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Purified anti-GAPDH Antibody

Pricing & Availability
Clone
W17079A (See other available formats)
Regulatory Status
RUO
Other Names
glyceraldehyde-3-phosphate dehydrogenase
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W17079A_PURE_GAPDH_Antibody_1_WB_042718_updated
Whole cell lysates (15 µg protein) from K562, PC3, HeLa, Molt-4, NTRA-2, NIH3T3, and UMR-106 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:10000 (0.05 µg/mL) Purified anti-GAPDH Antibody, clone W17079A. Proteins were visualized using chemiluminescence detection by incubating with 1:3000 dilution of HRP goat anti-rat-IgG secondary antibody (Cat. No. 405405, upper). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) was used as a loading control (lower). Lane M: MW ladder.
  • W17079A_PURE_GAPDH_Antibody_1_WB_042718_updated
    Whole cell lysates (15 µg protein) from K562, PC3, HeLa, Molt-4, NTRA-2, NIH3T3, and UMR-106 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:10000 (0.05 µg/mL) Purified anti-GAPDH Antibody, clone W17079A. Proteins were visualized using chemiluminescence detection by incubating with 1:3000 dilution of HRP goat anti-rat-IgG secondary antibody (Cat. No. 405405, upper). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) was used as a loading control (lower). Lane M: MW ladder.
  • W17079A_PURE_GAPDH_Antibody_2_ICC_042418
    HeLa cells were fixed with cold methanol for 15 minutes and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with (A) 1:500 (1 µg/ml) purified rat IgG2a, κ isotype control antibody (Cat. No. 400502) or 1:500 (1 µg/ml, B), 1:2000 (0.25 µg/ml, C) and 1:5000 (0.1 µg/ml, D) anti-GAPDH antibody (clone W17079A) overnight at 4°C followed by Alexa Fluor® 488 (green) conjugated goat anti-rat IgG (Cat. No. 405418) for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
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607901 25 µg $101
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607902 100 µg $253
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Description

GAPDH is well known for its glycolytic function of converting D-glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate. GAPDH is a ubiquitously expressed and has a molecular mass of 36 kD. Though differentially expressed from tissue to tissue, GAPDH is frequently used as a loading control for assays involving mRNA and protein detection. In more recent studies, GAPDH has been shown to be involved in microtubule bundling, prostate cancer progression, programmed neuronal cell death, DNA replication, and DNA repair. Recent work has elucidated roles for GAPDH in apoptosis, gene expression and nuclear transport. GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant protein (161-335 a.a.)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.0375-0.5 µg per ml (1:1000-1:16000 dilution). For immunocytochemistry, a concentration range of 0.25-1.0 μg/ml (1:500-1:2000 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone weakly reacts with rat GAPDH.

Product Citations
  1. Su T, et al. 2021. Metabolites. 11:. PubMed
  2. Nchioua R, et al. 2022. J Virol. 96:e0059422. PubMed
  3. Xie Q, et al. 2023. iScience. 26:106395. PubMed
  4. Qin XY, et al. 2023. Cell Death Dis. 14:358. PubMed
  5. Hayn M, et al. 2021. Cell Reports. 35(7):109126. PubMed
  6. Hirschenberger M, et al. 2021. STAR Protoc. 2:100781. PubMed
  7. Nchioua R, et al. 2020. MBio. 11:00. PubMed
  8. Srinivasachar Badarinarayan S, et al. 2020. Nucleic Acids Res. 48:10890. PubMed
  9. Prelli Bozzo C, et al. 2021. Nat Commun. 12:4584. PubMed
  10. Bosso M, et al. 2021. Cell Rep. 36:109735. PubMed
  11. Bosso M, et al. 2021. STAR Protoc. 2:100236. PubMed
  12. Bosso M, et al. 2020. PLoS Pathog. 16:e1008752. PubMed
  13. Simon Langer et al. 2019. eLife. 8 pii: e41930. PubMed
  14. Stanfield BA, et al. 2021. PLoS One. 16:e0245169. PubMed
  15. Nchioua R, et al. 2021. J Virol. 95:e0080821. PubMed
  16. Chamberlain N, et al. 2022. Int J Mol Sci. 23:. PubMed
  17. Chen HL, et al. 2021. Pharm Biol. 59:134. PubMed
  18. Viereckl MJ, et al. 2022. Biomolecules. 12:. PubMed
  19. Hopfensperger K, et al. 2020. MBio. 11:00. PubMed
  20. Huq S, et al. 2020. Mol Cancer Ther. 19:1797. PubMed
  21. Hayashi M, et al. 2021. Viruses. 13:. PubMed
  22. Zhao Z, et al. 2021. PLoS Pathog. 17:e1009728. PubMed
RRID
AB_2734503 (BioLegend Cat. No. 607901)
AB_2734503 (BioLegend Cat. No. 607902)

Antigen Details

Structure
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family, predicted molecular weight 36 kD. The enzyme exists as a tetramer of identical chains.
Distribution

Ubiquitously expressed, locate in cytoplasm and perinuclear region.

Function
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family, predicted molecular weight 36 kD. The enzyme exists as a tetramer of identical chains.
Interaction
Interacts with TPPP. Interacts with EIF1AD and WARS. Interacts with SUMO4, GLUT4, nPKC-iota and CAMK2.
Biology Area
Cell Biology, Western Blot Controls
Antigen References
  1. Ercolani L, et al. 1988. J. Biol. Chem. 263:15335.
  2. Meyer-Siegler K, et al. 1991. Proc. Natl. Acad. Sci. USA. 88:8460.
  3. Hara MR, Snyder SH. 2006. Cell. Mol. Neurobiol. 26:527.
  4. Zheng L, et al. 2003. Cell. 114:255.
  5. Wang Q, et al. 2005. FASEB J. 19:869.
  6. Bae BI, et al. 2006. Proc. Natl. Acad. Sci. USA. 103:3405.
Gene ID
5590 View all products for this Gene ID
UniProt
View information about GAPDH on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 04/27/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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