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Purified anti-mouse CD5 Antibody

Pricing & Availability
Clone
53-7.3 (See other available formats)
Regulatory Status
RUO
Other Names
Lyt-1, Ly-1, T1, Tp67, Ly-12
Isotype
Rat IgG2a, κ
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Product Citations
publications
1-53-7dot3_Purified_041106
C57BL/6 mouse splenocytes stained with purified 53-7.3, followed by anti-rat IgGs-FITC
  • 1-53-7dot3_Purified_041106
    C57BL/6 mouse splenocytes stained with purified 53-7.3, followed by anti-rat IgGs-FITC
  • 2-53-7dot3_Pure_CD5_Antibody_102218
    Fresh, frozen mouse spleen was stained with purified CD5 clone 53-7.3 conjugated and detected with a Cy3 CODEX™ oligonucleotide duplex (red). Samples were counterstained with B220 FITC (green). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
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100602 500 µg 190 CHF
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Description

CD5 is a 67 kD protein, also known as Lyt-1, Ly-1, T1, Tp67, or Ly-12. It is a member of the scavenger receptor cysteine-rich protein superfamily (SRCR) and primarily expressed on thymocytes, T cells, and B-1 cells. Although mature α/β T cells express high levels of CD5, very few γ/δ T cells express this antigen. The interaction of CD5 with CD72, gp35-37, TCR, or BCR is involved in T and B cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, CyTOF® - Verified
IHC-P, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, and immunohistochemistry2 of acetone-fixed frozen tissue sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  2. Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (FC, IHC)
  3. Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370.
Product Citations
  1. Michela Miani et al. 2018. Cell metabolism. 28(4):557-572 . PubMed
  2. Kang YA, et al. 2023. J Exp Med. 220:. PubMed
  3. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  4. Seifert HA, et al. 2018. Metab Brain Dis. 33:1599. PubMed
  5. Prado C, et al. 2021. J Neuroinflammation. 18:292. PubMed
  6. Niss Arfelt K, et al. 2017. Blood. 129:866. PubMed
  7. Peng V, et al. 2020. J Biol Chem. 295:14866. PubMed
  8. Doorduijn EM, et al. 2018. Front Immunol. 0.416666667. PubMed
  9. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  10. Formaglio P, et al. 2021. Immunity. 54:2724. PubMed
  11. Liu H, et al. 2020. J Immunol. 205:1207. PubMed
  12. Sprouse ML, et al. 2018. JCI Insight. 3:e97322. PubMed
  13. Lee JY, et al. 2018. Front Immunol. 0.678472222. PubMed
  14. Yates K, et al. 2018. Proc Natl Acad Sci U S A. 115:2162. PubMed
  15. Wang HC, et al. 2017. J Immunol. 198:3149. PubMed
  16. Parigi SM, et al. 2018. Sci Rep. 0.440277778. PubMed
  17. Kawabe T, et al. 2017. Sci Immunol. 2:eaam9315. PubMed
  18. Goltsev Y et al. 2018. Cell. 174(4):968-981 . PubMed
  19. Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed
  20. Cole C, et al. 2018. Nucleic Acids Res. 46:e62. PubMed
  21. Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed
  22. Kästele V, et al. 2021. Mucosal Immunol. 14:717. PubMed
RRID
AB_312731 (BioLegend Cat. No. 100602)

Antigen Details

Structure
Member of the scavenger receptor cysteine-rich protein superfamily (SRCR), 67 kD
Distribution

Thymocytes, T cells, B-1 cells

Function
Negative regulator of T-B cell interaction
Ligand/Receptor
CD72, gp35-37
Cell Type
B cells, T cells, Thymocytes
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kipps TJ. 1988. Adv. Immunol. 47:117.
3. Antin JH, et al. 1985. J. Immunol. 136:505.
4. Tarakhovsky A, et al. 1995. Science 269:535.

Gene ID
12507 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD5
Specificity Alt (DOES NOT SHOW ON TDS):
CD5
App Abbreviation (DOES NOT SHOW ON TDS):
FC,IHC-F,CyTOF®,IHC-P,IP
UniProt
View information about CD5 on UniProt.org
Go To Top Version: 2    Revision Date: 10.24.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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