Loading...

FITC anti-human CD3 Antibody

Pricing & Availability
Clone
UCHT1 (See other available formats)
Regulatory Status
RUO
Workshop
III 471
Other Names
T3, CD3ε
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
UCHT1_FITC_021805
Human peripheral blood lymphocytes stained with UCHT1 FITC
  • UCHT1_FITC_021805
    Human peripheral blood lymphocytes stained with UCHT1 FITC
Compare all formats See FITC spectral data
Cat # Size Price Quantity Check Availability Save
300405 25 tests 22 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
300406 100 tests 50 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
300452 100 µg 54 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
300440 500 tests 204 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NKT cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
µg sizes: 0.5 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, Western blotting9, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP)
  2. Graves J, et al. 1991. J. Immunol. 146:2102. (Activ)
  3. Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)
  4. Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)
  5. Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)
  6. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC)
  7. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  8. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  9. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
  10. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  11. Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC)
  12. Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed
  13. Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed
  14. Li J, et al. 2015. Cancer Res. 75:508. PubMed
  15. Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG)
  16. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Lin L, et al. 2020. NAR Genom Bioinform. 2:lqaa059. PubMed
  2. Carre C, et al. 2021. iScience. 24:102970. PubMed
  3. Qin L, et al. 2022. Sci Adv. 8:eabn3774. PubMed
  4. Gan X, et al. 2022. Proc Natl Acad Sci U S A. 119:e2200879119. PubMed
  5. Sušac L, et al. 2022. Cell. 185:3201. PubMed
  6. Saber MM, et al. 2022. J Immunol Res. 2022:7219207. PubMed
  7. McEvoy CM, et al. 2022. Nat Commun. 13:7634. PubMed
  8. Graciliano NG, et al. 2023. Front Immunol. 13:1031248. PubMed
  9. Sun W, et al. 2023. Nature. 614:309. PubMed
  10. Namvar N, et al. 2022. Iran J Allergy Asthma Immunol. 21:600. PubMed
  11. Zhong J, et al. 2023. Front Immunol. 13:1081889. PubMed
  12. Yen M, et al. 2022. Cell. 185:1414. PubMed
  13. Cao B, et al. 2022. Nat Commun. 13:6203. PubMed
  14. Soday L, et al. 2021. Frontiers in Immunology. 12:600056. PubMed
  15. Johnson DC, et al. 2018. Nat Med. 24:1151. PubMed
  16. Sajadi MM, et al. 2018. Cell. 173:1783. PubMed
  17. Jiang G, et al. 2020. Aging (Albany NY). 12:11466. PubMed
  18. Bhattacharya S, et al. 2020. Front Bioeng Biotechnol. 1.055555556. PubMed
  19. Shang P, et al. 2022. Acta Biochim Biophys Sin (Shanghai). 54:1497. PubMed
  20. Porbahaie M, et al. 2023. PLoS One. 18:e0279626. PubMed
  21. Li H, et al. 2023. Clin Rheumatol. 42:1327. PubMed
  22. Freund EC, et al. 2023. Methods Mol Biol. 2618:201. PubMed
  23. Gao F, et al. 2023. Immunity. 56:864. PubMed
  24. Lütge M, et al. 2023. Nat Immunol. . PubMed
  25. Imai H, et al. 2023. iScience. 26:106822. PubMed
  26. Patskovsky Y, et al. 2023. Nat Commun. 14:3763. PubMed
  27. Ye Y, et al. 2022. Nat Commun. 13:6458. PubMed
  28. Li C, et al. 2022. J Exp Med. 219:. PubMed
  29. Netsrithong R, et al. 2020. Stem Cell Research. 39:101487.. PubMed
  30. Rendeiro AF, et al. 2020. Nat Commun. 11:577. PubMed
  31. Ryzhov S, et al. 2019. J Am Heart Assoc. 8:e010874. PubMed
  32. Barrow AD et al. 2018. Cell. 172(3):534-548 . PubMed
  33. Klemm F, et al. 2020. Cell. 181(7):1643-1660.e17. PubMed
  34. Kimura I, et al. 2022. Cell Rep. 38:110218. PubMed
  35. Chen M, et al. 2021. Cancers (Basel). 13:. PubMed
  36. Laustsen A, et al. 2021. Elife. 10:. PubMed
  37. Pholcharee T, et al. 2021. Nat Commun. 12:1063. PubMed
  38. Rolandelli A, et al. 2017. Sci Rep. 7:40666. PubMed
  39. Karlsson H, et al. 2015. PLoS One. 10: 0144787. PubMed
  40. Chen L, et al. 2018. Immunology. 155:123. PubMed
  41. Liu C, et al. 2021. Cell Res. 31:1106. PubMed
  42. Devalraju KP, et al. 2021. PLoS One. 16:e0257185. PubMed
  43. Ruetsche D, et al. 2022. Cells. 11:. PubMed
  44. Esaulova E, et al. 2020. Cell Host Microbe. 29(2):165-178.e8. PubMed
  45. Eri Yamada et al. 2018. Cell host & microbe. 23(1):110-120 . PubMed
  46. Zhan Y, et al. 2021. JCI Insight. 6:. PubMed
  47. Engelmann C, et al. 2017. J Transl Med. 10.1186/s12967-017-1288-3. PubMed
  48. Perelman SS, et al. 2021. Nat Microbiol. 6:731. PubMed
  49. Zirbes A, et al. 2021. J Mammary Gland Biol Neoplasia. 26:247. PubMed
  50. Jiang Y, et al. 2020. Clin Immunol. 218:108516. PubMed
  51. Bergamaschi L, et al. 2021. Immunity. 54(6):1257-1275.e8. PubMed
  52. Appios A, et al. 2021. Bio Protoc. 11:e4044. PubMed
  53. Yarmarkovich M, et al. 2021. Nature. 599:477. PubMed
  54. Shevyrev D, et al. 2021. Exp Ther Med. 209:21. PubMed
  55. Caduff N, et al. 2021. Cell Reports. 35(5):109056. PubMed
  56. Ping Y, et al. 2020. Front Cell Dev Biol. 0.890972222. PubMed
  57. Maritz MF, et al. 2018. PLoS One. 13:e0196043. PubMed
  58. Tu HA, et al. 2020. Cell Rep Med. 1:100155. PubMed
  59. Ramos RN, et al. 2022. Clin Transl Immunology. 11:e1392. PubMed
  60. Yuan Z, et al. 2018. Emerg Microbes Infect. 7:59. PubMed
  61. Rajamanickam V, et al. 2021. Cancer Immunol Res. 9:602. PubMed
  62. Laroni A, et al. 2016. J Autoimmun. 72:8-18. PubMed
  63. Corleis B, et al. 2017. PLoS One. 12:e0173161. PubMed
  64. Ray RM, et al. 2019. Sci Rep. 9:4334. PubMed
  65. Sawasdee N, et al. 2022. Int J Mol Med. 49:. PubMed
  66. Mishra HK, et al. 2021. Front Immunol. 12:711621. PubMed
  67. Minervina AA, et al. 2022. Nat Immunol. 23:781. PubMed
  68. Cai D, et al. 2020. Cell Mol Immunol. 17:227. PubMed
  69. Krishnan S, et al. 2021. Clin Exp Immunol. 203:458. PubMed
  70. Rossignol ED, et al. 2021. Cell Reports. 35(8):109167. PubMed
  71. Huang N, et al. 2020. Genome Biol. 1.03125. PubMed
  72. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  73. Platten M, et al. 2021. Nature. 592:463. PubMed
  74. Jin S, et al. 2020. Cell Res. 30:950. PubMed
  75. Liu Y, et al. 2020. Methods Mol Biol. 2111:285. PubMed
  76. Kariminia A, et al. 2016. Blood. 127: 3082 - 3091. PubMed
  77. Lima J, et al. 2016. Reprod Sci. 10.1177/1933719116653680. PubMed
  78. Meermeier E, et al. 2016. Nat Commun. 7:12506. PubMed
  79. Whitfield SJC, et al. 2017. J Immunol. 198:3989. PubMed
  80. Cizmeci D, et al. 2021. eLife. 10:00. PubMed
  81. Coufal S, et al. 2019. Cells. 8. PubMed
  82. Wang J, et al. 2019. Oncol Lett. 4.948611111. PubMed
  83. Kato M, et al. 2021. PLoS One. 16:e0252116. PubMed
  84. Bak S, et al. 2019. Front Immunol. 9:2953. PubMed
  85. Shin CH, et al. 2020. J Immunol. 205:3179. PubMed
  86. Hakki S, et al. 2022. Sci Rep. 12:1427. PubMed
  87. Cecotto L, et al. 2022. iScience. 25:105211. PubMed
  88. Setliff I, et al. 2018. Cell Host Microbe. 23:845. PubMed
  89. Collins PL et al. 2018. Cell. 176(1-2):348-360 . PubMed
  90. Reuschl AK, et al. 2022. Cell Rep. 39:110650. PubMed
  91. Abe Y, et al. 2022. Nat Cell Biol. 24:565. PubMed
  92. Cipriano M, et al. 2022. Commun Biol. 5:52. PubMed
  93. Kaito Y, et al. 2022. Oncol Lett. 23:51. PubMed
  94. Wang Y, et al. 2021. Sci Adv. 7: . PubMed
  95. Cantoni C, et al. 2021. Oncoimmunology. 1879530:10. PubMed
  96. Motozono C, et al. 2021. Cell Host Microbe. . PubMed
  97. Chapelin F, et al. 2017. Sci Rep.. 10.1038/s41598-017-17669-4. PubMed
  98. Duhen R, et al. 2021. Nat Commun. 12:1047. PubMed
  99. Wang Z, et al. 2021. Nat Commun. 12:1724. PubMed
  100. Capone S, et al. 2020. NPJ Vaccines. 5:94. PubMed
  101. Domanska D, et al. 2022. J Exp Med. 219:. PubMed
  102. van Erp EA, et al. 2020. Clin Transl Immunology. 9:e1112. PubMed
  103. Maas RR, et al. 2021. Nat Protoc. 16:4692. PubMed
  104. Tamai Y, et al. 2013. J Immunol. 190:4382. PubMed
  105. Nakatsugawa M, et al. 2015. J Immunol. 194 3487 . PubMed
  106. Nakatsugawa M, et al. 2016. Sci Rep. 6:23821. PubMed
  107. Lee M, et al. 2022. Front Cell Dev Biol. 10:909557. PubMed
  108. Albrecht M, et al. 2022. Clin Transl Immunology. 11:e1398. PubMed
  109. Rambaldi B, et al. 2022. Haematologica. 107:2617. PubMed
  110. Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
  111. Chen YP, et al. 2020. Cell Res. 30:1024. PubMed
  112. Dyson MR, et al. 2020. MAbs. 12:1829335. PubMed
  113. Ochi T, et al. 2015. Cancer Immunol Res. 3: 1070-1081. PubMed
  114. Suzuki D, et al. 2020. Stem Cell Reports. 14:49. PubMed
  115. Beyer AI, et al. 2017. Stem Cells Dev. 26:102. PubMed
  116. Wang SQ, et al. 2021. J Viral Hepat. 28:795. PubMed
  117. Pappa V, et al. 2021. J Neuroimmunol. 353:577524. PubMed
  118. Vanoni G, et al. 2021. eLife. 10:00. PubMed
  119. Goc J, et al. 2021. Cell. 184:5015. PubMed
  120. Wang S, et al. 2022. Cancer Immunol Immunother. Online ahead of print. PubMed
  121. Schmidts A, et al. 2020. J Immunother Cancer. 8:. PubMed
  122. Bennstein SB, et al. 2020. eLife. 9:e55232.. PubMed
  123. Chang ZL, et al. 2020. Nature Protocols. 15(4):1507-1524.. PubMed
RRID
AB_2562046 (BioLegend Cat. No. 300405)
AB_2562046 (BioLegend Cat. No. 300406)
AB_2562046 (BioLegend Cat. No. 300452)
AB_2562046 (BioLegend Cat. No. 300440)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD3
Specificity Alt (DOES NOT SHOW ON TDS):
CD3
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD3 on UniProt.org

Other Formats

View All CD3 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD3 UCHT1 FC
Biotin anti-human CD3 UCHT1 FC
FITC anti-human CD3 UCHT1 FC
PE anti-human CD3 UCHT1 FC
PE/Cyanine5 anti-human CD3 UCHT1 FC
Purified anti-human CD3 UCHT1 FC,CyTOF®,IHC-F,IP,Activ,WB
Alexa Fluor® 647 anti-human CD3 UCHT1 FC,ICC,IHC-F
Alexa Fluor® 488 anti-human CD3 UCHT1 FC,ICC,IHC-F
Pacific Blue™ anti-human CD3 UCHT1 FC
PE/Cyanine7 anti-human CD3 UCHT1 FC
Alexa Fluor® 700 anti-human CD3 UCHT1 FC
APC/Cyanine7 anti-human CD3 UCHT1 FC
PerCP anti-human CD3 UCHT1 FC
PerCP/Cyanine5.5 anti-human CD3 UCHT1 FC
Brilliant Violet 421™ anti-human CD3 UCHT1 FC,ICC,IHC-F
Brilliant Violet 570™ anti-human CD3 UCHT1 FC
Ultra-LEAF™ Purified anti-human CD3 UCHT1 FC,CyTOF®,IHC-F,IP,Activ,WB
Purified anti-human CD3 (Maxpar® Ready) UCHT1 FC,CyTOF®
Alexa Fluor® 594 anti-human CD3 UCHT1 ICC,FC,SB
PE/Dazzle™ 594 anti-human CD3 UCHT1 FC
Brilliant Violet 510™ anti-human CD3 UCHT1 FC
Brilliant Violet 605™ anti-human CD3 UCHT1 FC
Brilliant Violet 711™ anti-human CD3 UCHT1 FC
Brilliant Violet 650™ anti-human CD3 UCHT1 FC
APC/Fire™ 750 anti-human CD3 UCHT1 FC
Pacific Blue™ anti-human CD3 UCHT1 FC
Brilliant Violet 785™ anti-human CD3 UCHT1 FC
PE/Dazzle™ 594 anti-human CD3 UCHT1 FC
TotalSeq™-A0034 anti-human CD3 UCHT1 PG
TotalSeq™-B0034 anti-human CD3 UCHT1 PG
TotalSeq™-C0034 anti-human CD3 UCHT1 PG
PE anti-human CD3 UCHT1 FC
PE/Cyanine7 anti-human CD3 UCHT1 FC
KIRAVIA Blue 520™ anti-human CD3 UCHT1 FC
Spark Violet™ 538 anti-human CD3 Antibody UCHT1 FC
TotalSeq™-D0034 anti-human CD3 UCHT1 PG
Spark Blue™ 574 anti-human CD3 Antibody UCHT1 FC
GMP Pacific Blue™ anti-human CD3 UCHT1 FC
GMP PE anti-human CD3 UCHT1 FC
GMP PE/Dazzle™ 594 anti-human CD3 UCHT1 FC
Spark Violet™ 423 anti-human CD3 UCHT1 FC
GMP PE/Cyanine7 anti-human CD3 UCHT1 FC
Spark Blue™ 515 anti-human CD3 UCHT1 FC
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account