Alexa Fluor 488 anti-human CD18 Antibody anti-CD18

Pricing & Availability

Clone: 1B4/CD18 (See other available formats)
Regulatory Status: RUO
Other Names: Integrin beta-2, integrin beta chain, beta 2
Isotype: Mouse IgG2a, κ
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Product Citations: publications

1B4slashCD18_A488_CD18_Antibody_082517 — Human peripheral blood lymphocytes were stained with CD18 (clone 1B4/CD18) Alexa Fluor® 488 (filled histogram) or mouse IgG2a, κ Alexa Fluor® 488 isotype control (open histogram).

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373403

25 tests

147 CHF

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373404

100 tests

323 CHF

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Description

CD18 is a 90-95 kD type I transmembrane protein also known as integrin β2 subunit, LFA-1 β subunit, and β2 integrin. CD18 non-covalently associates with CD11a, CD11b or CD11c. CD18 is expressed on all leukocytes. CD18 and associated α chains function in adhesion and signaling in hematopoietic cells.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human peripheral blood lymphocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Blue Laser (488 nm)
Application Notes: The 1B4/CD18 antibody was able to partially block the Ts1/18 antibody, indicating the two anti-CD18 antibodies recognize distinct but overlapping epitopes.
RRID: AB_2716019 (BioLegend Cat. No. 373403)
AB_2716019 (BioLegend Cat. No. 373404)

Antigen Details

Structure: Integrin, type I transmembrane glycoprotein
Distribution: Leukocytes
Function: Adhesion and signaling in hematopoietic cells
Interaction: Associates with CD11a, CD11b, or CD11c
Ligand/Receptor: ICAMs
Cell Type: Leukocytes, Tregs
Biology Area: Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Hogg N, et al. 1999. J. Clin. Invest. 103:97.
2. Anderson DC, et al. 1987. Annu. Rev. Med. 38:175.
3. Springer TA. 1994. Cell 76:301.
Gene ID: 3689 View all products for this Gene ID
UniProt: View information about CD18 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD18 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD18	1B4/CD18	FC
APC anti-human CD18	1B4/CD18	FC
PE/Cyanine7 anti-human CD18	1B4/CD18	FC
Alexa Fluor® 488 anti-human CD18	1B4/CD18	FC
PE anti-human CD18	1B4/CD18	FC

Certificate of Analysis

Go To Top Version: 0 Revision Date: 08.29.2017

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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