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PerCP/Cyanine5.5 anti-mouse/human CD45R/B220 Antibody

Pricing & Availability
Clone
RA3-6B2 (See other available formats)
Regulatory Status
RUO
Other Names
B220
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
RA3-6B2_PerCPCyanine55_CD45R-B220_Antibody_091919.png
C57BL/6 mouse splenocytes stained with CD3 APC and CD45R/B220 (clone RA3-6B2) PerCP/Cyanine5.5 (left) or Rat IgG2a, κ PerCP/Cyanine5.5 isotype control (right)
  • RA3-6B2_PerCPCyanine55_CD45R-B220_Antibody_091919.png
    C57BL/6 mouse splenocytes stained with CD3 APC and CD45R/B220 (clone RA3-6B2) PerCP/Cyanine5.5 (left) or Rat IgG2a, κ PerCP/Cyanine5.5 isotype control (right)
Compare all formats See PerCP/Cyanine5.5 spectral data
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103235 25 µg $90
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103236 100 µg $245
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Description

CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Human
Reported Reactivity
Cat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Abelson murine leukemia virus-induced pre-B tumor cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo modulation of B cell responses2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6, and spatial biology (IBEX)14,15.

Application References

(PubMed link indicates BioLegend citation)
  1. Coffman RL. 1982. Immunol. Rev. 69:5. (IP)
  2. George A, et al. 1994. J. Immunol. 152:1014. (Activ)
  3. Asensi V, et al. 1989. Immunology 68:204. (Activ)
  4. Domiati-Saad R, et al. 1993. J. Immunol. 151:5936. (Activ)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Monteith CE, et al. 1996. Can. J. Vet. Res. 60:193. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Chang C L-T, et al. 2007. J. Immunol. 178:6984.
  9. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  10. Lang GL, et al. 2008. Blood 111:2158. PubMed
  11. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  12. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  13. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  14. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  15. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Sasaki I, et al. 2012. Blood. 120:4733. PubMed
  2. Lang J, et al. 2020. Nat Commun. 11:1338. PubMed
  3. Chen Q, et al. 2022. Cell Rep. 39:110990. PubMed
  4. Gupta S, et al. 2022. Sci Adv. 8:eabn4886. PubMed
  5. Arimoto KI, et al. 2023. Nat Commun. 14:251. PubMed
  6. Gon Y, et al. 2023. PLoS One. 18:e0279389. PubMed
  7. Hajjar S, et al. 2022. Cell Death Differ. 29:585. PubMed
  8. Kodali S, et al. 2022. J Immunol. 208:1085. PubMed
  9. Zong P, et al. 2022. Nat Cardiovasc Res. 1:344. PubMed
  10. Zenke S, et al. 2022. Nat Commun. 13:6459. PubMed
  11. Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed
  12. Kuang C, et al. 2023. Nat Commun. 14:2093. PubMed
  13. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  14. Macal M, et al. 2016. J Immunol. 196: 1900 - 1909. PubMed
  15. Kaku H, et al. 2014. J Immunol. 193:5904. PubMed
  16. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  17. Page N, et al. 2018. Immunity. 48:937. PubMed
  18. Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed
  19. Lin YH, et al. 2021. Front Immunol. 12:626418. PubMed
  20. Kong S, et al. 2015. J Immunol. 195: 3685 - 3693. PubMed
  21. Kobayashi T, et al. 2019. Cell. 176:982. PubMed
  22. Feng J, et al. 2021. iScience. 24:103004. PubMed
  23. Pack AD, et al. 2021. Cell Reports. 36:109586. PubMed
  24. Hirano KI, et al. 2021. eLife. 0.416666666666667. PubMed
  25. Lutzmann M, et al. 2019. Cell Rep. 28:2851. PubMed
  26. Yasuoka T, et al. 2016. PLoS One. 11: 0157395. PubMed
  27. Pan H, et al. 2020. Mol Psychiatry. . PubMed
  28. Wang X, et al. 2020. Signal Transduct Target Ther. 5:35. PubMed
  29. Elsner RA, et al. 2020. Cell Reports. 29(9):2796-2809.e5.. PubMed
  30. Fachi JL, et al. 2020. J Exp Med. 217:00:00. PubMed
  31. Kong S, et al. 2014. J Immunol. 193:5515. PubMed
  32. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  33. Sugita J, et al. 2021. Nat Commun. 12:1910. PubMed
  34. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  35. Chen L, et al. 2020. J Exp Med. 217:00:00. PubMed
  36. Stivala S, et al. 2019. J Clin Invest. 130:1596. PubMed
  37. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  38. Rivadeneira DB, et al. 2020. Immunity. 51(3):548-560. PubMed
  39. Sandborn WJ, et al. 2021. Gastroenterology. 161:1853. PubMed
  40. JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed
  41. Kotfis K, et al. 2020. British Journal of Pharmacology. 49(1):66-72. PubMed
  42. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  43. Chakraborty S, et al. 2022. Sci Transl Med. . PubMed
  44. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
  45. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  46. Pereira J, et al. 2010. PLoS One. 5:e9277. PubMed
  47. Dong S, et al. 2021. Nature. 591:117. PubMed
  48. Wang JC, et al. 2022. Elife. 11:. PubMed
  49. Yang J, et al. 2020. Nature. 586:572. PubMed
  50. Leal AS, et al. 2019. Sci Rep. 5.286111111. PubMed
  51. Schoeler K, et al. 2019. FEBS J. 10.1111/febs.14934. PubMed
  52. Melissa M Remy et al. 2017. Cell host & microbe. 22(3):354-365 . PubMed
  53. Minamitani T, et al. 2015. Proc Natl Acad Sci U S A. 112: 11612-11617. PubMed
  54. Macdougall CE et al. 2018. Cell metabolism. 27(3):588-601 . PubMed
  55. Xing Y, et al. 2022. Cancer Lett. 529:100. PubMed
  56. Webb ER, et al. 2022. iScience. 25:104995. PubMed
  57. Olson WJ, et al. 2019. Cell Rep. 28:2878. PubMed
  58. Hilpert C, et al. 2019. J Immunol. 203:3068. PubMed
  59. Beloor J et al. 2018. Cell host & microbe. 23(4):549-556 . PubMed
  60. Li Y, et al. 2021. Front Cell Dev Biol. 9:646077. PubMed
  61. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  62. Giampazolias E, et al. 2021. Cell. . PubMed
  63. Echevarría-Vargas IM, et al. 2018. EMBO Mol Med. 10:e8446. PubMed
  64. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  65. Garaycoechea JI, et al. 2018. Nature. 553:171. PubMed
  66. Srinivasan A, et al. 2021. BMC Immunol. 22:46. PubMed
  67. Shibad V, et al. 2021. Front Immunol. 12:722451. PubMed
  68. Zhai X, et al. 2021. Sci Adv. 7:eabk0490. PubMed
  69. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  70. Gorman JA, et al. 2019. Front Immunol. 10:44. PubMed
  71. Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed
  72. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  73. He C, et al. 2022. Nat Commun. 13:5459. PubMed
  74. Calderon B, et al. 2015. J Exp Med. 212: 1497-1512. PubMed
  75. Wedekind MF, et al. 2021. iScience. 24(7):102759. PubMed
  76. Ko HJ, et al. 2020. Front Immunol. 1897:11. PubMed
  77. Schuler F, et al. 2017. Nat Commun. . 10.1038/s41467-017-01850-4. PubMed
  78. Bennett FC, et al. 2018. Neuron. 98:1170. PubMed
  79. Liu B, et al. 2013. Development. 140:780. PubMed
  80. van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed
  81. Vijay R, et al. 2021. J Exp Med. 218:. PubMed
  82. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  83. Neckermann P, et al. 2021. Front Immunol. 12:761214. PubMed
  84. Hackstein CP, et al. 2022. Nat Commun. 13:7472. PubMed
  85. Zhang L, et al. 2020. Cell. 442:181. PubMed
  86. Simoni L, et al. 2020. Cell Rep. 33:108330. PubMed
  87. Wan X, Thomas J, Unanue E 2016. J Exp Med. 213: 967 - 978. PubMed
  88. Baker GJ, et al. 2020. Cell Syst. 0.647222222. PubMed
  89. Kunishita Y, et al. 2020. Front Immunol. 11:98. PubMed
  90. Li K, et al. 2020. Nat Commun. 0.795138889. PubMed
  91. Vijay R, et al. 2020. Nat Immunol. 21:790. PubMed
  92. Mincham KT, et al. 2021. Front Immunol. 11:601494. PubMed
  93. Habener A, et al. 2021. J Allergy Clin Immunol. 147:2281. PubMed
  94. Nabekura T, et al. 2020. Immunity. 96:52. PubMed
  95. Thomson CA, et al. 2018. J Immunol. 201:215. PubMed
  96. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
  97. Di Liberto G et al. 2018. Cell. 175(2):458-471 . PubMed
  98. Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
  99. Stienne C, et al. 2022. Cell Rep. 38:110553. PubMed
  100. Gu Z, et al. 2021. Nat Genet. 53:672. PubMed
RRID
AB_893354 (BioLegend Cat. No. 103235)
AB_893354 (BioLegend Cat. No. 103236)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

B cells, T cell subset, NK cell subset

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID 5788 View all products for this Gene ID
UniProt
View information about CD45R on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

View All CD45R/B220 Reagents Request Custom Conjugation
Description Clone Applications
Alexa Fluor® 594 anti-mouse/human CD45R/B220 RA3-6B2 IHC-F,FC,3D IHC
APC anti-mouse/human CD45R/B220 RA3-6B2 FC
Biotin anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F
FITC anti-mouse/human CD45R/B220 RA3-6B2 FC
PE anti-mouse/human CD45R/B220 RA3-6B2 FC
PE/Cyanine5 anti-mouse/human CD45R/B220 RA3-6B2 FC
Purified anti-mouse/human CD45R/B220 RA3-6B2 FC,CyTOF®,IHC-F,IHC-P,IP,Activ
PE/Cyanine7 anti-mouse/human CD45R/B220 RA3-6B2 FC
APC/Cyanine7 anti-mouse/human CD45R/B220 RA3-6B2 FC
Alexa Fluor® 488 anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F,3D IHC
Alexa Fluor® 647 anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F,3D IHC,SB
Pacific Blue™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Alexa Fluor® 700 anti-mouse/human CD45R/B220 RA3-6B2 FC
PerCP anti-mouse/human CD45R/B220 RA3-6B2 FC
PerCP/Cyanine5.5 anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 421™ anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F
Brilliant Violet 570™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 650™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 605™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 785™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 510™ anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F,SB
Purified anti-mouse/human CD45R/B220 (Maxpar® Ready) RA3-6B2 FC,CyTOF®
Brilliant Violet 711™ anti-mouse/human CD45R/B220 RA3-6B2 FC
PE/Dazzle™ 594 anti-mouse/human CD45R/B220 RA3-6B2 FC
APC/Fire™ 750 anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 750™ anti-mouse/human CD45R/B220 RA3-6B2 FC
TotalSeq™-A0103 anti-mouse/human CD45R/B220 RA3-6B2 PG
Spark Blue™ 550 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark NIR™ 685 anti-mouse/human CD45R/B220 RA3-6B2 FC
TotalSeq™-B0103 anti-mouse/human CD45R/B220 RA3-6B2 PG
Ultra-LEAF™ Purified anti-mouse/human CD45R/B220 RA3-6B2 FC,CyTOF®,IHC-F,IHC-P,IP,Activ
TotalSeq™-C0103 anti-mouse/human CD45R/B220 RA3-6B2 PG
PE/Fire™ 640 anti-mouse/human CD45R/B220 RA3-6B2 FC
APC/Fire™ 810 anti-mouse/human CD45R/B220 RA3-6B2 FC
PE/Fire™ 700 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark Violet™ 538 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark YG™ 581 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark YG™ 570 anti-mouse/human CD45R/B220 RA3-6B2 IHC-F
PE/Fire™ 810 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark Blue™ 574 anti-mouse/human CD45R/B220 Antibody RA3-6B2 FC
Spark Violet™ 423 anti-mouse/human CD45R/B220 Antibody RA3-6B2 FC
Spark Red™ 718 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark UV™ 387 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark PLUS UV395™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Go To Top Version: 3    Revision Date: 09/19/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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