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PE/Cyanine5 anti-human CD18 Antibody

Pricing & Availability
Clone
TS1/18 (See other available formats)
Regulatory Status
RUO
Workshop
V AS162
Other Names
Integrin β2 subunit, LFA -1 β subunit, β2 integrin, ITGB2
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
TS1slash18_PECy5_061807
Human peripheral blood lymphocytes stained with TS1/18 PE/Cyanine5
  • TS1slash18_PECy5_061807
    Human peripheral blood lymphocytes stained with TS1/18 PE/Cyanine5
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302109 25 tests 104€
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Description

CD18 is a 90-95 kD type I transmembrane protein also known as integrin β2 subunit, LFA-1 β subunit, and β2 integrin. CD18 non-covalently associates with CD11a, CD11b or CD11c. CD18 is expressed on all leukocytes. CD18 and associated α chains function in adhesion and signaling in hematopoietic cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Pig
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: inhibition of cell adhesion and migration3,4. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 302116) is recommended for functional assays.

Application References
  1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Kishimoto T, et al. 1997. Leucocyte Typing VI. Garland Press. London.
  3. Van Epps DE, et al. 1989. J. Immunol. 143:3207. (Block)
  4. Meerschaert J, et al. 1994. J. Immunol. 152:1915. (Block)
  5. Sithu SD, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M611273200.
  6. Sommaggio R, et al. 2012. J. Immunol. 188:2075. PubMed
  7. Valenzuela NM, et al. 2013. J. Immunol. 190:6635. PubMed
Product Citations
  1. McKillop W, et al. 2009. J Leukoc Biol. 86:851. PubMed
  2. Mastrogiovanni M, et al. 2022. Sci Adv. 8:eabl5942. PubMed
  3. Hegewisch-Solloa E, et al. 2021. J Immunol. 207:950. PubMed
  4. Schmidt T, et al. 2012. J Leukoc Biol. 91:791. PubMed
  5. Navarro-Hernandez IC, et al. 2020. FEBS J. 287:3449. PubMed
RRID
AB_314227 (BioLegend Cat. No. 302109)

Antigen Details

Structure
Integrin, type I transmembrane glycoprotein, associates with CD11a, CD11b, or CD11c, 90-95 kD
Distribution

Leukocytes

Function
Adhesion and signaling in hematopoietic system
Ligand/Receptor
ICAMs
Cell Type
Leukocytes, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Anderson D, et al. 1987. Annu. Rev. Med. 38:175.
2. Springer T. 1994. Cell 76:301.

Gene ID
3689 View all products for this Gene ID
UniProt
View information about CD18 on UniProt.org
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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