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Purified anti-mouse CD2 Antibody

Pricing & Availability
Clone
RM2-5 (See other available formats)
Regulatory Status
RUO
Other Names
LFA-2, T11, Ly-37, SRBC-R
Isotype
Rat IgG2b, λ
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Product Citations
publications
RM2-5_Purified_021306
C57BL/6 mouse splenocytes stained with purified RM2-5
  • RM2-5_Purified_021306
    C57BL/6 mouse splenocytes stained with purified RM2-5
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100102 500 µg 233 CHF
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Description

CD2 is a 45-58 kD type I transmembrane glycoprotein, also known as LFA-2, T11 or Ly-37. It is a member of the Ig superfamily. Mouse CD2 is primarily expressed on T cells, B cells, thymocytes and NK cells. It is a ligand for CD48 and is involved in T cell activation and differentiation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
BALB/c mouse thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, Block, Costim - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: in vitro blocking of CD2-mediated cell-cell adhesion and inhibition of T cell activation1, blocking of T cell A.I.C.D.2, immunoprecipitation3, and co-induction of thymocyte maturation4. The RM2-5 antibody can block CD2-mediated cell-cell adhesion. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 100118 and 100119).

Application References

(PubMed link indicates BioLegend citation)
  1. Nakamura T, et al. 1990. J. Immunol. 145:3628. (Block)
  2. Ayroldi E, et al. 1997. Blood 89:3717. (Block)
  3. Criado G, et al. 1996. Eur. J. Immunol. 26:1228. (IP)
  4. Cibotti R, et al. 1997. Immunity 6:245. (Costim)
Product Citations
  1. Potempa M, et al. 2022. J Immunol. 208:1742. PubMed
  2. Mammadli M, et al. 2021. Cancers (Basel). 13:. PubMed
  3. Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed
RRID
AB_312649 (BioLegend Cat. No. 100102)

Antigen Details

Structure
Ig superfamily, 45-58 kD
Distribution

T cells, thymocytes, NK cells, B cells

Function
T cell activation, adhesion
Ligand/Receptor
CD48, lck
Cell Type
B cells, NK cells, T cells, Thymocytes
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis SJ, et al. 1996. Immunol. Today 17:177.
3. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.

Gene ID
12481 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD2
Specificity Alt (DOES NOT SHOW ON TDS):
CD2
App Abbreviation (DOES NOT SHOW ON TDS):
FC,IP,Block,Costim
UniProt
View information about CD2 on UniProt.org
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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