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Pacific Blue™ anti-human CD19 Antibody

Pricing & Availability
Clone
HIB19 (See other available formats)
Regulatory Status
RUO
Workshop
V CD19.11
Other Names
B4
Isotype
Mouse IgG1, κ
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Product Citations
publications
HIB19_Blue_022008
Human peripheral blood lymphocytes stained with HIB19 Pacific Blue™
  • HIB19_Blue_022008
    Human peripheral blood lymphocytes stained with HIB19 Pacific Blue™
Compare all formats See Pacific Blue™ spectral data
Cat # Size Price Quantity Check Availability Save
302223 25 µg $124
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302232 100 tests $264
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302224 100 µg $265
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Description

CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B-cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
µg sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
µg sizes: 0.5 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The CD19 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.
For test sizes, the suggested use of this reagent for immunofluorescent staining is 5 µl per 106 cells in 100 µl volume.
For µg sizes, the suggested use of this reagent for immunofluorescent staining is ≤ 0.5 µg per 106 cells in 100 µl volume.
It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections8 and blocking of B cell proliferation. Clone HIB19 is not recommended for formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302267 & 302268).

 

Clone HIB19 partially blocks anti-human CD19 clones 4G7 and SJ25C1 staining based on in-house testing

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. Bradbury L, et al. 1993. J. Immunol. 151:2915.
  4. Joseph A, et al. 2010. J. Virol. 84:6645. PubMed
  5. Wang X, et al. 2010. Haematologica. 95:884. (FC) PubMed
  6. Walker JD, et al. 2009. J. Immunol. 182:1548. (Block) PubMed
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  8. Hansen A, et al. 2002. Arthritis Rheum. 46:2160. (IHC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  10. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Ghassemi S, et al. 2022. Nat Biomed Eng. 6:118. PubMed
  2. Fu J et al. 2019. Cell stem cell. 24(2):227-239 . PubMed
  3. Li M, et al. 2023. Front Immunol. 14:1087923. PubMed
  4. Macal M, et al. 2016. J Immunol. 196: 1900 - 1909. PubMed
  5. Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed
  6. Savage TM, et al. 2018. JCI Insight. 3:. PubMed
  7. Boer M, et al. 2015. Clin Vaccine Immunol. 22: 778 - 788. PubMed
  8. Greenshields-Watson A, et al. 2020. Cell Rep. 32:107885. PubMed
  9. Dockree T, et al. 2016. Immunol Cell Biol. 95:68-76. PubMed
  10. Chen YQ et al. 2018. Cell. 173(2):417-429 . PubMed
  11. Dolton G, et al. 2022. Cell. 185:2936. PubMed
  12. Ogishi M, et al. 2021. Nat Med. 27:1646. PubMed
  13. Koning D, et al. 2013. J Immunol. 190:391. PubMed
  14. Gauvin J, et al. 2016. PLoS One. 11: 0155868. PubMed
  15. Yang Z, et al. 2018. Front Immunol. 9:2613. PubMed
  16. Queckborner S, et al. 2020. Stem Cell Res Ther. 11:15. PubMed
  17. Vetsika EK, et al. 2021. Cancers (Basel). 13:. PubMed
  18. Chen L, et al. 2016. Ann Rheum Dis. 75: 916 - 923. PubMed
  19. Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed
  20. Santos J, et al. 2013. J Transl Med. 11:18. PubMed
  21. Song M, et al. 2022. J Gene Med. 24:e3455. PubMed
  22. Osorio FG et al. 2018. Cell reports. 25(9):2308-2316 . PubMed
  23. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  24. Della-Torre E, et al. 2015. Ann Rheum Dis. 74: 2236 - 2243. PubMed
  25. Zuccato C, et al. 2022. Ther Adv Hematol. 13:20406207221100648. PubMed
  26. Tungatt K, et al. 2015. J Immunol. 194:463. PubMed
  27. Ying Zhang et al. 2017. Cancer cell. 32(3):377-391 . PubMed
  28. Autenrieth S, et al. 2015. Clin Transl Immunology. 4:50. PubMed
  29. Lee-Chang C, et al. 2021. J Exp Med. 218:. PubMed
  30. Rouers A, et al. 2021. iScience. 24:102482. PubMed
  31. Barman S, et al. 2016. Int Immunol. 28: 533 - 545. PubMed
  32. Linley A, et al. 2015. Blood. 126: 1902 - 1910. PubMed
  33. Bartholomaeus P, et al. 2014. J Immunol. 192:2091. PubMed
  34. Dulberger CL et al. 2017. Immunity. 46(6):1018-1029 . PubMed
  35. Rius C, et al. 2018. J Immunol. 200:2263. PubMed
  36. Darcis G, et al. 2020. Cell Rep. 30:2284. PubMed
  37. Drennan S, et al. 2016. Leukemia. 10.1038/leu.2016.356. PubMed
  38. Holland C, et al. 2015. J Immunol. 195: 5827 - 5836. PubMed
  39. Steinsbø &, et al. 2014. Nat Commun. 5:4041. PubMed
  40. Lindeman I, et al. 2021. J Exp Med. 218:. PubMed
  41. Serr I, et al. 2016. Nat Commun. 7:10991. PubMed
  42. Li M, et al. 2020. Nat Commun. 4051:11. PubMed
  43. Yang C, et al. 2019. Nat Commun. 10:3931. PubMed
  44. Shao S, et al. 2020. Proc Natl Acad Sci U S A. 117:8845. PubMed
  45. Welch JL, et al. 2020. FEBS Lett. 594:695. PubMed
  46. Malarkey C, et al. 2016. PLoS One. 11: 0157157. PubMed
  47. Longino NV, et al. 2019. Cancer Immunol Res. 7:1727. PubMed
RRID
AB_2073118 (BioLegend Cat. No. 302223)
AB_2073118 (BioLegend Cat. No. 302232)
AB_2073118 (BioLegend Cat. No. 302224)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 95 kD
Distribution

B lineage (except plasma cells), follicular dendritic cells

Function
B cell activation and differentiation
Ligand/Receptor
Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Tedder T, et al. 1994. Immunol. Today 15:437.
2. Bradbury L, et al. 1993. J. Immunol. 151:2915.

Gene ID
930 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Other Formats

View All CD19 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD19 HIB19 FC
Biotin anti-human CD19 HIB19 FC
FITC anti-human CD19 HIB19 FC
PE anti-human CD19 HIB19 FC
PE/Cyanine5 anti-human CD19 HIB19 FC
Purified anti-human CD19 HIB19 FC,CyTOF®,IHC-F
APC/Cyanine7 anti-human CD19 HIB19 FC
PE/Cyanine7 anti-human CD19 HIB19 FC
Alexa Fluor® 488 anti-human CD19 HIB19 FC,ICC
Alexa Fluor® 647 anti-human CD19 HIB19 FC,IHC-F
Pacific Blue™ anti-human CD19 HIB19 FC
Alexa Fluor® 700 anti-human CD19 HIB19 FC
PerCP anti-human CD19 HIB19 FC
PerCP/Cyanine5.5 anti-human CD19 HIB19 FC
Brilliant Violet 421™ anti-human CD19 HIB19 FC,ICC,IHC-F
Brilliant Violet 570™ anti-human CD19 HIB19 FC
Brilliant Violet 650™ anti-human CD19 HIB19 FC
Brilliant Violet 785™ anti-human CD19 HIB19 FC
Brilliant Violet 510™ anti-human CD19 HIB19 FC
Brilliant Violet 605™ anti-human CD19 HIB19 FC
Brilliant Violet 711™ anti-human CD19 HIB19 FC
Purified anti-human CD19 (Maxpar® Ready) HIB19 FC,CyTOF®
Alexa Fluor® 594 anti-human CD19 HIB19 ICC,IHC-F
PE/Dazzle™ 594 anti-human CD19 HIB19 FC
PE anti-human CD19 HIB19 FC
APC/Fire™ 750 anti-human CD19 HIB19 FC
Pacific Blue™ anti-human CD19 HIB19 FC
APC anti-human CD19 HIB19 FC
PE/Cyanine7 anti-human CD19 HIB19 FC
TotalSeq™-A0050 anti-human CD19 HIB19 PG
Brilliant Violet 750™ anti-human CD19 HIB19 FC
TotalSeq™-B0050 anti-human CD19 HIB19 PG
TotalSeq™-C0050 anti-human CD19 HIB19 PG
PerCP/Cyanine5.5 anti-human CD19 HIB19 FC
Spark NIR™ 685 anti-human CD19 HIB19 FC
Ultra-LEAF™ Purified anti-human CD19 HIB19 FC,CyTOF®,Block,IHC
APC/Fire™ 810 anti-human CD19 HIB19 FC
PE/Fire™ 640 anti-human CD19 HIB19 FC
PE/Fire™ 700 anti-human CD19 HIB19 FC
TotalSeq™-D0050 anti-human CD19 HIB19 PG
Spark YG™ 593 anti-human CD19 HIB19 FC
GMP Pacific Blue™ anti-human CD19 HIB19 FC
Spark Violet™ 423 anti-human CD19 HIB19 FC
GMP PE anti-human CD19 HIB19 FC
GMP APC anti-human CD19 HIB19 FC
KIRAVIA Blue 520™ anti-human CD19 HIB19 FC
GMP PerCP/Cyanine5.5 anti-human CD19 HIB19 FC
GMP PE/Cyanine7 anti-human CD19 HIB19 FC
Spark Violet™ 500 anti-human CD19 HIB19 FC
PE/Fire™ 810 anti-human CD19 HIB19 FC
Spark Violet™ 423 anti-human CD19 HIB19 FC
Spark UV™ 387 anti-human CD19 HIB19 FC
APC/Fire™ 750 anti-human CD19 HIB19 FC
PE/Cyanine5 anti-human CD19 HIB19 FC
Alexa Fluor® 660 anti-human CD19 HIB19 FC
PerCP/Fire™ 806 anti-human CD19 HIB19 FC
PerCP/Fire™ 780 anti-human CD19 HIB19 FC
Spark PLUS UV395™ anti-human CD19 HIB19 FC
Spark Red™ 718 anti-human CD19 HIB19 FC
FITC anti-human CD19 HIB19 FC
Go To Top Version: 2    Revision Date: 12/18/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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