, et al. 2016. J Immunol. 196: 759 - 766. PubMed
  109. Hrdinka M, et al. 2016. PLoS One. 11: 0162863. PubMed
  110. Herold N, et al. 2017. Nat Med. 23:256-263. PubMed
  111. Xie S, et al. 2021. Sci Rep. 0.660416667. PubMed
  112. Müller I, et al. 2020. Mol Cell. 970:77. PubMed
  113. Morimoto A, et al. 2021. Nat Commun. 12:2136. PubMed
  114. Norkin M, et al. 2021. Cell Rep. 35:109026. PubMed
  115. Krauss PL, et al. 2021. Front Immunol. 12:730672. PubMed
  116. Zhu Y, et al. 2022. Clin Transl Med. 12:e887. PubMed
  117. Khatib JB, et al. 2022. Oncotarget. 13:1078. PubMed
  118. Kamiyama N, et al. 2017. Antiviral Res. 10.1016/j.antiviral.2017.08.007. PubMed
  119. Montero–Herradón S, et al. 2017. Organogenesis. 0.675. PubMed
  120. Nunes SC, et al. 2018. Sci Rep. 6.939583333. PubMed
  121. Sharif T, et al. 2018. Autophagy. 1.101388889. PubMed
  122. Eichelmann AK, et al. 2018. Sci Rep. 8:17553. PubMed
  123. Blümel E, et al. 2019. Oncoimmunology. 8:e1641387. PubMed
  124. Yoon Y, et al. 2016. Stem Cell Reports. 7:840-853. PubMed
  125. Chheda Z, et al. 2016. J Immunol. 197: 2016 - 2026. PubMed
  126. Slone E, et al. 2015. J Leukoc Biol. 98: 791 - 804. PubMed
  127. Séguier S, et al. 2013. PLoS One. 8:70937. PubMed
  128. Matsuda K, et al. 2021. Cell Rep Methods. 1:100122. PubMed
  129. Vanoli F, et al. 2021. Oncogene. 40:5095. PubMed
  130. Liu H, et al. 2021. J Immunother Cancer. 9:. PubMed
  131. Smith CM, et al. 2021. Biochem J. 478:3331. PubMed
  132. Li Y, et al. 2021. Sci Transl Med. 13:. PubMed
  133. Meyer N, et al. 2021. J Clin Med. 10:. PubMed
  134. Liu H, et al. 2020. Cell Death Dis. 0.767361111. PubMed
  135. Zervou S, et al. 2017. PLoS One.. 10.1371/journal.pone.0182994. PubMed
  136. Ishidome T et al. 2017. EBioMedicine. 22:89-99 . PubMed
  137. Wang Q, et al. 2013. Genes Dev. 27:615. PubMed
  138. Douguet L, et al. 2016. PLoS One. 11:e0166164. PubMed
  139. Toutounchian J, et al. 2016. Mol Pharmacol. 91(1):1-13. PubMed
  140. Pajarillo E, et al. 2020. J Biol Chem. 295:3040. PubMed
  141. Gholamrezaei M, et al. 2021. Iran J Parasitol. 15:475. PubMed
  142. Meyer N, et al. 2021. Int J Mol Sci. 22:. PubMed
  143. Kolbrink B, et al. 2022. Cell Mol Life Sci. 79:387. PubMed
  144. Litzenburger UM, et al. 2017. Genome Biol. 18:15. PubMed
  145. Rajavel T, et al. 2017. Sci Rep. 2.665277778. PubMed
  146. Grujic M, et al. 2010. J Immunol. 185:1730. PubMed
  147. Alsina-Sanchis E, et al. 2020. Mol Cancer Res. . PubMed
  148. L Chang, Y Li, D Kaplan 2016. Sci Rep. 6:36862. PubMed
  149. Becker A, et al. 2016. Sci Rep. 6: 33847. PubMed
  150. Kumai T, et al. 2015. Sci Rep. 5:16280. PubMed
  151. Polak R, et al. 2015. Blood. 126: 2404 - 2414. PubMed
  152. Ishida M, et al. 2021. Int J Mol Sci. 22:. PubMed
  153. Lee DH, et al. 2020. Circ Res. 127:1182. PubMed
  154. Matsumoto A, et al. 2019. J Extracell Vesicles. 9:1696517. PubMed
  155. Pirali T, et al. 2017. ChemMedChem. 1.570833333. PubMed
  156. Galski H, et al. 2013. Biochem Pharmacol. 86:584. PubMed
  157. Tsou W, et al. 2014. J Biol Chem. 289:25750. PubMed
  158. Dhoonmoon A, et al. 2020. Nucleic Acids Res. 48:7252. PubMed
  159. Nastasi C, et al. 2021. Sci Rep. 11:1458. PubMed
  160. Molinar-Inglis O, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  161. Erdogan M, et al. 2022. Chem Biodivers. 19:e202200659. PubMed
  162. Hafez A, et al. 2017. Oncogenesis. 10.1038/oncsis.2017.46. PubMed
  163. Putheti P, et al. 2010. PLoS One. 5:e8706. PubMed
  164. Cai Z, et al. 2020. Cell Rep. 31:107816. PubMed
  165. Liu B, et al. 2016. J Biol Chem. 291: 23869 - 23881. PubMed
  166. Lhuillier C, et al. 2015. J Biol Chem. 290: 16797 - 16811. PubMed
  167. Passaro C, et al. 2013. Endocr Relat Cancer. 20:633. PubMed
  168. Mulik R, et al. 2010. Int J Pharm. 398:190. PubMed
  169. Speth C, et al. 2013. J Infect Dis. 207:823. PubMed
  170. Shadboorestan A, et al. 2022. Iran J Basic Med Sci. 25:506. PubMed
  171. Huang Y, et al. 2022. Front Cell Dev Biol. 9:837777. PubMed
  172. Babaei A, et al. 2020. Daru. 28:555. PubMed
  173. King R, et al. 2021. Sci Adv. 7:eabj5293. PubMed
  174. Gladow N, et al. 2020. PLoS One. 15:e0227734. PubMed
  175. Lee HW, et al. 2018. Am J Physiol Renal Physiol. 314:F280. PubMed
  176. Ki H, et al. 2017. Biomed Pharmacother. 10.1016/j.biopha.2017.09.118. PubMed
  177. Lin W, et al. 2014. PLoS One. 9:83160. PubMed
  178. Zhang X, et al. 2014. Acta Biochim Biophys Sin. 46:261. PubMed
  179. CO L, et al. 2015. Nat Commun. 6: 7739. PubMed
  180. Huang YL, et al. 2020. Elife. 9:00. PubMed
  181. Abu El Maaty MA, et al. 2021. Sci Adv. 7: . PubMed
  182. Bahl S, et al. 2021. Cell Death Dis. 12:469. PubMed
  183. Jackson LM, et al. 2021. Nucleic Acids Res. 49:12855. PubMed
  184. Schuler F, et al. 2017. Nat Commun. . 10.1038/s41467-017-01850-4. PubMed
  185. Di Somma S, et al. 2019. Cancers (Basel). 1.70625. PubMed
  186. Faria M, et al. 2017. PLoS One. 12(2):e0172689. PubMed
  187. Jones P, et al. 2016. J Biol Chem. 291: 16318 - 16327. PubMed
  188. Awojoodu A, et al. 2014. Blood. 124:1941. PubMed
  189. Intemann J, et al. 2014. Cell Signal. 26:1567. PubMed
  190. Tatangelo V, et al. 2022. Front Oncol. 12:877495. PubMed
  191. Alcon C, et al. 2021. Cells. 10:. PubMed
  192. Cao H, et al. 2021. Nat Commun. 12:1792. PubMed
  193. Labi V, et al. 2019. Genes Dev. 33:1673. PubMed
  194. Fang P, et al. 2019. Commun Biol. 0.315972222. PubMed
  195. Kumar S, et al. 2019. Int J Cancer. 146:1652. PubMed
  196. Zhao CC, et al. 2018. Oncol Rep. 40:3392. PubMed
  197. Schleicher EM, et al. 2018. Nucleic Acids Res. 46:8908. PubMed
  198. Thomas A, et al. 2017. PLoS One.. 10.1371/journal.pone.0183542. PubMed
  199. Bostanabad SY, et al. 2021. Sci Rep. 11:1539. PubMed
  200. Monzen S, et al. 2022. Mol Clin Oncol. 16:19. PubMed
  201. Konda P, et al. 2022. Am J Cancer Res. 12:210. PubMed
  202. Wu J, et al. 2021. Adv Sci (Weinh). 8:e2101029. PubMed
  203. Clements KE, et al. 2018. Nucleic Acids Res. 46:8898. PubMed
  204. Zhang Y, et al. 2021. Cell Death Dis. 0.563194444. PubMed
  205. Kang JH, et al. 2020. Cell Death Dis. 0.803472222. PubMed
  206. Dewdney B, et al. 2020. Sci Rep. 10:16769. PubMed
  207. Simoni L, et al. 2020. Cell Rep. 33:108330. PubMed
  208. Coutaz M, et al. 2016. Sci Rep. 6:39117. PubMed
  209. Akane K, et al. 2016. Proc Natl Acad Sci U S A. 113: 2460 - 2465. PubMed
  210. Xiong R, et al. 2014. Toxicol Appl Pharmacol. 280:285. PubMed
  211. Chen Y, et al. 2013. J Exp Med. 210:2515. PubMed
  212. Tu Z, et al. 2022. Cell Rep. 40:111072. PubMed
  213. Bensberg M, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  214. Paparella AS, et al. 2021. Nat Commun. 12:6285. PubMed
  215. Huang YL, et al. 2021. iScience. 24:103168. PubMed
  216. Mendès C, et al. 2019. Sci Rep. 9:14107. PubMed
  217. Lopes–Coelho F, et al. 2017. Oncotarget. 8:82803. PubMed
  218. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  219. Thanuthanakhun N, et al. 2017. PLoS One. . 10.1371/journal.pone.0187610. PubMed
  220. Kuntz E, et al. 2017. Nat Med. 10.1038/nm.4399. PubMed
  221. Bottardi S, et al. 2020. Exp Hematol. 68:88. PubMed
  222. Kasikara C, et al. 2021. J Clin Invest. 131:. PubMed
  223. Ning N, et al. 2022. Theranostics. 12:5537. PubMed
  224. Siddhartha V, et al. 2017. Artif Cells Nanomed Biotechnol . 10.1080/21691401.2017.1313267. PubMed
  225. He J, et al. 2017. FASEB J. 31:2893. PubMed
  226. Lyu X, et al. 2019. BMC Genomics. 1.235416667. PubMed
  227. Jing Y, et al. 2019. J Allergy Clin Immunol. 144:1377. PubMed
  228. Quan M, et al. 2019. Oncol Rep. 41:3051. PubMed
  229. Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed
  230. Pahari S, et al. 2020. Autophagy. 1.375694444. PubMed
  231. Schleicher EM, et al. 2020. PLoS Genet. 16:e1009176. PubMed
  232. Langsch S, et al. 2016. Cancer Res . 76: 4160 - 4169. PubMed
  233. Xiong R, et al. 2015. Mol Pharmacol. 88: 1045 - 1054. PubMed
  234. Hellmann J, et al. 2013. J Immunol. 191:1383. PubMed
  235. Juel H, et al. 2013. PLoS One. 8:64619. PubMed
  236. Gill K, et al. 2013. Biochim Biophys Acta. 1830:2763. PubMed
  237. Wang Y, et al. 2022. Front Cardiovasc Med. 9:876087. PubMed
  238. Shankman LS, et al. 2021. Curr Biol. 31:2469. PubMed
  239. Young MM, et al. 2019. Cell Death Dis. 1.004861111. PubMed
  240. Santos I, et al. 2019. Nutrients. 2.210416667. PubMed
  241. Vancsik T, et al. 2019. Cancer Med. 3.313888889. PubMed
  242. Zheng M, et al. 2019. Proc Natl Acad Sci U S A. 116:12422. PubMed
  243. Zheng Q, et al. 2017. Mol Oncol. 10.1002/1878-0261.12056. PubMed
RRID
AB_2561291 (BioLegend Cat. No. 640905)
AB_2561292 (BioLegend Cat. No. 640906)
AB_2629519 (BioLegend Cat. No. 640945)

Antigen Details

Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience
Gene ID
308 View all products for this Gene ID
UniProt
View information about Annexin V on UniProt.org

Related FAQs

How is your Annexin made and what sequence does it cover?

It is made in E. coli, covering human aa Met1-Asp320.

How does pH and staining temperature affect Annexin V-Phosphatidylserine binding?

Annexin-Phosphatidylserine binding is lost below pH 5.2 and with prolonged incubation over a temperature of 42°C.

Why do I need to use Annexin V Binding Buffer?

Annexin V binding requires the presence of calcium in the solution.  So, we provide Annexin V Binding Buffer (cat # 422201), which is optimized for the best performance of Annexin V staining.

Can I use RPMI during Annexin V staining?

It is best to follow protocol as described on the product data sheet. Moreover, RPMI 1640 has a relatively high concentration of phosphate and low calcium ion concentration, which negatively impacts Annexin binding to its target phosphatidylserine (PS). Measurement of cell death by using Annexin V may also be significantly affected by time of incubation on ice, calcium concentration, and type of medium.

Can I freeze Annexin V conjugates?

It should not be frozen as it will lead to loss of biological activity due to dimerization.

Is Annexin V suitable for conjugation with the Maxpar® kit for CyTOF®?

Maxpar® Labeling kits require the protein to be partially reduced, so the metal chelate can be introduced through an SH group in the hinge region of the reduced antibody. Human Annexin V contains only one Cysteine which was reported to be chemically inactive. Thus, the Maxpar® labeling protocol would not work with Annexin V, unless a free –SH group can be introduced to Annexin V.  For more information regarding SH-mediated conjugation of Annexin V please consult published papers such as this one.

Go To Top Version: 7    Revision Date: 10/22/2024

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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