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Purified anti-Aurora B Recombinant Antibody

Pricing & Availability
Clone
QA18A11 (See other available formats)
Regulatory Status
RUO
Other Names
Aurora kinase B, AURKB, Aurora 1, Aurora- and IPL1-like midbody-associated protein 1, Aurora/IPL1-related kinase 2 (ARK-2), STK-1, Protein Phosphatase 1, Regulatory Subunit 48, Serine/Threonine-Protein Kinase Aurora-B, Serine/Threonine-Protein
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
QA18A11_PURE_Aurora-B_Antibody_1_031120
Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-Aurora B recombinant antibody (clone QA18A11) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular weight marker. Cell lysates were loaded in order of ascending AURKB expression; predicted expression data was obtained from Human Protein Atlas.
  • QA18A11_PURE_Aurora-B_Antibody_1_031120
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-Aurora B recombinant antibody (clone QA18A11) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular weight marker. Cell lysates were loaded in order of ascending AURKB expression; predicted expression data was obtained from Human Protein Atlas.
  • QA18A11_PURE_Aurora-B_Antibody_2_031120
    HeLa cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with Triton X-100 for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with 5.0 µg/mL (1:100 dilution) of either purified rat IgG2a, κ isotype ctrl antibody (Cat. No. 400502) (panel A) or purified anti-Aurora B recombinant antibody (panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-rat IgG antibody (Cat. No. 405422) at 2.0 µg/mL. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective.
  • QA18A11_PURE_Aurora-B_Antibody_3_032320
    Whole cell extracts (250 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.5 µg of purified rat IgG2a, κ isotype ctrl antibody (Cat. No. 400502) or purified anti-Aurora B recombinant antibody (clone QA18A11). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1 µg/mL (1:500 dilution) of W18026A. Lane M: Molecular weight marker.
  • QA18A11_PURE_Aurora-B_Antibody_4_121322
    IHC staining of purified anti-Aurora B (clone QA18A11) on formalin-fixed paraffin-embedded human colon tissue. Following antigen retrieval using 1X Tris-Buffered Saline with Tween 20 (Cat. No. 925501), the tissue was incubated with blocking buffer (panel A) or 10 µg/mL of antibody overnight at 4°C (panel B), followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 goat anti-rat IgG (Cat. No. 405416) for one hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801), and the image was captured with a 40X objective. Scale bar: 50 µm
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936201 25 µg $101
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936202 100 µg $253
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Description

Aurora B, also known as AURKB, is a member of the Aurora family of Serine/Threonine protein kinases. The expression level of Aurora B is dramatically increased during the S/G2/M phases of the cell cycle. Aurora B plays a critical role in cell division and chromosome-microtubule interactions during mitosis. Aurora B forms the chromosomal passenger complex (CPC) together with INCENP, Survivin, and Borealin and functions to ensure faithful chromosome segregation. The CPC localizes to the kinetochores from prophase to metaphase and migrates to the central spindle and the midbody during telophase. Aurora B phosphorylates Histone H3 at residues Serine 53 and Serine 28 during prophase, leading to the dissociation of HP1 from chromatin and condensation of chromosome. Aurora B was found to be overexpressed in several malignant cancers and is involved in promoting tumor development and progression.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Rat
Immunogen
Partial recombinant human Aurora B protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IP, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 2.0 - 5.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone does not recognize mouse Aurora B.

This recombinant antibody clone is derived from clone W16153A.

RRID
AB_2832908 (BioLegend Cat. No. 936201)
AB_2832908 (BioLegend Cat. No. 936202)

Antigen Details

Structure
344 amino acids with a predicted molecular weight of 39.3 kD. Contains a Serine/Threonine kinase domain.
Distribution

Nucleus, chromosome, mitotic spindle, midbody

Function
Aurora B is a Serine/Threonine protein kinase belonging to the Aurora kinase family. It plays an important role in cell division by regulating mitosis and cytokinesis.
Interaction
Forms the chromosomal passenger complex (CPC) with INCENP, Survivin, and Borealin. Interacts with RACGAP1, CDCA1, EVI5, JTB, NDC80, PSMA3, SEPT1, SIRT2, TACC1, SPDYC, p53/TP53, NOC2L, TTC28, and RNF2/RING1B.
Biology Area
Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication, Chromatin Remodeling/Epigenetics, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase
Antigen References
  1. Abe Y, et al. 2016. Dev Cell. 36:487.
  2. Ritter A, et al. 2015. Cell Cycle. 14:3755.
  3. Park H, et al. 2014. Cell Cycle. 13:2391.
  4. Hegyi K & Méhes G. 2012. Pathol Oncol Res. 18:761.
  5. Carmena M, et al. 2009. Curr Opin Cell Biol. 21:796.
  6. Shannon KB, et al. 2002. Curr Biol. 12:R458.
Gene ID
9212 View all products for this Gene ID
UniProt
View information about Aurora B on UniProt.org
Go To Top Version: 3    Revision Date: 12/13/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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