Purified anti-mouse CD11a/CD18 LFA-1 Antibody anti-CD11a/CD18

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Clone: H155-78 (See other available formats)
Regulatory Status: RUO
Other Names: Lymphocyte function-associated antigen 1, ITGAL, ITGB2
Isotype: Rat IgG1, κ
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Product Citations: publications

H155-78_PerCP_Cyanine5.5_120210 — C57BL/6 splenocytes were stained with LFA-1 (clone H155-78) PerCP/Cyanine5.5 (filled histogram) or rat IgG1 PerCP/Cyanine5.5 isotype control (open histogram).

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141002

500 µg

184€

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Description

Lymphocyte function-associated antigen 1 or LFA-1 is a heterodimer composed of two members of the integrin family, α_L (CD11a) and β2 (CD18). LFA-1 is expressed primarily on lymphocytes, monocytes/macrophages, and granulocytes. It is involved in cell adhesion and costimulation processes. The ligands for LFA-1 are ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50).

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: T_H-derived anti-I-A^k CTL clone A1 5.1.17
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone is reported to have some effect on increasing LFA-1-mediated adhesion and does not block LFA-1 binding¹.
RRID: AB_10694866 (BioLegend Cat. No. 141002)

Antigen Details

Structure: Heterodimer composed of two members of the integrin family, &alpha_L (CD11a) and β2 (CD18).
Distribution: Primarily expressed on lymphocytes, monocytes/macrophages, and granulocytes
Function: Intercellular adhesion and costimulation
Ligand/Receptor: ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), and ICAM-3 (CD242)
Cell Type: B cells, Dendritic cells, Lymphocytes, Macrophages, Monocytes
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Signal Transduction
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Dugger KJ, et al. 2009. J. Neuroimmunol. 206:22.
2. Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press.
3. Springer TA. 1994. Cell 76:301.
4. Lub M, et al. 1995. Immunol. Today 16:479.
Gene ID: 16408 View all products for this Gene ID 16414 View all products for this Gene ID
UniProt: View information about CD11a CD18 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

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Pathways

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Other Formats

View All CD11a/CD18 Reagents Request Custom Conjugation

Description	Clone	Applications
PerCP/Cyanine5.5 anti-mouse CD11a/CD18 (LFA-1)	H155-78	FC
Purified anti-mouse CD11a/CD18 (LFA-1)	H155-78	FC
PE anti-mouse CD11a/CD18 (LFA-1)	H155-78	FC
APC anti-mouse CD11a/CD18 (LFA-1)	H155-78	FC
PE/Cyanine7 anti-mouse CD11a/CD18 (LFA-1)	H155-78	FC
Pacific Blue™ anti-mouse CD11a/CD18 (LFA-1)	H155-78	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

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