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FITC anti-human CD14 Antibody

Pricing & Availability
Clone
63D3 (See other available formats)
Regulatory Status
RUO
Other Names
Monocyte differentiation antigen CD14, myeloid cell-specific leucine-rich glycoprotein, LPS receptor
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
63D3_FITC_CD14_Antibody_021216
Human peripheral blood monocytes were stained with FITC anti-human CD14 (clone 63D3) (filled histogram) or mouse IgG1, κ FITC isotype control (open histogram).
  • 63D3_FITC_CD14_Antibody_021216
    Human peripheral blood monocytes were stained with FITC anti-human CD14 (clone 63D3) (filled histogram) or mouse IgG1, κ FITC isotype control (open histogram).
Compare all formats See FITC spectral data
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367115 25 tests 89 CHF
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367116 100 tests 206 CHF
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Description

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein that is also known as the LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokine expression in monocytes and neutrophils.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified human peripheral blood monocytes.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Fridlender ZG, et al. 1999. Hum. Immunol. 11:1028.
  2. Devitt A, et al. 1998. Nature 6675:505.
Product Citations
  1. Singh VK, et al. 2022. Front Immunol. 13:865503. PubMed
  2. Jiao Q, et al. 2020. Br J Dermatol. 182:648. PubMed
  3. Yusenko MV, et al. 2021. Cancers (Basel). 14: . PubMed
  4. Gomez-Salinero JM, et al. 2022. Cell Stem Cell. 29:593. PubMed
  5. Baudet A, et al. 2022. Bio Protoc. 12:e4353. PubMed
  6. Ji K, et al. 2023. Clin Transl Med. 13:e1234. PubMed
  7. He XP, et al. 2018. Mol Med Rep. 3.6125. PubMed
  8. Lauterbach MA, et al. 2020. Immunity. 51(6):997-1011.e7.. PubMed
  9. Taura M, et al. 2019. Proc Natl Acad Sci U S A. 116:2282. PubMed
  10. Li J, et al. 2021. Front Immunol. 12:649285. PubMed
  11. Zhang X, et al. 2021. Front Immunol. 12:680068. PubMed
  12. Pellegrini JM, et al. 2021. Sci Rep. 11:13559. PubMed
  13. Fitzgerald KC, et al. 2021. Cell Rep Med. 2:100424. PubMed
  14. Liu T, et al. 2022. Front Immunol. 13:901349. PubMed
  15. Qing P, et al. 2020. Exp Physiol. 1708:105. PubMed
  16. Besnier M, et al. 2021. Antioxidants (Basel). 10:. PubMed
  17. Wendisch D, et al. 2021. Cell. 184:6243. PubMed
  18. Su S et al. 2018. Cell. 175(2):442-457 . PubMed
  19. Lv J, et al. 2021. Mol Med Rep. 1. PubMed
  20. Yan W, et al. 2020. Biology of Reproduction. . PubMed
  21. Motedayyen H, et al. 2017. BMC Res Notes. . 10.1186/s13104-017-2880-6. PubMed
  22. He Z, et al. 2020. J Exp Clin Cancer Res. 39:140. PubMed
  23. Hu X, et al. 2020. Neoplasia. 1.290972222. PubMed
  24. Lavaert M, et al. 2020. Frontiers in Immunology. 1.610416667. PubMed
  25. Koh WH, et al. 2020. STAR Protoc. 1:100203. PubMed
  26. Sullivan KD et al. 2016. eLife. 5 pii: e16220. PubMed
  27. Vanoni G, et al. 2021. eLife. 10:00. PubMed
  28. Gorby C, et al. 2020. Sci Signal. :13. PubMed
  29. Kang HM, et al. 2018. Nat Biotechnol. 36:89. PubMed
RRID
AB_2571928 (BioLegend Cat. No. 367115)
AB_2571928 (BioLegend Cat. No. 367116)

Antigen Details

Structure
GPI-linked membrane glycoprotein.
Distribution

Monocytes, macrophages, dendritic cells, and granulocytes.

Function
LPS receptor, clearance of Gram-negative pathogens.
Interaction
LPS.
Ligand/Receptor
LPS.
Cell Type
Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Stocks SC, et al. 1990. Biochem. J. 268:275.
  2. Wright SD, et al. 1990. Science 4975:1431.
Gene ID
929 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD14
Specificity Alt (DOES NOT SHOW ON TDS):
CD14
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD14 on UniProt.org
Go To Top Version: 2    Revision Date: 11.14.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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