Brilliant Violet 421™ anti-mouse TCR β chain Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

Affinity purified TCR from mouse DO-11.10 cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.

Concentration

µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For immunofluorescent staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

H57-597 is a hamster mAb directed to an epitope of the C region of TCR ß chain¹². The H57-597 antibody does not cross-react with ?/d TCR-bearing T cells. Immobilized or soluble H57-597 antibody can activate a/ß TCR-bearing T cells. Additional reported applications (for the relevant formats) for this antibody include: immunoprecipitation², in vitro stimulation^2,3, in vivo depletion^4-6, and immunohistochemical staining of acetone-fixed frozen sections^7,8,9. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 109253-109258).

Application References

(PubMed link indicates BioLegend citation)

1. Gascoigne NJ. 1990. J. Biol. Chem. 265:9296.
2. Kruisbeek A, et al. 1991. In Current Protocols in Immunology. pp. 3.12.1. (Costim IP)
3. Davenport C, et al. 1995. J. Immunol. 155:3742. (Costim)
4. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
5. Kummer U, et al. 2001. Immunol. Lett. 75:153. (Deplete)
6. van der Heyde HC, et al. 1995. J. Immunol. 154:3985. (Deplete)
7. Tomita K, et al. 1999. Genes Dev. 13:1203. (IHC)
8. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
9. Ponomarev ED, et al. 2007. J. Immunol. 178:39. (IHC)
10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
11. Tsukumo S, et al. 2006. J.Immunol. 177:8365. (FC) PubMed
12. Grégoire C, et al. 1991. Proc. Natl. Acad. Sci USA 88:8077.

Product Citations

1. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
2. Soon MSF, et al. 2020. Nat Immunol. 1.984027778. PubMed
3. Lee SA, et al. 2020. J Immunol. 204:586. PubMed
4. Campisi L, et al. 2022. Nature. 606:945. PubMed
5. Glaubitz J, et al. 2023. Gut. :. PubMed
6. Shibata K, et al. 2022. Nat Commun. 13:6948. PubMed
7. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
8. Michelet X, et al. 2015. J Immunol. 194:2079. PubMed
9. Donado CA, et al. 2020. Cell Reports. 31(1):107466. PubMed
10. Park JG, et al. 2021. iScience. 24(9):102941. PubMed
11. Yang W, et al. 2020. Nat Commun. 3.553472222. PubMed
12. Tyagi AM et al. 2018. Immunity. 49(6):1116-1131 . PubMed
13. Chen S et al. 2018. Cell reports. 25(7):1729-1740 . PubMed
14. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
15. Gómez-Díaz C, et al. 2021. iScience. 24:103241. PubMed
16. Wiede F, et al. 2021. Cancer Discov. Online ahead of print. PubMed
17. Yu M, et al. 2021. J Clin Invest. 131:. PubMed
18. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
19. Sun Y, et al. 2022. Nat Commun. 13:3916. PubMed
20. Canton J, et al. 2021. Nat Immunol. 22:140. PubMed
21. Prosser A, et al. 2021. Cell Reports. 35(7):109141. PubMed
22. Kawakami R, et al. 2021. Immunity. 54(5):947-961.e8. PubMed
23. Romano A, et al. 2015. J Immunol. 195: 3816 - 3827. PubMed
24. Jackson-Jones LH, et al. 2020. Immunity. 52:700. PubMed
25. Tyagi AM, et al. 2021. eLife. 10:00. PubMed
26. Fennell LM, et al. 2020. EMBO J. 39:e103303. PubMed
27. Lu X, et al. 2020. Sci Transl Med. 12:. PubMed
28. Watanabe M, et al. 2020. Nat Commun. 4.808333333. PubMed
29. Goh PK, et al. 2022. Sci Adv. 8:eabk3338. PubMed
30. Nagatake T, et al. 2018. Int Immunol. 30:471. PubMed
31. Xi‐Zhi J Guo et al. 2018. Immunity. 49(3):531-544 . PubMed
32. Xiao S, et al. 2020. Cell Reports. 32(2):107892. PubMed
33. Jandke A, et al. 2020. Nat Commun. 3.075694444. PubMed
34. Peters N, et al. 2014. PLoS Pathog. 10:1004538. PubMed
35. Nagatake T, et al. 2018. J Allergy Clin Immunol. 142:470. PubMed
36. Oliveira AC et al. 2017. eLife. 6 pii: e30883. PubMed
37. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
38. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
39. Wei Y, et al. 2021. Endocrinology. 162(8):. PubMed
40. Muri J, et al. 2020. Cell Reports. 29(9):2731-2744.e4.. PubMed
41. Boulch M, et al. 2021. Sci Immunol. 6:. PubMed
42. Iwanami N, et al. 2020. iScience. 23:101260. PubMed
43. Ito Y, et al. 2021. Cell Reports. 35(4):109052. PubMed
44. Trivedi S, et al. 2020. Elife. 9:00. PubMed

RRID

AB_10933263 (BioLegend Cat. No. 109229)
AB_10933263 (BioLegend Cat. No. 109230)

Antigen Details

Structure

Ig superfamily, CD3/TCR complex with CD3 and TCR α subunit

Distribution

Majority of T cells and thymocytes (correlated to differentiation)

Function

Antigen recognition, T cell activation

Ligand/Receptor

Peptide bound-MHC class I and II

Antigen References

1. Davis MM, et al. 1998. Ann. Rev. Immunol. 16:523.
2. Huppa JB, et al. 2003. Nat. Immunol. 4:749.
3. Kubo R, et al. 1989. J. Immunol. 142:2736.

Gene ID

21577 View all products for this Gene ID

Specificity (DOES NOT SHOW ON TDS):

TCR beta chain

Specificity Alt (DOES NOT SHOW ON TDS):

TCR β chain

App Abbreviation (DOES NOT SHOW ON TDS):

FC,IHC-F

UniProt

View information about TCR beta chain on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Other Formats

Certificate of Analysis