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- Clone
- A15113A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Apolipoprotein J, Apo-J, Complement cytolysis inhibitor, Aging-associated gene 4 protein, Complement-associated protein SP-40,40, Ku70-binding protein 1, NA1/NA2 TRPM-2
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Western blot of anti-Clusterin antibody (clone A15113A) and isotype-matched IgG2a control, binding to endogenous and recombinant human clusterin. Lane 1: 0.5 µL of human serum; Lane 2: 50 ng of recombinant human clusterin protein. Blots were incubated with 5 µg/ml of each primary antibody at room temperature for one hour, followed by incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody (clone Poly4053). Enhanced chemiluminescence was used as the detection system. -
IHC staining of anti-Clusterin antibody (clone A15113A) on formalin-fixed paraffin-embedded brain tissue of a patient with Alzheimer's disease. Following antigen retrieval using Sodium Citrate H.I.E.R. and 0.25% Triton X-100, the tissue was incubated with the primary antibody at 5 µg/ml overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP Detection Kit was used for detection followed by hematoxylin counterstaining, according to the protocol provided.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 848701 | 25 µg | $101 | ||||
| 848702 | 100 µg | $229 | ||||
Clusterin, also known as Apolipoprotein J, is a heterodimeric glycoprotein that is a member of the heat-shock protein family. The protein exhibits a wide array of biological functions including clearance of cellular debris through chaperone activity, apoptosis, lipid transportation, cell clustering, and complement inhibition. Clusterin is involved in many diseases related to oxidative stress and aging, including neurodegenerative disorders, cancers, diabetes, and inflammatory diseases.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human clusterin.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P, Direct ELISA - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 2.0 - 10 µg/ml. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 2.0 - 10 µg/ml. For Direct ELISA, the suggested use of this reagent is 20 ng/ml - 3.0 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2650695 (BioLegend Cat. No. 848701)
AB_2650695 (BioLegend Cat. No. 848702)
Antigen Details
- Structure
- Clusterin is a protein of 449 amino acids with a molecular mass of 52 kD.
- Distribution
-
Tissue distribution: Ubiquitously expressed.
Cellular distribution: Primarily extracellular in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Intracellular clusterin is primarily present in the endoplasmic reticulum, cytosol, mitochondrion, and nucleus. - Function
- Clusterin is involved in several biological processes including extra- and intracellular chaperone activity, pro- and anti-apoptotic properties, lipid transportation, cell clustering, modulation of NF-kappa-B transcriptional activity, and complement inhibition.
- Interaction
- HDL.
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Complement, Neurodegeneration, Neuroinflammation, Neuroscience, Protein Misfolding and Aggregation
- Molecular Family
- Apolipoproteins
- Antigen References
-
1. Trougakos IP. 2013. Gerontology 59:514. PubMed
2. Rizzi F, Bettuzzi S. 2010. Endocr. Relat. Cancer 17:R1. PubMed
3. Desikan RS, et al. 2014. JAMA Neurol. 71:180. PubMed
4. Carver JA, et al. 2003. IUBMB Life. 55:661. PubMed - Gene ID
- 1191 View all products for this Gene ID
- UniProt
- View information about Clusterin on UniProt.org
Other Formats
View All Clusterin Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Clusterin | A15113A | WB,IHC-P,Direct ELISA |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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