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PE anti-human CD125 (IL-5Rα) Antibody

Pricing & Availability
Clone
S20015E (See other available formats)
Regulatory Status
RUO
Other Names
IL5RA, IL5R, CDw125, HSIL5R3
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
S20015E_PE_CD125_Antibody_1_032923
Human peripheral blood granulocytes were stained with anti-human CD193/CCR3 Brilliant Violet 421™ and anti-human CD125 (IL-5Rα) (clone S20015E) PE (left) or mouse IgG1, κ PE isotype control (right).
  • S20015E_PE_CD125_Antibody_1_032923
    Human peripheral blood granulocytes were stained with anti-human CD193/CCR3 Brilliant Violet 421™ and anti-human CD125 (IL-5Rα) (clone S20015E) PE (left) or mouse IgG1, κ PE isotype control (right).
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384703 25 tests $165
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384704 100 tests $363
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Description

CD125, also known as IL5 receptor α-chain, is a 60 kD type I transmembrane protein that binds IL-5 and associates in a heterodimer with the signal-transducing β subunit CD131 to form the IL-5 receptor. The affinity of CD125 for IL-5 is increased when is associated with CD131. The cytoplasmic region of CD125 activates the JAK2/STAT5 pathway and the kinase Btk. CD125 is expressed by eosinophils, basophils, mast cells, B1 cells and a subset of resting B2 cells and have a central role in the differentiation, activation and survival of these cells. Due to the restricted expression of this molecule, it is considered a potential therapeutic target for allergic inflammatory conditions.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mouse IL-5Rα transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2936755 (BioLegend Cat. No. 384703)
AB_2936755 (BioLegend Cat. No. 384704)

Antigen Details

Structure
Type I transmembrane protein, 60 kD
Distribution

Eosinophils, basophils, mast cells, B1 cells, subset of resting B2 cells

Function
Role in eosinophils and basophils differentiation, activation and survival
Interaction
JAK2/STAT, Btk, SOX4
Ligand/Receptor
IL-5
Cell Type
B cells, Basophils, Eosinophils
Biology Area
Cell Biology, Cell Proliferation and Viability, Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References
  1. Buchheit KM, et al. 2020. J Allergy Clin Immunol. 145:1574-1584.
  2. Lin X, et al. 2020. Inflammation. 43:1797-1805.
  3. Gorski SA, et al. 2019. PLoS One. 14:e0221113.
  4. Varricchi G, et al. 2016. Curr Opin Allergy Clin Immunol.16:186-200.
  5. Beekman JM. et al. 2009. Blood. 114:3917-27.
  6. Lei JT. et al. 2008. J Leukoc Biol. 84:499-509.
Gene ID
3568 View all products for this Gene ID
UniProt
View information about CD125 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 03/06/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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