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PE anti-mouse AhR Antibody

Pricing & Availability
Clone
W16012C (See other available formats)
Regulatory Status
RUO
Other Names
bHLHe76, aryl hydrocarbon receptor, Aryl hydrocarbon receptor, RP85
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W16012C_PE_AhR_Antibody_042621
C57BL/6 mouse splenocytes were stimulated with (A) or without (B) recombinant human TGF-β1 and recombinant mouse IL-6 for 3 days. Cells were surface stained with anti-mouse CD4 APC, fixed and permeabilized, then intracellularly stained with anti-mouse AhR PE (clone W16012C).
  • W16012C_PE_AhR_Antibody_042621
    C57BL/6 mouse splenocytes were stimulated with (A) or without (B) recombinant human TGF-β1 and recombinant mouse IL-6 for 3 days. Cells were surface stained with anti-mouse CD4 APC, fixed and permeabilized, then intracellularly stained with anti-mouse AhR PE (clone W16012C).
See PE spectral data
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162703 25 µg $214
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Description

Aryl Hydrocarbon Receptor or AhR is a bHLH transcription factor that is activated by ligands such as TCDD. It is expressed in many immune cells and perform an important role in immune regulation. AhR is induced under TH17-polarizing conditions. In its inactive state, AhR presents in the cytoplasm, and upon ligand binding it translocates to the nucleus. Activation of AhR is tightly controlled by Ubiquitination, ligand metabolism, and complex disruption.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2892335 (BioLegend Cat. No. 162703)

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 04/26/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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