- Clone
- LM2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Integrin αM chain, C3biR, CR3, Mac-1, Mo1, ITGAM
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![LM2_PerCPCyanine55_CD11b_Antibody_060519_updated.png LM2_PerCPCyanine55_CD11b_Antibody_060519_updated.png](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/LM2_PerCPCyanine55_CD11b_Antibody_060519_updated.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes, monocytes, and granulocytes were stained with True-Stain Monocyte Blocker™ (Cat. No. 426103) and CD11b (clone LM2) PerCP/Cyanine5.5 (left) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (right).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
393105 | 25 tests | 100€ | ||||
393106 | 100 tests | 278€ |
CD11b is a 165-170 kD type I transmembrane glycoprotein also known as αM integrin, Mac-1, CR3, and C3biR. CD11b non-covalently associates with integrin β2 (CD18) and is expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b/CD18 is critical for the transendothelial migration of monocytes and neutrophils. It is also involved in granulocyte adhesion, phagocytosis, and neutrophil activation. CD11b/CD18 interacts with ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, and factor X.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- β2 integrins from granulocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. - Excitation Laser
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Blue Laser (488 nm)
- Application Notes
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LM2 does not block clone ICRF44 indicating a unique epitope.
- Additional Product Notes
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BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Product Citations
-
- RRID
-
AB_2734452 (BioLegend Cat. No. 393105)
AB_2734453 (BioLegend Cat. No. 393106)
Antigen Details
- Structure
- Integrin, type I transmembrane glycoprotein, associates with integrin β2 (CD18), 165-170 kD
- Distribution
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Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subset of T cells, subset of B cells
- Function
- Adhesion, phagocytosis, chemotaxis, neutrophil activation
- Ligand/Receptor
- ICAM-1(CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, factor X
- Cell Type
- B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils, NK cells, T cells
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
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- Stewart M, et al. 1995. Curr Opin Cell Biol. 7:690.
- Miller LJ, et al. 1986. J Immunol. 137(9):2891.
- Gene ID
- 3684 View all products for this Gene ID
- UniProt
- View information about CD11b on UniProt.org
Related FAQs
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
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PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
Other Formats
View All CD11b Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD11b | LM2 | FC |
Alexa Fluor® 647 anti-human CD11b | LM2 | FC |
Alexa Fluor® 488 anti-human CD11b | LM2 | FC |
PerCP/Cyanine5.5 anti-human CD11b | LM2 | FC |
PE/Cyanine7 anti-human CD11b | LM2 | FC |
Brilliant Violet 421™ anti-human CD11b | LM2 | FC |
PE anti-human CD11b | LM2 | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD11b
Human peripheral blood lymphocytes, monocytes, and granulocy... -
Alexa Fluor® 647 anti-human CD11b
Human peripheral blood lymphocytes, monocytes, and granulocy... -
Alexa Fluor® 488 anti-human CD11b
Human peripheral blood lymphocytes, monocytes, and granulocy... -
PerCP/Cyanine5.5 anti-human CD11b
Human peripheral blood lymphocytes, monocytes, and granulocy... -
PE/Cyanine7 anti-human CD11b
Human peripheral blood lymphocytes, monocytes, and granulocy... -
Brilliant Violet 421™ anti-human CD11b
Human peripheral blood lymphocytes, monocytes, and granulocy... -
PE anti-human CD11b
Human peripheral blood lymphocytes, monocytes, and granulocy...
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