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PE anti-human CD33 Antibody

Pricing & Availability
Clone
P67.6 (See other available formats)
Regulatory Status
RUO
Other Names
Siglec-3, gp67, p67
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
P67.6_PE_CD33_Antibody_FC_080315
Human peripheral blood monocytes were stained with CD33 (clone P67.6) PE (filled histogram) or mouse IgG1, κ PE (open histogram).
  • P67.6_PE_CD33_Antibody_FC_080315
    Human peripheral blood monocytes were stained with CD33 (clone P67.6) PE (filled histogram) or mouse IgG1, κ PE (open histogram).
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366607 25 tests 117 CHF
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366608 100 tests 262 CHF
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Description

CD33, also known as Siglec-3, gp67, and p67, is a 67 kD type I transmembrane glycoprotein. It is a sialoadhesion immunoglobulin superfamily member, which is expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells, and mast cells. CD33 is absent on normal platelets, lymphocytes, erythrocytes, and hematopoietic stem cells. CD33 functions as a sialic acid-dependent cell adhesion molecule with carbohydrate/lectin binding activity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
FMY9S5 cells expressing CD33 gene.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Hoyer J, et al. 2008. Am. J. Clin. Pathol. 129:316.
Product Citations
  1. Qin K, et al. 2022. Nat Genet. 54:874. PubMed
  2. Wheeler MM, et al. 2022. Nat Commun. 13:7592. PubMed
  3. Nagpal N, et al. 2020. Cell Stem Cell. 26(6):896-909. PubMed
  4. Jacoby E, et al. 2021. NPJ Regen Med. 6:58. PubMed
  5. Guney-Esken G, et al. 2021. Stem Cell Res Ther. 12:287. PubMed
  6. Takano T, et al. 2022. Cell Rep Med. 3:100631. PubMed
  7. Vinjamur DS, et al. 2021. Nat Genet. 53:719. PubMed
  8. Merugu S, et al. 2020. Methods Enzymol. 639:289. PubMed
  9. Rao S, et al. 2021. Cell Stem Cell. 28(5):833-845.e5. PubMed
  10. Wu Y, et al. 2019. Nat Med. 25:776. PubMed
  11. Di Genua C, et al. 2020. Cancer Cell. 37(5):690-704.e8.. PubMed
  12. Lan X, et al. 2020. Molecular Cell. 81(2):239-254.e8. PubMed
  13. Ann Butler C, et al. 2021. Journal of Neurochemistry. . PubMed
  14. Zeng J, et al. 2020. Nat Med. 26:535. PubMed
  15. Roy AG, et al. 2021. Int J Mol Sci. 22:. PubMed
  16. Fujisaka Y, et al. 2018. Oncol Lett. 15:509. PubMed
  17. Sun J et al. 2018. Cell stem cell. 23(3):355-369 . PubMed
  18. Pribyl M, et al. 2020. Mol Oncol. 2.252083333. PubMed
  19. Diamantopoulos PT, et al. 2022. Cancers (Basel). 14:. PubMed
  20. Wang H, et al. 2022. Am J Cancer Res. 12:427. PubMed
RRID
AB_2566106 (BioLegend Cat. No. 366607)
AB_2566106 (BioLegend Cat. No. 366608)

Antigen Details

Structure
Ig superfamily, sialoadhesins, type I glycoprotein, 67 kD
Distribution

Myeloid progenitor, monocytes, granulocytes, dendritic cells, and mast cells.

Function
Adhesion and lectin binding activity.
Ligand/Receptor
Sugar chains containing sialic acid.
Cell Type
Dendritic cells, Granulocytes, Hematopoietic stem and progenitors, Mast cells, Monocytes
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules, Siglec Molecules
Antigen References

1. Favaloro E, et al. 1988. Br. J. Haematol. 69:163.
2. Freeman S, et al. 1995. Blood 85:2005.

Gene ID
945 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD33
Specificity Alt (DOES NOT SHOW ON TDS):
CD33
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD33 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 08.07.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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