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FITC Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
Streptavidin-Fluorescein Isothiocyanate, SAv-FITC
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Product Citations
publications
SAV_FITC_082808.jpg
C57BL/6 splenocytes stained with 145-2C11 biotin, followed by Sav-FITC
  • SAV_FITC_082808.jpg
    C57BL/6 splenocytes stained with 145-2C11 biotin, followed by Sav-FITC
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405201 100 µg $42
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405202 500 µg $118
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Description

Streptavidin binds to biotin with high affinity. Streptavidin-fluorescein isothiocyanate (FITC) is useful for detecting biotinylated antibodies. The excitation of FITC by 488 nm laser light induces a light emission maximum of 520 nm.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin is conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The Streptavidin-FITC solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

ICFC - Verified
Recommended Usage

Each lot of this Streptavidin-FITC is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the FITC conjugation. For immunofluorescent staining, the recommended use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Streptavidin-FITC is useful as a second step reagent for indirect immunofluorescent staining, in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-FITC is 60 kD and Streptavidin alone is 52 kD.

Application References

(PubMed link indicates BioLegend citation)
  1. Schmutz S, et al. 2009. J. Immunol. 183:2217. PubMed
  2. Cho HY, et al. 2009. Immunol Lett. 127:39. PubMed
  3. de Wispelaere D, et al. 2012. J. Virol. 86:7072. PubMed
  4. Fukuoka A, et al. 2013. Int Immunol. PubMed
  5. Giltiay NV, et al. 2013. J Exp Med. 25:373. PubMed
  6. Lee JH, et al. 2014. J Rheumatol. 41:2425. PubMed
Product Citations
  1. Kemble S, et al. 2022. Blood Adv. 6:2932. PubMed
  2. Glass MC, et al. 2022. Cell Rep. 39:110728. PubMed
  3. Lorenzo-Martín LF, et al. 2022. Oncogene. 41:3341. PubMed
  4. Lin G, et al. 2022. Int J Immunopathol Pharmacol. 36:3946320221117933. PubMed
  5. Parnigoni A, et al. 2022. Matrix Biol. 109:140. PubMed
  6. Du J, et al. 2022. Sci Immunol. 7:eabo5407. PubMed
  7. Pymm P, et al. 2022. iScience. 25:105259. PubMed
  8. Yu H, et al. 2023. Nat Commun. 14:1058. PubMed
  9. Luo Y, et al. 2023. Cell Discov. 9:40. PubMed
  10. Chen K, et al. 2023. Gut Pathog. 15:28. PubMed
  11. Georgiadis C, et al. 2021. Leukemia. . PubMed
  12. Larsen SB, et al. 2021. Cell Stem Cell. 28:1758. PubMed
  13. Suarez-Calvet X, et al. 2021. FASEB J. 35:e21819. PubMed
  14. Zaitouna AJ, et al. 2020. Bio Protoc. 10:e3655. PubMed
  15. Krishack PA, et al. 2019. JCI Insight. 4:6. PubMed
  16. Sasaki N, Itakura Y, Toyoda M 2017. Stem Cell Res Ther. 10.1186/s13287-017-0534-1. PubMed
  17. Yang M 2012. J Virol. 86:7072. PubMed
  18. Pan H, et al. 2020. Mol Psychiatry. . PubMed
  19. Wang Y, et al. 2021. Nat Commun. 12:2343. PubMed
  20. Sánchez-Salguero E, et al. 2021. Front Immunol. 12:712130. PubMed
  21. Hu Y, et al. 2022. Nat Commun. 13:5313. PubMed
  22. Li S, et al. 2016. PLoS One. 11: 0163893. PubMed
  23. Lee J, et al. 2014. J Rheumatol. 41:2425. PubMed
  24. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  25. Vigeland C, et al. 2016. PLoS One. 11: 0163288. PubMed
  26. Morimoto A, et al. 2021. Nat Commun. 12:2136. PubMed
  27. Noviski M, et al. 2018. Elife. 7:e35074. PubMed
  28. Zhang Y, et al. 2019. JCI Insight. 4:2. PubMed
  29. Yan R, et al. 2021. Cell Death Dis. 12:955. PubMed
  30. Cheng LS, et al. 2022. J Transl Med. 20:415. PubMed
  31. Shafiei-Jahani P, et al. 2021. Nat Commun. 12:2526. PubMed
  32. Galle-Treger L, et al. 2016. Nat Commun. 7:13202. PubMed
  33. Schmutz S, et al. 2009. J Immunol. 183:2217. PubMed
  34. Delacher M, et al. 2020. Immunity. 52:295. PubMed
  35. Galle-Treger L, et al. 2019. Nat Commun. 10:713. PubMed
  36. Matt P, et al. 2015. PLoS One. 10: 0137474. PubMed
  37. Bülow JM, et al. 2022. Front Endocrinol (Lausanne). 13:1016057. PubMed
  38. Oda Y, et al. 2021. Sci Adv. 7:eabj6895. PubMed
  39. Li X, et al. 2021. Oncol Lett. 22:788. PubMed
  40. Buffolo M, et al. 2019. Cell Rep. 29:270. PubMed
  41. Cho H, et al. 2009. Immunol Lett. 127:39. PubMed
  42. Wang Y, et al. 2022. Front Immunol. 13:847581. PubMed
  43. Hensel JA, et al. 2019. Mol Ther Oncolytics. 12:41. PubMed
  44. Mödinger Y, et al. 2018. J Cell Mol Med. 22:6002. PubMed
  45. Lin Y, et al. 2012. PLoS One. 7:e30507. PubMed
  46. Fukuoka A, et al. 2013. Int Immunol. 25:373. PubMed
  47. Giltiay N, et al. 2013. J Exp Med. 210:2773. PubMed
  48. Mchedlidze T, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.20. PubMed
  49. SL C, et al. 2016. JCI Insight. 1: 83116. PubMed
  50. Hamester F, et al. 2022. Cells. 11:. PubMed
  51. Laurin M, et al. 2019. Elife. 8. PubMed
  52. Sturmlechner I, et al. 2021. Science. 374:eabb3420. PubMed
  53. Lindsay RS, et al. 2021. J Exp Med. 218:. PubMed
  54. Vogiatzi A, et al. 2021. Mol Cell Biol. 41:e0014921. PubMed
  55. Geng S, et al. 2016. Nat Commun. 7:13436. PubMed
  56. Zhu Z, et al. 2017. Int J Biochem Cell Biol. 10.1016/j.biocel.2017.04.003. PubMed
Go To Top Version: 5    Revision Date: 06/17/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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