Ultra-LEAF™ Purified anti-mouse CD254 (TRANCE, RANKL) Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

NSO-derived recombinant mouse TRANCE

Formulation

0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.

Endotoxin Level

Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
IP, WB, Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The IK22/5 antibody has been reported to block the binding of RANK ligand-Fc to RANK. Additional reported applications (for the relevant formats) include: immunoprecipitation², Western blotting², and blocking of ligand binding^1,2,4. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 510009-510014).

Application References

(PubMed link indicates BioLegend citation)

1. Miyahira Y, et al. 2003. J. Immunol. 171:6344. (Block)
2. Personal communication (Block IP WB)
3. Gao Y, et al. 2007. J. Clin. Invest. 117:122.
4. Kamijo S, et al. 2006. Biochem. Biophys. Res. Commun. 347:124. (Block)
5. Fionda C, et al. 2007. J. Immunol. 178:4039.
6. Haslam SZ, et al. 2008. Endocrinology. 149:2098. (Block) PubMed

RRID

AB_2832814 (BioLegend Cat. No. 510014)
AB_2832814 (BioLegend Cat. No. 510012)
AB_2832814 (BioLegend Cat. No. 510013)
AB_2832814 (BioLegend Cat. No. 510009)
AB_2832814 (BioLegend Cat. No. 510010)
AB_2832814 (BioLegend Cat. No. 510011)

Antigen Details

Structure

TNF superfamily; 19 kD (Mammalian)

Distribution

Activated T cells, osteoclasts

Function

Regulates T and dendritic cell interactions, and osteoclast differentiation

Ligand/Receptor

RANK, OPG

Bioactivity

Activates JNK and NF-κB; involved in bone resorption; induces the expression of IL-1, IL-6, IL-12, IL-15 in dendritic cells

Cell Sources

Activated T cells, myeloid bone marrow precursors, stromal fibroblasts, synovial cells, osteoblasts

Cell Targets

Dendritic cells, osteoclasts

Receptors

ODFR, ODAR, TRANCE-R

Cell Type

Osteoclasts, T cells

Biology Area

Cell Biology, Immunology

Molecular Family

CD Molecules, Cytokines/Chemokines

Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Josien R, et al. 1999. J. Immunol. 162:2562.
3. Takayanagi H, et al. 2000. Nature 408:600.
4. Wong B, et al. 1999. Mol. Cell. 4:1041.

Regulation

Type II integral membrane protein processed by TACE for secretion; upregulated upon TCR engagement

Gene ID

21943 View all products for this Gene ID

Specificity (DOES NOT SHOW ON TDS):

CD254

Specificity Alt (DOES NOT SHOW ON TDS):

CD254

App Abbreviation (DOES NOT SHOW ON TDS):

FC,IP,WB,Block

UniProt

View information about CD254 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

Certificate of Analysis