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PE anti-mouse CD49e Antibody

Pricing & Availability
Clone
5H10-27(MFR5) (See other available formats)
Regulatory Status
RUO
Other Names
α5 integrin, VLA-5 α chain, Integrin α5, Fnra, Itga5
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
5h10-27
C57BL/6 mouse bone marrow cells stained with 5H10-27 PE
  • 5h10-27
    C57BL/6 mouse bone marrow cells stained with 5H10-27 PE
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103805 50 µg 104€
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Description

CD49e is a 135 kD protein, also known as α5 integrin or VLA-5 α chain. It is a member of the integrin family, expressed on thymocytes, splenic B cells, activated T cells, and mast cells. CD49e associates with CD29 (integrin β1 chain) to form the fibronectin receptor (VLA-5). CD49e plays a critical role in both adhesion and T cell costimulation. The primary ligand for CD49e/CD29 (VLA-5) is fibronectin. The 5H10-27(MFR5) antibody has been shown to block CD49e mediated interactions and promote the in vitro stimulation of CD8+ T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
(C57BL/6 x A/J)F1 mouse mast cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining6,7 of acetone-fixed frozen sections, blocking of cell-cell adhesion1-5, inhibition of TNF-ß1 costimulated T cell proliferation3, and costimulation of T cell proliferation by cross-linked 5H10-27 antibody3. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103817 and 103818).

Application References
  1. Kinashi T, et al. 1994. Blood Cells 20:25. (Block)
  2. Halvorson MJ, et al. 1995. J. Immunol. 155:4567. (Block)
  3. Rich S, et al. 1996. J. Immunol. 157:2916. (Block Costim))
  4. Uhlenkott CE, et al. 1996. Clin. Exp. Metastasis 14:125. (Block)
  5. Schultz JF, et al. 1995. J. Biol. Chem. 270:11522. (Block)
  6. Yang JT, et al. 1996. Mol. Biol. Cell. 7:1737. (IHC)
  7. Cuklerman E, et al. 2001. Science 294:1708. (IHC)
  8. Jia W, et al. 2005. Blood 106:3854. (FC)
Product Citations
  1. Bultmann–Mellin I, et al. 2017. Am J Physiol Lung Cell Mol Physiol. 313:L687. PubMed
  2. Nording H, et al. 2021. Nat Commun. 12:3352. PubMed
  3. Horiguchi H, et al. 2019. Genes Dev. 33:1641. PubMed
  4. Yokota T, et al. 2020. Cell. 182(3):545-562.e23. PubMed
RRID
AB_313054 (BioLegend Cat. No. 103805)

Antigen Details

Structure
Integrin family, associates with integrin β1, 135 kD
Distribution

Thymocytes, activated T cells, mast cells

Function
Adhesion, costimulation
Ligand/Receptor
Fibronectin
Cell Type
Mast cells, Mesenchymal Stem Cells, T cells, Thymocytes, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kinashi T, et al. 1994 Blood Cells 20:25.
3. Hemler ME. 1990. Annu. Rev. Immunol. 8:365.

Gene ID
16402 View all products for this Gene ID
UniProt
View information about CD49e on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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