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MojoSort™ Mouse CD4 T Cell Isolation Kit

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MCD4TCellIsoKit_1_051215
A single cell suspension from pooled C57BL/6 mouse lymphoid tissues was prepared to isolate CD4+ T cells using the MojoSort™ Mouse CD4 T Cell Isolation Kit. Cells were stained with CD4 (clone RM4-4) PE and CD3ε (145-2C11) APC.
  • MCD4TCellIsoKit_1_051215
    A single cell suspension from pooled C57BL/6 mouse lymphoid tissues was prepared to isolate CD4+ T cells using the MojoSort™ Mouse CD4 T Cell Isolation Kit. Cells were stained with CD4 (clone RM4-4) PE and CD3ε (145-2C11) APC.
  • MCD4TCellIsoKit_2_051215
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480005 10 tests $113
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480006 100 tests $483
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480033 200 tests $654
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Description

Non CD4+ T cells are depleted by incubating your sample with the biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched CD4+ T cells are collected by decanting the liquid in a clean tube. These are your cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc.

MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.

Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.

Product Details
Technical data sheet

Kit Contents

Kit Contents

For Cat# 480005:

  • 100 µl Biotin-Antibody Cocktail
  • 100 µl Streptavidin Nanobeads

For Cat# 480006:

  • 1 ml Biotin-Antibody Cocktail
  • 1 ml Streptavidin Nanobeads

For Cat# 480033:

  • 2 vials of 1 ml Biotin-Antibody Cocktail each
  • 2 vials of 1 ml Streptavidin Nanobeads each

Product Details

Verified Reactivity
Mouse
Formulation
Cocktail: Phosphate-buffered solution containing 0.09% sodium azide, pH 7.2.
Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide.
Preparation
The antibodies were purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Streptavidin Nanobeads: Streptavidin-coated magnetic beads.
Storage & Handling
Antibody cocktail and Streptavidin Nanobeads should be stored undiluted between 2°C and 8°C.
Application

Cell Separation (MojoSort™) - Quality tested
Recommended Usage

10 µl of antibody cocktail for 1 X 107 cells in 100 µl of buffer.
10 µl Streptavidin Nanobeads for 1 X 107 cells in 100 µl of buffer.

Application Notes

This kit is designed for the isolation of untouched CD4+ T cells from lymphoid tissues.

Each lot has been individually optimized. Do not mix and match components from different lots or different kits.
 

Antibody or cocktail dilution to use in column: 8X
Nanobead dilution to use in columns: 9X

Application References

(PubMed link indicates BioLegend citation)
  1. Dong L, et al. 2016. Sci. Rep. 6:36598. (PubMed)
Product Citations
  1. Fernandez-Perez R, et al. 2021. Front Immunol. 12:687443. PubMed
  2. Tatsumi N, et al. 2022. STAR Protoc. 3:101845. PubMed
  3. Guo H, et al. 2022. Biomaterials. 283:121441. PubMed
  4. Hochrein SM, et al. 2022. Cell Metab. 34:516. PubMed
  5. Du J, et al. 2022. Sci Immunol. 7:eabo5407. PubMed
  6. Li L, et al. 2022. Cancer Immunol Res. 10:1475. PubMed
  7. Hao S, et al. 2023. EMBO Rep. 24:e56932. PubMed
  8. Liu T, et al. 2023. J Neuroinflammation. 20:94. PubMed
  9. Park JS, et al. 2023. Nat Commun. 14:2980. PubMed
  10. Pandey S, et al. 2020. Cell Syst. 11:495. PubMed
  11. Pei S, et al. 2021. J Exp Med. 218:. PubMed
  12. Lin CH, et al. 2021. J Exp Med. 218:. PubMed
  13. Ecker M, et al. 2022. Elife. 11:. PubMed
  14. Guzman–Genuino RM, et al. 2020. Front Immunol. 2.429861111. PubMed
  15. Dong L, et al. 2017. Sci Rep. 10.1038/srep36598. PubMed
  16. Elyada E, et al. 2019. Cancer Discov. 1.140277778. PubMed
  17. Tatsumi N, et al. 2021. Sci Immunol. 6:eabg0336. PubMed
  18. Pan YG, et al. 2017. Front Immunol. 1.322222222. PubMed
  19. Tang X, et al. 2022. Cell Rep. 41:111673. PubMed
  20. Cheng Y, et al. 2020. PLoS Pathog. 16:e1008569. PubMed
  21. Bai C, et al. 2020. Mol Ther Oncolytics. 17:9. PubMed
  22. Kanno T, et al. 2022. Front Immunol. 13:904875. PubMed
  23. Bocchini C, et al. 2016. Blood. 128(26):3061-3072. PubMed
  24. Woodring T, et al. 2022. iScience. 25:104934. PubMed
  25. Feng P, et al. 2021. Theranostics. 11:9503. PubMed
  26. Kumar V, et al. 2022. JACC Basic Transl Sci. 7:1038. PubMed
  27. Yin X, et al. 2020. Cell Rep. 33:108278. PubMed
  28. Jain A, et al. 2020. Nat Immunol. 0.920138889. PubMed
  29. Nomura S, et al. 2019. Sci Rep. 9:17335. PubMed
  30. Qin J, et al. 2020. Cancer Immunol Res. 8:1426. PubMed
  31. Wang N, et al. 2020. Front Immunol. 1.765972222. PubMed

Antigen Details

Biology Area
Immunology
Molecular Family
CD Molecules
Gene ID
NA
UniProt
View information about CD4 on UniProt.org

Related FAQs

Is there a way to detach your magnetic particles from the cell surface?

MojoSort™ Nanobeads: These magnetic particles cannot be removed from cells. This includes standalone Nanobeads and MojoSort kits using Nanobeads. We have found that cells are functional without the need to detach the magnetic Nanobeads.

 

MojoSort Microbeads: These magnetic particles may be released following the detailed product-specific protocol.

What is the size of your magnetic particles?

MojoSort Nanobeads: the average diameter is approximately 130 nm.

 

MojoSort Microbeads: the average diameter is approximately 1 μm.

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more product-specific information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

What antibodies are present in the depletion cocktails provided for isolation kits?

Please contact our technical service team for further assistance.

Go To Top Version: 4    Revision Date: 01/26/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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